The β1 adrenergic effects of antibodies against the C-terminal end of the ribosomal P2β protein of Trypanosoma cruzi associate with a specific pattern of epitope recognition
BALB/c mice immunized with recombinant Trypanosoma cruzi ribosomal P2β protein (TcP2β) develop a strong and specific antibody response against its 13 residue-long C-terminal epitope (peptide R13: EEEDDDMGFGLFD) that has a concomitant β1-adrenergic stimulating activity. However, other animals that un...
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2005
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00099104_v142_n1_p140_Bergami http://hdl.handle.net/20.500.12110/paper_00099104_v142_n1_p140_Bergami |
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paper:paper_00099104_v142_n1_p140_Bergami2023-06-08T14:34:07Z The β1 adrenergic effects of antibodies against the C-terminal end of the ribosomal P2β protein of Trypanosoma cruzi associate with a specific pattern of epitope recognition β1-adrenergic receptor Pathogenic antibodies Ribosomal P proteins Trypanosoma cruzi beta 1 adrenergic receptor epitope glutamylglutamylglutamylaspartylaspartylaspartylmethionylglycylphenylalanylglycylleucylphenylalanylaspartic acid peptide protein antibody protein TcP2beta protozoal protein ribosome protein unclassified drug autoantibody beta 1 adrenergic receptor epitope immunoglobulin G protozoon antibody recombinant protein ribosome protein adrenergic activity amino acid sequence animal experiment animal model antigen recognition article carboxy terminal sequence controlled study electrocardiography epitope mapping immune response immunization immunological tolerance mouse nonhuman priority journal protein analysis protein motif supraventricular tachycardia Trypanosoma cruzi animal autoimmunity Bagg albino mouse Chagas disease immunology methodology pathophysiology Trypanosoma cruzi Animals Antibodies, Protozoan Autoantibodies Autoimmunity Chagas Disease Electrocardiography Epitope Mapping Epitopes Immunoglobulin G Mice Mice, Inbred BALB C Protozoan Proteins Receptors, Adrenergic, beta-1 Recombinant Proteins Ribosomal Proteins Trypanosoma cruzi BALB/c mice immunized with recombinant Trypanosoma cruzi ribosomal P2β protein (TcP2β) develop a strong and specific antibody response against its 13 residue-long C-terminal epitope (peptide R13: EEEDDDMGFGLFD) that has a concomitant β1-adrenergic stimulating activity. However, other animals that undergo similar immunizations seem tolerant to this epitope. To evaluate further the antibody response against the ribosomal P proteins, 25 BALB/c and 25 Swiss mice were immunized with TcP2β. From the 50 animals, 31 developed a positive anti-R13 response, whereas 19 were non-responsive. From the 31 anti-R13 positive mice, 25 had anti-R13 antibodies that recognized the discontinuous motif ExDDxGF, and their presence correlated with the recording of supraventricular tachycardia. The other six had anti-R13 antibodies but with a normal electrocardiographic recording. These anti-R13 antibodies recognized the motif DDxGF shared by mammals and T. cruzi and proved to be a true anti-P autoantibody because they were similar to those elicited in Swiss, but not in BALB/c mice, by immunization with the C-terminal portion of the mouse ribosomal P protein. Our results show that the recognition of the glutamic acid in position 3 of peptide R13 defines the ability of anti-R13 antibodies to react with the motif AESDE of the second extracellular loop of the β1-adrenergic receptor, setting the molecular basis for their pathogenic β1 adrenoceptor stimulating activity. © 2005 British Society for Immunology. 2005 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00099104_v142_n1_p140_Bergami http://hdl.handle.net/20.500.12110/paper_00099104_v142_n1_p140_Bergami |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
β1-adrenergic receptor Pathogenic antibodies Ribosomal P proteins Trypanosoma cruzi beta 1 adrenergic receptor epitope glutamylglutamylglutamylaspartylaspartylaspartylmethionylglycylphenylalanylglycylleucylphenylalanylaspartic acid peptide protein antibody protein TcP2beta protozoal protein ribosome protein unclassified drug autoantibody beta 1 adrenergic receptor epitope immunoglobulin G protozoon antibody recombinant protein ribosome protein adrenergic activity amino acid sequence animal experiment animal model antigen recognition article carboxy terminal sequence controlled study electrocardiography epitope mapping immune response immunization immunological tolerance mouse nonhuman priority journal protein analysis protein motif supraventricular tachycardia Trypanosoma cruzi animal autoimmunity Bagg albino mouse Chagas disease immunology methodology pathophysiology Trypanosoma cruzi Animals Antibodies, Protozoan Autoantibodies Autoimmunity Chagas Disease Electrocardiography Epitope Mapping Epitopes Immunoglobulin G Mice Mice, Inbred BALB C Protozoan Proteins Receptors, Adrenergic, beta-1 Recombinant Proteins Ribosomal Proteins Trypanosoma cruzi |
| spellingShingle |
