R-Spondin3 is associated with basal-progenitor behavior in normal and tumor mammary cells

R-spondin3 (RSPO3) is a member of a family of secreted proteins that enhance Wnt signaling pathways in diverse processes, including cancer. However, the role of RSPO3 in mammary gland and breast cancer development remains unclear. In this study, we show that RSPO3 is expressed in the basal stem cell...

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Publicado:	2018
Materias:	G protein coupled receptor leucine rich repeat containing G protein coupled receptor protein R spondin3 transcription factor RUNX unclassified drug adult animal cell animal experiment animal model animal tissue Article basal like breast cancer breast carcinogenesis breast cell cancer cell cancer growth cell differentiation cell migration controlled study epithelial mesenchymal transition female gene gene knockdown gene overexpression gene targeting human human cell human tissue in vivo study mouse nonhuman priority journal protein expression RSPO3 gene stem cell Wnt signaling
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00085472_v78_n16_p4497_Tocci http://hdl.handle.net/20.500.12110/paper_00085472_v78_n16_p4497_Tocci
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00085472_v78_n16_p4497_Tocci
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spelling	paper:paper_00085472_v78_n16_p4497_Tocci2023-06-08T14:32:09Z R-Spondin3 is associated with basal-progenitor behavior in normal and tumor mammary cells G protein coupled receptor leucine rich repeat containing G protein coupled receptor protein R spondin3 transcription factor RUNX unclassified drug adult animal cell animal experiment animal model animal tissue Article basal like breast cancer breast carcinogenesis breast cell cancer cell cancer growth cell differentiation cell migration controlled study epithelial mesenchymal transition female gene gene knockdown gene overexpression gene targeting human human cell human tissue in vivo study mouse nonhuman priority journal protein expression RSPO3 gene stem cell Wnt signaling R-spondin3 (RSPO3) is a member of a family of secreted proteins that enhance Wnt signaling pathways in diverse processes, including cancer. However, the role of RSPO3 in mammary gland and breast cancer development remains unclear. In this study, we show that RSPO3 is expressed in the basal stem cell–enriched compartment of normal mouse mammary glands but is absent from committed mature luminal cells in which exogenous RSPO3 impairs lactogenic differentiation. RSPO3 knockdown in basal-like mouse mammary tumor cells reduced canonical Wnt signaling, epithelial-to-mesenchymal transition-like features, migration capacity, and tumor formation in vivo. Conversely, RSPO3 overexpression, which was associated with some LGR and RUNX factors, highly correlated with the basal-like subtype among patients with breast cancer. Thus, we identified RSPO3 as a novel key modulator of breast cancer development and a potential target for treatment of basal-like breast cancers. Significance: These findings identify RSPO3 as a potential therapetuic target in basal-like breast cancers. Graphical Abstract: http://cancerres.aacrjournals.org/content/canres/78/16/4497/F1.large.jpg. 2018 American Association for Cancer Research. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00085472_v78_n16_p4497_Tocci http://hdl.handle.net/20.500.12110/paper_00085472_v78_n16_p4497_Tocci
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	G protein coupled receptor leucine rich repeat containing G protein coupled receptor protein R spondin3 transcription factor RUNX unclassified drug adult animal cell animal experiment animal model animal tissue Article basal like breast cancer breast carcinogenesis breast cell cancer cell cancer growth cell differentiation cell migration controlled study epithelial mesenchymal transition female gene gene knockdown gene overexpression gene targeting human human cell human tissue in vivo study mouse nonhuman priority journal protein expression RSPO3 gene stem cell Wnt signaling
spellingShingle	G protein coupled receptor leucine rich repeat containing G protein coupled receptor protein R spondin3 transcription factor RUNX unclassified drug adult animal cell animal experiment animal model animal tissue Article basal like breast cancer breast carcinogenesis breast cell cancer cell cancer growth cell differentiation cell migration controlled study epithelial mesenchymal transition female gene gene knockdown gene overexpression gene targeting human human cell human tissue in vivo study mouse nonhuman priority journal protein expression RSPO3 gene stem cell Wnt signaling R-Spondin3 is associated with basal-progenitor behavior in normal and tumor mammary cells
topic_facet	G protein coupled receptor leucine rich repeat containing G protein coupled receptor protein R spondin3 transcription factor RUNX unclassified drug adult animal cell animal experiment animal model animal tissue Article basal like breast cancer breast carcinogenesis breast cell cancer cell cancer growth cell differentiation cell migration controlled study epithelial mesenchymal transition female gene gene knockdown gene overexpression gene targeting human human cell human tissue in vivo study mouse nonhuman priority journal protein expression RSPO3 gene stem cell Wnt signaling
description	R-spondin3 (RSPO3) is a member of a family of secreted proteins that enhance Wnt signaling pathways in diverse processes, including cancer. However, the role of RSPO3 in mammary gland and breast cancer development remains unclear. In this study, we show that RSPO3 is expressed in the basal stem cell–enriched compartment of normal mouse mammary glands but is absent from committed mature luminal cells in which exogenous RSPO3 impairs lactogenic differentiation. RSPO3 knockdown in basal-like mouse mammary tumor cells reduced canonical Wnt signaling, epithelial-to-mesenchymal transition-like features, migration capacity, and tumor formation in vivo. Conversely, RSPO3 overexpression, which was associated with some LGR and RUNX factors, highly correlated with the basal-like subtype among patients with breast cancer. Thus, we identified RSPO3 as a novel key modulator of breast cancer development and a potential target for treatment of basal-like breast cancers. Significance: These findings identify RSPO3 as a potential therapetuic target in basal-like breast cancers. Graphical Abstract: http://cancerres.aacrjournals.org/content/canres/78/16/4497/F1.large.jpg. 2018 American Association for Cancer Research.
title	R-Spondin3 is associated with basal-progenitor behavior in normal and tumor mammary cells
title_short	R-Spondin3 is associated with basal-progenitor behavior in normal and tumor mammary cells
title_full	R-Spondin3 is associated with basal-progenitor behavior in normal and tumor mammary cells
title_fullStr	R-Spondin3 is associated with basal-progenitor behavior in normal and tumor mammary cells
title_full_unstemmed	R-Spondin3 is associated with basal-progenitor behavior in normal and tumor mammary cells
title_sort	r-spondin3 is associated with basal-progenitor behavior in normal and tumor mammary cells
publishDate	2018
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00085472_v78_n16_p4497_Tocci http://hdl.handle.net/20.500.12110/paper_00085472_v78_n16_p4497_Tocci
_version_	1768541919711854592

R-Spondin3 is associated with basal-progenitor behavior in normal and tumor mammary cells

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