Membrane localization of Junín virus glycoproteins requires cholesterol and cholesterol rich membranes

Arenavirus morphogenesis and budding occurs at cellular plasma membrane; however, the nature of membrane assembly sites remains poorly understood. In this study we examined the effect of different cholesterol-lowering agents on Junín virus (JUNV) multiplication. We found that cholesterol cell deplet...

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Detalles Bibliográficos
Autores principales: Cordo, Sandra M., Valko, Ayelén, Candurra, Nélida Alicia
Publicado: 2013
Materias:
Cholesterol
Junín virus
Membrane rafts
beta cyclodextrin
cholesterol
glycoprotein
triton x 100
virus glycoprotein
virus protein
animal cell
article
controlled study
Junin virus
nonhuman
priority journal
protein localization
virus release
Animals
Anticholesteremic Agents
Cell Membrane
Cercopithecus aethiops
Dogs
Madin Darby Canine Kidney Cells
Membrane Glycoproteins
Vero Cells
Viral Envelope Proteins
Virus Replication
Arenavirus
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v430_n3_p912_Cordo
http://hdl.handle.net/20.500.12110/paper_0006291X_v430_n3_p912_Cordo
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_0006291X_v430_n3_p912_Cordo
record_format dspace
spelling paper:paper_0006291X_v430_n3_p912_Cordo2023-06-08T14:30:21Z Membrane localization of Junín virus glycoproteins requires cholesterol and cholesterol rich membranes Cordo, Sandra M. Valko, Ayelén Candurra, Nélida Alicia Cholesterol Junín virus Membrane rafts beta cyclodextrin cholesterol glycoprotein triton x 100 virus glycoprotein virus protein animal cell article controlled study Junin virus nonhuman priority journal protein localization virus release Animals Anticholesteremic Agents Cell Membrane Cercopithecus aethiops Cholesterol Dogs Junin virus Madin Darby Canine Kidney Cells Membrane Glycoproteins Vero Cells Viral Envelope Proteins Virus Replication Arenavirus Junin virus Arenavirus morphogenesis and budding occurs at cellular plasma membrane; however, the nature of membrane assembly sites remains poorly understood. In this study we examined the effect of different cholesterol-lowering agents on Junín virus (JUNV) multiplication. We found that cholesterol cell depletion reduced JUNV glycoproteins (GPs) membrane expression and virus budding. Analysis of membrane protein insolubility in Triton X-100 suggested that JUNV GPs associate with cholesterol enriched membranes. Rafts dissociation conditions as warm detergent extraction and cholesterol removal by methyl-β-cyclodextrin compound showed to impair GPs cholesterol enriched membrane association. Analysis of GPs transfected cells showed similar results suggesting that membrane raft association is independent of other viral proteins. © 2012 Elsevier Inc. Fil:Cordo, S.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Valko, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Candurra, N.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v430_n3_p912_Cordo http://hdl.handle.net/20.500.12110/paper_0006291X_v430_n3_p912_Cordo
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Cholesterol
Junín virus
Membrane rafts
beta cyclodextrin
cholesterol
glycoprotein
triton x 100
virus glycoprotein
virus protein
animal cell
article
controlled study
Junin virus
nonhuman
priority journal
protein localization
virus release
Animals
Anticholesteremic Agents
Cell Membrane
Cercopithecus aethiops
Cholesterol
Dogs
Junin virus
Madin Darby Canine Kidney Cells
Membrane Glycoproteins
Vero Cells
Viral Envelope Proteins
Virus Replication
Arenavirus
Junin virus
spellingShingle Cholesterol
Junín virus
Membrane rafts
beta cyclodextrin
cholesterol
glycoprotein
triton x 100
virus glycoprotein
virus protein
animal cell
article
controlled study
Junin virus
nonhuman
priority journal
protein localization
virus release
Animals
Anticholesteremic Agents
Cell Membrane
Cercopithecus aethiops
Cholesterol
Dogs
Junin virus
Madin Darby Canine Kidney Cells
Membrane Glycoproteins
Vero Cells
Viral Envelope Proteins
Virus Replication
Arenavirus
Junin virus
Cordo, Sandra M.
Valko, Ayelén
Candurra, Nélida Alicia
Membrane localization of Junín virus glycoproteins requires cholesterol and cholesterol rich membranes
topic_facet Cholesterol
Junín virus
Membrane rafts
beta cyclodextrin
cholesterol
glycoprotein
triton x 100
virus glycoprotein
virus protein
animal cell
article
controlled study
Junin virus
nonhuman
priority journal
protein localization
virus release
Animals
Anticholesteremic Agents
Cell Membrane
Cercopithecus aethiops
Cholesterol
Dogs
Junin virus
Madin Darby Canine Kidney Cells
Membrane Glycoproteins
Vero Cells
Viral Envelope Proteins
Virus Replication
Arenavirus
Junin virus
description Arenavirus morphogenesis and budding occurs at cellular plasma membrane; however, the nature of membrane assembly sites remains poorly understood. In this study we examined the effect of different cholesterol-lowering agents on Junín virus (JUNV) multiplication. We found that cholesterol cell depletion reduced JUNV glycoproteins (GPs) membrane expression and virus budding. Analysis of membrane protein insolubility in Triton X-100 suggested that JUNV GPs associate with cholesterol enriched membranes. Rafts dissociation conditions as warm detergent extraction and cholesterol removal by methyl-β-cyclodextrin compound showed to impair GPs cholesterol enriched membrane association. Analysis of GPs transfected cells showed similar results suggesting that membrane raft association is independent of other viral proteins. © 2012 Elsevier Inc.
author Cordo, Sandra M.
Valko, Ayelén
Candurra, Nélida Alicia
author_facet Cordo, Sandra M.
Valko, Ayelén
Candurra, Nélida Alicia
author_sort Cordo, Sandra M.
title Membrane localization of Junín virus glycoproteins requires cholesterol and cholesterol rich membranes
title_short Membrane localization of Junín virus glycoproteins requires cholesterol and cholesterol rich membranes
title_full Membrane localization of Junín virus glycoproteins requires cholesterol and cholesterol rich membranes
title_fullStr Membrane localization of Junín virus glycoproteins requires cholesterol and cholesterol rich membranes
title_full_unstemmed Membrane localization of Junín virus glycoproteins requires cholesterol and cholesterol rich membranes
title_sort membrane localization of junín virus glycoproteins requires cholesterol and cholesterol rich membranes
publishDate 2013
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v430_n3_p912_Cordo
http://hdl.handle.net/20.500.12110/paper_0006291X_v430_n3_p912_Cordo
work_keys_str_mv AT cordosandram membranelocalizationofjuninvirusglycoproteinsrequirescholesterolandcholesterolrichmembranes
AT valkoayelen membranelocalizationofjuninvirusglycoproteinsrequirescholesterolandcholesterolrichmembranes
AT candurranelidaalicia membranelocalizationofjuninvirusglycoproteinsrequirescholesterolandcholesterolrichmembranes
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