Foetal infections with bovine viral diarrhoea virus in argentina

The frequency of isolation of bovine viral diarrhoea virus (BVDV) from primary tissue cultures and organs from bovine foetuses was studied between 1992 and 1994. Around 25% of primary tissue cultures were BVDV positive. Primär)' testis cultures were inoculated with homogenates of spleen, kidney...

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Publicado: 1996
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Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00071935_v152_n2_p175_Munoz
http://hdl.handle.net/20.500.12110/paper_00071935_v152_n2_p175_Munoz
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spelling paper:paper_00071935_v152_n2_p175_Munoz2023-06-08T14:31:39Z Foetal infections with bovine viral diarrhoea virus in argentina Argentina BVDV Foetal infections Pestivirus Prevalence animal animal disease Argentina article Bovine diarrhea virus cattle cattle disease female fetus disease isolation and purification male prevalence virology Animals Argentina Bovine Virus Diarrhea-Mucosal Disease Cattle Diarrhea Viruses, Bovine Viral Female Fetal Diseases Male Prevalence The frequency of isolation of bovine viral diarrhoea virus (BVDV) from primary tissue cultures and organs from bovine foetuses was studied between 1992 and 1994. Around 25% of primary tissue cultures were BVDV positive. Primär)' testis cultures were inoculated with homogenates of spleen, kidney, lung and liver from 52 foetuses. Cells were passaged twice and BVDV antigen investigated by indirect immunofluorescence. Non-cytopathic BVDV was detected in at least one organ in 11/52 foetuses (21.2%): 6/10 spleens, 4/7 kidneys, 7/9 lungs and 3/5 livers. Cytopathic BVDV was detected in lung and kidney from two foetuses. Since only gamma-irradiated sera are used in the laboratory and only inactivated BVDV vaccines are applied in Argentina, it was concluded that these isolations represented field infections. In addition to the 11 virus positive foetuses, two foetuses were positive for BVDV antibodies, which suggested a 25% prevalence of infection. These results stress the need for disease control on a herd basis and the requirement for biological reagents of bovine origin for the detection of BVDV. © 199GBailIièreTindall. 1996 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00071935_v152_n2_p175_Munoz http://hdl.handle.net/20.500.12110/paper_00071935_v152_n2_p175_Munoz
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Argentina
BVDV
Foetal infections
Pestivirus
Prevalence
animal
animal disease
Argentina
article
Bovine diarrhea virus
cattle
cattle disease
female
fetus disease
isolation and purification
male
prevalence
virology
Animals
Argentina
Bovine Virus Diarrhea-Mucosal Disease
Cattle
Diarrhea Viruses, Bovine Viral
Female
Fetal Diseases
Male
Prevalence
spellingShingle Argentina
BVDV
Foetal infections
Pestivirus
Prevalence
animal
animal disease
Argentina
article
Bovine diarrhea virus
cattle
cattle disease
female
fetus disease
isolation and purification
male
prevalence
virology
Animals
Argentina
Bovine Virus Diarrhea-Mucosal Disease
Cattle
Diarrhea Viruses, Bovine Viral
Female
Fetal Diseases
Male
Prevalence
Foetal infections with bovine viral diarrhoea virus in argentina
topic_facet Argentina
BVDV
Foetal infections
Pestivirus
Prevalence
animal
animal disease
Argentina
article
Bovine diarrhea virus
cattle
cattle disease
female
fetus disease
isolation and purification
male
prevalence
virology
Animals
Argentina
Bovine Virus Diarrhea-Mucosal Disease
Cattle
Diarrhea Viruses, Bovine Viral
Female
Fetal Diseases
Male
Prevalence
description The frequency of isolation of bovine viral diarrhoea virus (BVDV) from primary tissue cultures and organs from bovine foetuses was studied between 1992 and 1994. Around 25% of primary tissue cultures were BVDV positive. Primär)' testis cultures were inoculated with homogenates of spleen, kidney, lung and liver from 52 foetuses. Cells were passaged twice and BVDV antigen investigated by indirect immunofluorescence. Non-cytopathic BVDV was detected in at least one organ in 11/52 foetuses (21.2%): 6/10 spleens, 4/7 kidneys, 7/9 lungs and 3/5 livers. Cytopathic BVDV was detected in lung and kidney from two foetuses. Since only gamma-irradiated sera are used in the laboratory and only inactivated BVDV vaccines are applied in Argentina, it was concluded that these isolations represented field infections. In addition to the 11 virus positive foetuses, two foetuses were positive for BVDV antibodies, which suggested a 25% prevalence of infection. These results stress the need for disease control on a herd basis and the requirement for biological reagents of bovine origin for the detection of BVDV. © 199GBailIièreTindall.
title Foetal infections with bovine viral diarrhoea virus in argentina
title_short Foetal infections with bovine viral diarrhoea virus in argentina
title_full Foetal infections with bovine viral diarrhoea virus in argentina
title_fullStr Foetal infections with bovine viral diarrhoea virus in argentina
title_full_unstemmed Foetal infections with bovine viral diarrhoea virus in argentina
title_sort foetal infections with bovine viral diarrhoea virus in argentina
publishDate 1996
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00071935_v152_n2_p175_Munoz
http://hdl.handle.net/20.500.12110/paper_00071935_v152_n2_p175_Munoz
_version_ 1768545719873961984