Permanent alteration of muscarinic acetylcholine receptor binding in rat striatum after circling training during development
We evaluated the effect of circling training (CT) in the expression of muscarinic acetylcholine receptor (mAchR) in developing rat striatum. For this, male and female rats were subjected to CT at 20, 30, 40 and 60 days of age during 7 days. Animals trained at 30 days but not at other ages showed an...
Guardado en:
| Autores principales: | , , |
|---|---|
| Publicado: |
1995
|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00068993_v705_n1-2_p39_Ibarra http://hdl.handle.net/20.500.12110/paper_00068993_v705_n1-2_p39_Ibarra |
| Aporte de: |
| id |
paper:paper_00068993_v705_n1-2_p39_Ibarra |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_00068993_v705_n1-2_p39_Ibarra2023-06-08T14:31:31Z Permanent alteration of muscarinic acetylcholine receptor binding in rat striatum after circling training during development Rodríguez, Jorge Andrés Paratcha, Gustavo Christian Azcurra, Julio Marcos Circling training Development Muscarinic receptor Plasticity Rat striatum Receptor binding muscarinic receptor quinuclidinyl benzilate adolescent animal experiment article brain development circling behavior controlled study female male nerve cell plasticity nonhuman priority journal rat receptor binding sex difference training Age Factors Animals Behavior, Animal Brain Female Male Motor Activity Neostriatum Rats Rats, Sprague-Dawley Receptors, Muscarinic Sex Factors Time Factors We evaluated the effect of circling training (CT) in the expression of muscarinic acetylcholine receptor (mAchR) in developing rat striatum. For this, male and female rats were subjected to CT at 20, 30, 40 and 60 days of age during 7 days. Animals trained at 30 days but not at other ages showed an average decreased binding to mAchR of 33% in males and 24% in females, representing a significant difference with respect to control non-trained animals (males P < 0.001, females P < 0.005), and showing also a differential response between sex (P < 0.01). mAchR drop was found invariably either 2 months or 1 year after training indicating a long term plastic change due to circling training. Scatchard analysis showed that altered binding represents a variation of the total receptor number instead of its binding affinity, with no significant differences found among Kd (P > 0.1). mAchR variation was correlated with the motor performance accomplished in the test. Regarding total distance run, male rats trained for 3 days (300 meters run), for 5 days (600 meters) and for 7 days (900 meters) showed a drop of 19, 28 and 33% respectively (r2 = 0.91, P < 0.001), while female changes were of 21, 23 and 24% (r2 = 0.78, P < 0.001). Nevertheless, no correlation with running speed was found (r2 = 0.13 male, r2 = 0.02 female; P > 0.1). In summary, these results demonstrate the presence of a limited sensitivity period during striatum development where mAchR expression may be affected by the activity performed during CT, representing a permanent alteration of the receptor levels. © 1995 Elsevier Science B.V. All rights reserved. Fil:Rodriguez, J.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Paratcha, G.Ch. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Azcurra, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1995 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00068993_v705_n1-2_p39_Ibarra http://hdl.handle.net/20.500.12110/paper_00068993_v705_n1-2_p39_Ibarra |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Circling training Development Muscarinic receptor Plasticity Rat striatum Receptor binding muscarinic receptor quinuclidinyl benzilate adolescent animal experiment article brain development circling behavior controlled study female male nerve cell plasticity nonhuman priority journal rat receptor binding sex difference training Age Factors Animals Behavior, Animal Brain Female Male Motor Activity Neostriatum Rats Rats, Sprague-Dawley Receptors, Muscarinic Sex Factors Time Factors |
| spellingShingle |