β1-adrenergic receptor Pathogenic antibodies Ribosomal P proteins Trypanosoma cruzi beta 1 adrenergic receptor epitope glutamylglutamylglutamylaspartylaspartylaspartylmethionylglycylphenylalanylglycylleucylphenylalanylaspartic acid peptide protein antibody protein TcP2beta protozoal protein ribosome protein unclassified drug autoantibody beta 1 adrenergic receptor epitope immunoglobulin G protozoon antibody recombinant protein ribosome protein adrenergic activity amino acid sequence animal experiment animal model antigen recognition article carboxy terminal sequence controlled study electrocardiography epitope mapping immune response immunization immunological tolerance mouse nonhuman priority journal protein analysis protein motif supraventricular tachycardia Trypanosoma cruzi animal autoimmunity Bagg albino mouse Chagas disease immunology methodology pathophysiology Trypanosoma cruzi Animals Antibodies, Protozoan Autoantibodies Autoimmunity Chagas Disease Electrocardiography Epitope Mapping Epitopes Immunoglobulin G Mice Mice, Inbred BALB C Protozoan Proteins Receptors, Adrenergic, beta-1 Recombinant Proteins Ribosomal Proteins Trypanosoma cruzi The β1 adrenergic effects of antibodies against the C-terminal end of the ribosomal P2β protein of Trypanosoma cruzi associate with a specific pattern of epitope recognition |
| topic_facet |
β1-adrenergic receptor Pathogenic antibodies Ribosomal P proteins Trypanosoma cruzi beta 1 adrenergic receptor epitope glutamylglutamylglutamylaspartylaspartylaspartylmethionylglycylphenylalanylglycylleucylphenylalanylaspartic acid peptide protein antibody protein TcP2beta protozoal protein ribosome protein unclassified drug autoantibody beta 1 adrenergic receptor epitope immunoglobulin G protozoon antibody recombinant protein ribosome protein adrenergic activity amino acid sequence animal experiment animal model antigen recognition article carboxy terminal sequence controlled study electrocardiography epitope mapping immune response immunization immunological tolerance mouse nonhuman priority journal protein analysis protein motif supraventricular tachycardia Trypanosoma cruzi animal autoimmunity Bagg albino mouse Chagas disease immunology methodology pathophysiology Trypanosoma cruzi Animals Antibodies, Protozoan Autoantibodies Autoimmunity Chagas Disease Electrocardiography Epitope Mapping Epitopes Immunoglobulin G Mice Mice, Inbred BALB C Protozoan Proteins Receptors, Adrenergic, beta-1 Recombinant Proteins Ribosomal Proteins Trypanosoma cruzi |
| description |
BALB/c mice immunized with recombinant Trypanosoma cruzi ribosomal P2β protein (TcP2β) develop a strong and specific antibody response against its 13 residue-long C-terminal epitope (peptide R13: EEEDDDMGFGLFD) that has a concomitant β1-adrenergic stimulating activity. However, other animals that undergo similar immunizations seem tolerant to this epitope. To evaluate further the antibody response against the ribosomal P proteins, 25 BALB/c and 25 Swiss mice were immunized with TcP2β. From the 50 animals, 31 developed a positive anti-R13 response, whereas 19 were non-responsive. From the 31 anti-R13 positive mice, 25 had anti-R13 antibodies that recognized the discontinuous motif ExDDxGF, and their presence correlated with the recording of supraventricular tachycardia. The other six had anti-R13 antibodies but with a normal electrocardiographic recording. These anti-R13 antibodies recognized the motif DDxGF shared by mammals and T. cruzi and proved to be a true anti-P autoantibody because they were similar to those elicited in Swiss, but not in BALB/c mice, by immunization with the C-terminal portion of the mouse ribosomal P protein. Our results show that the recognition of the glutamic acid in position 3 of peptide R13 defines the ability of anti-R13 antibodies to react with the motif AESDE of the second extracellular loop of the β1-adrenergic receptor, setting the molecular basis for their pathogenic β1 adrenoceptor stimulating activity. © 2005 British Society for Immunology. |
| title |
The β1 adrenergic effects of antibodies against the C-terminal end of the ribosomal P2β protein of Trypanosoma cruzi associate with a specific pattern of epitope recognition |
| title_short |
The β1 adrenergic effects of antibodies against the C-terminal end of the ribosomal P2β protein of Trypanosoma cruzi associate with a specific pattern of epitope recognition |
| title_full |
The β1 adrenergic effects of antibodies against the C-terminal end of the ribosomal P2β protein of Trypanosoma cruzi associate with a specific pattern of epitope recognition |
| title_fullStr |
The β1 adrenergic effects of antibodies against the C-terminal end of the ribosomal P2β protein of Trypanosoma cruzi associate with a specific pattern of epitope recognition |
| title_full_unstemmed |
The β1 adrenergic effects of antibodies against the C-terminal end of the ribosomal P2β protein of Trypanosoma cruzi associate with a specific pattern of epitope recognition |
| title_sort |
β1 adrenergic effects of antibodies against the c-terminal end of the ribosomal p2β protein of trypanosoma cruzi associate with a specific pattern of epitope recognition |
| publishDate |
2005 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00099104_v142_n1_p140_Bergami http://hdl.handle.net/20.500.12110/paper_00099104_v142_n1_p140_Bergami |
| _version_ |
1768545860400971776 |