Effects of adrenalectomy and acute replacement by corticosteroids on distal acidification

The role of adrenocortical steroids in distal nephron acidification was studied in rats by measuring urine minus blood PCO2 differences (U-B PCO2) in control, sham-operated, and adrenalectomized (ADX) animals. Operations were performed 48 h before experiments. During the experiments, all rats receiv...

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Detalles Bibliográficos
Autor principal:	Ansaldo, Martín
Publicado:	1989
Materias:	18 hydroxycorticosterone aldosterone bicarbonate corticosterone adrenalectomy animal cell animal experiment blood gas controlled study kidney function male nonhuman priority journal rat urine acidification
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00084212_v67_n6_p607_Damasco http://hdl.handle.net/20.500.12110/paper_00084212_v67_n6_p607_Damasco
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00084212_v67_n6_p607_Damasco
record_format	dspace
spelling	paper:paper_00084212_v67_n6_p607_Damasco2023-06-08T14:32:01Z Effects of adrenalectomy and acute replacement by corticosteroids on distal acidification Ansaldo, Martín 18 hydroxycorticosterone aldosterone bicarbonate corticosterone adrenalectomy animal cell animal experiment blood gas controlled study kidney function male nonhuman priority journal rat urine acidification The role of adrenocortical steroids in distal nephron acidification was studied in rats by measuring urine minus blood PCO2 differences (U-B PCO2) in control, sham-operated, and adrenalectomized (ADX) animals. Operations were performed 48 h before experiments. During the experiments, all rats received an infusion of 0.35-0.60 M NaHCO3, leading to urine bicarbonate concentrations in the order of 100-200 mM. Adrenalectomized rats had significantly decreased U-B PCO2 (11.9 ± 1.99 mmHg; 1 mmHg = 133.3 Pa) with respect to sham-operated rats (39.9 ± 1.26 mmHg). In another series, ADX rats received supplements of the adrenal steroids corticosterone, aldosterone, and 18-hydroxycorticosterone 100 min before the experiment. U-B PCO2 increased after hormone administration: corticosterone, 30.0 ± 2.13 mmHg; aldosterone, 26.6 ± 1.74 mmHg; 18-hydroxycorticosterone, 29.0 ± 1.60 mmHg; but none restored these values to normal. Combinations of two hormones were also used; only aldosterone + corticosterone restored U-B PCO2 to normal: 39.0 ± 1.66 mmHg. Renal phosphate excretion (but not urine phosphate levels) decreased significantly in ADX as compared with sham-operated rats. Extracellular volume was not significantly affected in ADX rats, which received ad libitum 0.9% NaCl for drinking. It is concluded that distal tubular acidification, as evaluated by U-B PCO2, is dependent on cortical steroids. Fil:Ansaldo, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1989 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00084212_v67_n6_p607_Damasco http://hdl.handle.net/20.500.12110/paper_00084212_v67_n6_p607_Damasco
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	18 hydroxycorticosterone aldosterone bicarbonate corticosterone adrenalectomy animal cell animal experiment blood gas controlled study kidney function male nonhuman priority journal rat urine acidification
spellingShingle	18 hydroxycorticosterone aldosterone bicarbonate corticosterone adrenalectomy animal cell animal experiment blood gas controlled study kidney function male nonhuman priority journal rat urine acidification Ansaldo, Martín Effects of adrenalectomy and acute replacement by corticosteroids on distal acidification
topic_facet	18 hydroxycorticosterone aldosterone bicarbonate corticosterone adrenalectomy animal cell animal experiment blood gas controlled study kidney function male nonhuman priority journal rat urine acidification
description	The role of adrenocortical steroids in distal nephron acidification was studied in rats by measuring urine minus blood PCO2 differences (U-B PCO2) in control, sham-operated, and adrenalectomized (ADX) animals. Operations were performed 48 h before experiments. During the experiments, all rats received an infusion of 0.35-0.60 M NaHCO3, leading to urine bicarbonate concentrations in the order of 100-200 mM. Adrenalectomized rats had significantly decreased U-B PCO2 (11.9 ± 1.99 mmHg; 1 mmHg = 133.3 Pa) with respect to sham-operated rats (39.9 ± 1.26 mmHg). In another series, ADX rats received supplements of the adrenal steroids corticosterone, aldosterone, and 18-hydroxycorticosterone 100 min before the experiment. U-B PCO2 increased after hormone administration: corticosterone, 30.0 ± 2.13 mmHg; aldosterone, 26.6 ± 1.74 mmHg; 18-hydroxycorticosterone, 29.0 ± 1.60 mmHg; but none restored these values to normal. Combinations of two hormones were also used; only aldosterone + corticosterone restored U-B PCO2 to normal: 39.0 ± 1.66 mmHg. Renal phosphate excretion (but not urine phosphate levels) decreased significantly in ADX as compared with sham-operated rats. Extracellular volume was not significantly affected in ADX rats, which received ad libitum 0.9% NaCl for drinking. It is concluded that distal tubular acidification, as evaluated by U-B PCO2, is dependent on cortical steroids.
author	Ansaldo, Martín
author_facet	Ansaldo, Martín
author_sort	Ansaldo, Martín
title	Effects of adrenalectomy and acute replacement by corticosteroids on distal acidification
title_short	Effects of adrenalectomy and acute replacement by corticosteroids on distal acidification
title_full	Effects of adrenalectomy and acute replacement by corticosteroids on distal acidification
title_fullStr	Effects of adrenalectomy and acute replacement by corticosteroids on distal acidification
title_full_unstemmed	Effects of adrenalectomy and acute replacement by corticosteroids on distal acidification
title_sort	effects of adrenalectomy and acute replacement by corticosteroids on distal acidification
publishDate	1989
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00084212_v67_n6_p607_Damasco http://hdl.handle.net/20.500.12110/paper_00084212_v67_n6_p607_Damasco
work_keys_str_mv	AT ansaldomartin effectsofadrenalectomyandacutereplacementbycorticosteroidsondistalacidification
_version_	1768544021382168576

Effects of adrenalectomy and acute replacement by corticosteroids on distal acidification

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