Circling training Development Muscarinic receptor Plasticity Rat striatum Receptor binding muscarinic receptor quinuclidinyl benzilate adolescent animal experiment article brain development circling behavior controlled study female male nerve cell plasticity nonhuman priority journal rat receptor binding sex difference training Age Factors Animals Behavior, Animal Brain Female Male Motor Activity Neostriatum Rats Rats, Sprague-Dawley Receptors, Muscarinic Sex Factors Time Factors Rodríguez, Jorge Andrés Paratcha, Gustavo Christian Azcurra, Julio Marcos Permanent alteration of muscarinic acetylcholine receptor binding in rat striatum after circling training during development |
| topic_facet |
Circling training Development Muscarinic receptor Plasticity Rat striatum Receptor binding muscarinic receptor quinuclidinyl benzilate adolescent animal experiment article brain development circling behavior controlled study female male nerve cell plasticity nonhuman priority journal rat receptor binding sex difference training Age Factors Animals Behavior, Animal Brain Female Male Motor Activity Neostriatum Rats Rats, Sprague-Dawley Receptors, Muscarinic Sex Factors Time Factors |
| description |
We evaluated the effect of circling training (CT) in the expression of muscarinic acetylcholine receptor (mAchR) in developing rat striatum. For this, male and female rats were subjected to CT at 20, 30, 40 and 60 days of age during 7 days. Animals trained at 30 days but not at other ages showed an average decreased binding to mAchR of 33% in males and 24% in females, representing a significant difference with respect to control non-trained animals (males P < 0.001, females P < 0.005), and showing also a differential response between sex (P < 0.01). mAchR drop was found invariably either 2 months or 1 year after training indicating a long term plastic change due to circling training. Scatchard analysis showed that altered binding represents a variation of the total receptor number instead of its binding affinity, with no significant differences found among Kd (P > 0.1). mAchR variation was correlated with the motor performance accomplished in the test. Regarding total distance run, male rats trained for 3 days (300 meters run), for 5 days (600 meters) and for 7 days (900 meters) showed a drop of 19, 28 and 33% respectively (r2 = 0.91, P < 0.001), while female changes were of 21, 23 and 24% (r2 = 0.78, P < 0.001). Nevertheless, no correlation with running speed was found (r2 = 0.13 male, r2 = 0.02 female; P > 0.1). In summary, these results demonstrate the presence of a limited sensitivity period during striatum development where mAchR expression may be affected by the activity performed during CT, representing a permanent alteration of the receptor levels. © 1995 Elsevier Science B.V. All rights reserved. |
| author |
Rodríguez, Jorge Andrés Paratcha, Gustavo Christian Azcurra, Julio Marcos |
| author_facet |
Rodríguez, Jorge Andrés Paratcha, Gustavo Christian Azcurra, Julio Marcos |
| author_sort |
Rodríguez, Jorge Andrés |
| title |
Permanent alteration of muscarinic acetylcholine receptor binding in rat striatum after circling training during development |
| title_short |
Permanent alteration of muscarinic acetylcholine receptor binding in rat striatum after circling training during development |
| title_full |
Permanent alteration of muscarinic acetylcholine receptor binding in rat striatum after circling training during development |
| title_fullStr |
Permanent alteration of muscarinic acetylcholine receptor binding in rat striatum after circling training during development |
| title_full_unstemmed |
Permanent alteration of muscarinic acetylcholine receptor binding in rat striatum after circling training during development |
| title_sort |
permanent alteration of muscarinic acetylcholine receptor binding in rat striatum after circling training during development |
| publishDate |
1995 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00068993_v705_n1-2_p39_Ibarra http://hdl.handle.net/20.500.12110/paper_00068993_v705_n1-2_p39_Ibarra |
| work_keys_str_mv |
AT rodriguezjorgeandres permanentalterationofmuscarinicacetylcholinereceptorbindinginratstriatumaftercirclingtrainingduringdevelopment AT paratchagustavochristian permanentalterationofmuscarinicacetylcholinereceptorbindinginratstriatumaftercirclingtrainingduringdevelopment AT azcurrajuliomarcos permanentalterationofmuscarinicacetylcholinereceptorbindinginratstriatumaftercirclingtrainingduringdevelopment |
| _version_ |
1768544159674662912 |