Studies on induction of δ-aminolevulinic acid synthase, ferrochelatase, cytochrome P-450 and cyclic AMP by phenformin. Chlorpropamide, allylisopropylacetamide and lead in hepatocytes from normal and experimental diabetic rats

The present work demonstrates that phenformin exerted an inducing effect on δ-amino-levulinic acid synthase (ALA-S) and ferrochelatase activities and on cytochrome P-450 content in isolated hepatocytes from rats with experimental diabetes. Similar results were obtained with respect to ALA-S activity...

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Autores principales: Cánepa, Eduardo Tomás, Llambías, Elena Blanca Cecilia, Grinstein, Moisés
Publicado: 1990
Materias:
5 aminolevulinate synthase
allylisopropylacetamide
bucladesine
chlorpropamide
cyclic amp
cytochrome p450
ferrochelatase
lead acetate
phenformin
phenobarbital
animal cell
animal experiment
animal model
article
cell suspension
drug concentration
enzyme induction
hepatic porphyria
liver cell
male
nonhuman
priority journal
rat
streptozocin diabetes
5-Aminolevulinate Synthetase
Allylisopropylacetamide
Animal
Bucladesine
Chlorpropamide
Cyclic AMP
Cytochrome P-450 Enzyme System
Diabetes Mellitus, Experimental
Enzyme Induction
Ferrochelatase
In Vitro
Lead
Liver
Lyases
Male
Phenformin
Rats
Support, Non-U.S. Gov't
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00062952_v40_n2_p365_Canepa
http://hdl.handle.net/20.500.12110/paper_00062952_v40_n2_p365_Canepa
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_00062952_v40_n2_p365_Canepa
record_format dspace
spelling paper:paper_00062952_v40_n2_p365_Canepa2023-06-08T14:30:33Z Studies on induction of δ-aminolevulinic acid synthase, ferrochelatase, cytochrome P-450 and cyclic AMP by phenformin. Chlorpropamide, allylisopropylacetamide and lead in hepatocytes from normal and experimental diabetic rats Cánepa, Eduardo Tomás Llambías, Elena Blanca Cecilia Grinstein, Moisés 5 aminolevulinate synthase allylisopropylacetamide bucladesine chlorpropamide cyclic amp cytochrome p450 ferrochelatase lead acetate phenformin phenobarbital animal cell animal experiment animal model article cell suspension drug concentration enzyme induction hepatic porphyria liver cell male nonhuman priority journal rat streptozocin diabetes 5-Aminolevulinate Synthetase Allylisopropylacetamide Animal Bucladesine Chlorpropamide Cyclic AMP Cytochrome P-450 Enzyme System Diabetes Mellitus, Experimental Enzyme Induction Ferrochelatase In Vitro Lead Liver Lyases Male Phenformin Rats Support, Non-U.S. Gov't The present work demonstrates that phenformin exerted an inducing effect on δ-amino-levulinic acid synthase (ALA-S) and ferrochelatase activities and on cytochrome P-450 content in isolated hepatocytes from rats with experimental diabetes. Similar results were obtained with respect to ALA-S activity and cytochrome P-450 content when chlorpropamide was used. The inducing effect exerted by allylisopropylacetamide (AIA) on ALA-S and ferrochelatase activities in diabetic hepatic cells was markedly greater than that observed in normal hepatocytes. This stimulatory response was not enhanced by adding dibutyryl cyclic AMP (cAMP). When phenformin was added to isolated rat hepatocytes of normal rats, induction of ALA-S and ferrochelatase activities and cytochrome P-450 content was observed only in the presence of added dibutyryl cAMP. Addition of chlorpropamide to this in vitro system did not exert an inducing effect on the same enzymes even in the presence of dibutyryl cAMP. The present results add more experimental evidence about the lability of the heme pathway of diabetic hepatocytes. © 1990. Fil:Canepa, E.T. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Llambias, E.B.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Grinstein, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1990 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00062952_v40_n2_p365_Canepa http://hdl.handle.net/20.500.12110/paper_00062952_v40_n2_p365_Canepa
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic 5 aminolevulinate synthase
allylisopropylacetamide
bucladesine
chlorpropamide
cyclic amp
cytochrome p450
ferrochelatase
lead acetate
phenformin
phenobarbital
animal cell
animal experiment
animal model
article
cell suspension
drug concentration
enzyme induction
hepatic porphyria
liver cell
male
nonhuman
priority journal
rat
streptozocin diabetes
5-Aminolevulinate Synthetase
Allylisopropylacetamide
Animal
Bucladesine
Chlorpropamide
Cyclic AMP
Cytochrome P-450 Enzyme System
Diabetes Mellitus, Experimental
Enzyme Induction
Ferrochelatase
In Vitro
Lead
Liver
Lyases
Male
Phenformin
Rats
Support, Non-U.S. Gov't
spellingShingle 5 aminolevulinate synthase
allylisopropylacetamide
