Studies on porphyrin biosynthesis in lead-intoxicated rabbits

The present report deals with studies on porphyrins and porphyrinogen carboxylyase of red cells and urinary porphyrins from lead-intoxicated rabbits. It was shown that the free erythrocyte porphyrins are a mixture of protoporphyrin 9, the main component, and minor proportions of Coproporphyrin, Urop...

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Autor principal: Grinstein, Moisés
Publicado: 1976
Materias:
porphyrin
uroporphyrinogen decarboxylase
in vitro study
lead poisoning
theoretical study
Animal
Erythrocytes
Female
Lead Poisoning
Male
Porphyrinogens
Porphyrins
Rabbits
Uroporphyrinogen Decarboxylase
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00098981_v69_n3_p375_DeViale
http://hdl.handle.net/20.500.12110/paper_00098981_v69_n3_p375_DeViale
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_00098981_v69_n3_p375_DeViale
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spelling paper:paper_00098981_v69_n3_p375_DeViale2023-06-08T14:34:06Z Studies on porphyrin biosynthesis in lead-intoxicated rabbits Grinstein, Moisés porphyrin uroporphyrinogen decarboxylase in vitro study lead poisoning theoretical study Animal Erythrocytes Female Lead Poisoning Male Porphyrinogens Porphyrins Rabbits Uroporphyrinogen Decarboxylase The present report deals with studies on porphyrins and porphyrinogen carboxylyase of red cells and urinary porphyrins from lead-intoxicated rabbits. It was shown that the free erythrocyte porphyrins are a mixture of protoporphyrin 9, the main component, and minor proportions of Coproporphyrin, Uroporphyrin III and Phyriaporphyrin. Analysis of the urinary porphyrins demonstrates the presence of Coproporphyrin III as the major component, together with 15-20% of other porphyrins: 10-14% 5-COOH, 1-2% 6-COOH, 2-3% 7-COOH porphyrin and 1-2% Uroporphyrin III. We have not been able to detect an increase of Uroporphyrin I. Assays of porphyrinogen carboxy-lyase activity in hemolysate supernatant using Uroporphyrinogen III and Phyriaporphyrinogen (Phyria'gen) III as substrates, showed the existence of a slight decrease of both decarboxylase activities, being more affected during the second stage, the Phyria'gen decarboxylation. A possible regulation mechanism responsible for the porphyrin picture is discussed. © 1976. Fil:Grinstein, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1976 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00098981_v69_n3_p375_DeViale http://hdl.handle.net/20.500.12110/paper_00098981_v69_n3_p375_DeViale
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic porphyrin
uroporphyrinogen decarboxylase
in vitro study
lead poisoning
theoretical study
Animal
Erythrocytes
Female
Lead Poisoning
Male
Porphyrinogens
Porphyrins
Rabbits
Uroporphyrinogen Decarboxylase
spellingShingle porphyrin
uroporphyrinogen decarboxylase
in vitro study
lead poisoning
theoretical study
Animal
Erythrocytes
Female
Lead Poisoning
Male
Porphyrinogens
Porphyrins
Rabbits
Uroporphyrinogen Decarboxylase
Grinstein, Moisés
Studies on porphyrin biosynthesis in lead-intoxicated rabbits
topic_facet porphyrin
uroporphyrinogen decarboxylase
in vitro study
lead poisoning
theoretical study
Animal
Erythrocytes
Female
Lead Poisoning
Male
Porphyrinogens
Porphyrins
Rabbits
Uroporphyrinogen Decarboxylase
description The present report deals with studies on porphyrins and porphyrinogen carboxylyase of red cells and urinary porphyrins from lead-intoxicated rabbits. It was shown that the free erythrocyte porphyrins are a mixture of protoporphyrin 9, the main component, and minor proportions of Coproporphyrin, Uroporphyrin III and Phyriaporphyrin. Analysis of the urinary porphyrins demonstrates the presence of Coproporphyrin III as the major component, together with 15-20% of other porphyrins: 10-14% 5-COOH, 1-2% 6-COOH, 2-3% 7-COOH porphyrin and 1-2% Uroporphyrin III. We have not been able to detect an increase of Uroporphyrin I. Assays of porphyrinogen carboxy-lyase activity in hemolysate supernatant using Uroporphyrinogen III and Phyriaporphyrinogen (Phyria'gen) III as substrates, showed the existence of a slight decrease of both decarboxylase activities, being more affected during the second stage, the Phyria'gen decarboxylation. A possible regulation mechanism responsible for the porphyrin picture is discussed. © 1976.
author Grinstein, Moisés
author_facet Grinstein, Moisés
author_sort Grinstein, Moisés
title Studies on porphyrin biosynthesis in lead-intoxicated rabbits
title_short Studies on porphyrin biosynthesis in lead-intoxicated rabbits
title_full Studies on porphyrin biosynthesis in lead-intoxicated rabbits
title_fullStr Studies on porphyrin biosynthesis in lead-intoxicated rabbits
title_full_unstemmed Studies on porphyrin biosynthesis in lead-intoxicated rabbits
title_sort studies on porphyrin biosynthesis in lead-intoxicated rabbits
publishDate 1976
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00098981_v69_n3_p375_DeViale
http://hdl.handle.net/20.500.12110/paper_00098981_v69_n3_p375_DeViale
work_keys_str_mv AT grinsteinmoises studiesonporphyrinbiosynthesisinleadintoxicatedrabbits
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