bucladesine
chlorpropamide
cyclic amp
cytochrome p450
ferrochelatase
lead acetate
phenformin
phenobarbital
animal cell
animal experiment
animal model
article
cell suspension
drug concentration
enzyme induction
hepatic porphyria
liver cell
male
nonhuman
priority journal
rat
streptozocin diabetes
5-Aminolevulinate Synthetase
Allylisopropylacetamide
Animal
Bucladesine
Chlorpropamide
Cyclic AMP
Cytochrome P-450 Enzyme System
Diabetes Mellitus, Experimental
Enzyme Induction
Ferrochelatase
In Vitro
Lead
Liver
Lyases
Male
Phenformin
Rats
Support, Non-U.S. Gov't
Cánepa, Eduardo Tomás
Llambías, Elena Blanca Cecilia
Grinstein, Moisés
Studies on induction of δ-aminolevulinic acid synthase, ferrochelatase, cytochrome P-450 and cyclic AMP by phenformin. Chlorpropamide, allylisopropylacetamide and lead in hepatocytes from normal and experimental diabetic rats
topic_facet 5 aminolevulinate synthase
allylisopropylacetamide
bucladesine
chlorpropamide
cyclic amp
cytochrome p450
ferrochelatase
lead acetate
phenformin
phenobarbital
animal cell
animal experiment
animal model
article
cell suspension
drug concentration
enzyme induction
hepatic porphyria
liver cell
male
nonhuman
priority journal
rat
streptozocin diabetes
5-Aminolevulinate Synthetase
Allylisopropylacetamide
Animal
Bucladesine
Chlorpropamide
Cyclic AMP
Cytochrome P-450 Enzyme System
Diabetes Mellitus, Experimental
Enzyme Induction
Ferrochelatase
In Vitro
Lead
Liver
Lyases
Male
Phenformin
Rats
Support, Non-U.S. Gov't
description The present work demonstrates that phenformin exerted an inducing effect on δ-amino-levulinic acid synthase (ALA-S) and ferrochelatase activities and on cytochrome P-450 content in isolated hepatocytes from rats with experimental diabetes. Similar results were obtained with respect to ALA-S activity and cytochrome P-450 content when chlorpropamide was used. The inducing effect exerted by allylisopropylacetamide (AIA) on ALA-S and ferrochelatase activities in diabetic hepatic cells was markedly greater than that observed in normal hepatocytes. This stimulatory response was not enhanced by adding dibutyryl cyclic AMP (cAMP). When phenformin was added to isolated rat hepatocytes of normal rats, induction of ALA-S and ferrochelatase activities and cytochrome P-450 content was observed only in the presence of added dibutyryl cAMP. Addition of chlorpropamide to this in vitro system did not exert an inducing effect on the same enzymes even in the presence of dibutyryl cAMP. The present results add more experimental evidence about the lability of the heme pathway of diabetic hepatocytes. © 1990.
author Cánepa, Eduardo Tomás
Llambías, Elena Blanca Cecilia
Grinstein, Moisés
author_facet Cánepa, Eduardo Tomás
Llambías, Elena Blanca Cecilia
Grinstein, Moisés
author_sort Cánepa, Eduardo Tomás
title Studies on induction of δ-aminolevulinic acid synthase, ferrochelatase, cytochrome P-450 and cyclic AMP by phenformin. Chlorpropamide, allylisopropylacetamide and lead in hepatocytes from normal and experimental diabetic rats
title_short Studies on induction of δ-aminolevulinic acid synthase, ferrochelatase, cytochrome P-450 and cyclic AMP by phenformin. Chlorpropamide, allylisopropylacetamide and lead in hepatocytes from normal and experimental diabetic rats
title_full Studies on induction of δ-aminolevulinic acid synthase, ferrochelatase, cytochrome P-450 and cyclic AMP by phenformin. Chlorpropamide, allylisopropylacetamide and lead in hepatocytes from normal and experimental diabetic rats
title_fullStr Studies on induction of δ-aminolevulinic acid synthase, ferrochelatase, cytochrome P-450 and cyclic AMP by phenformin. Chlorpropamide, allylisopropylacetamide and lead in hepatocytes from normal and experimental diabetic rats
title_full_unstemmed Studies on induction of δ-aminolevulinic acid synthase, ferrochelatase, cytochrome P-450 and cyclic AMP by phenformin. Chlorpropamide, allylisopropylacetamide and lead in hepatocytes from normal and experimental diabetic rats
title_sort studies on induction of δ-aminolevulinic acid synthase, ferrochelatase, cytochrome p-450 and cyclic amp by phenformin. chlorpropamide, allylisopropylacetamide and lead in hepatocytes from normal and experimental diabetic rats
publishDate 1990
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00062952_v40_n2_p365_Canepa
http://hdl.handle.net/20.500.12110/paper_00062952_v40_n2_p365_Canepa
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AT llambiaselenablancacecilia studiesoninductionofdaminolevulinicacidsynthaseferrochelatasecytochromep450andcyclicampbyphenforminchlorpropamideallylisopropylacetamideandleadinhepatocytesfromnormalandexperimentaldiabeticrats
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