Size-controlled nanopores in lipid membranes with stabilizing electric fields
Molecular dynamics (MD) has been shown to be a useful tool for unveiling many aspects of pore formation in lipid membranes under the influence of an applied electric field. However, the study of the structure and transport properties of electropores by means of MD has been hampered by difficulties i...
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2012
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v423_n2_p325_Fernandez http://hdl.handle.net/20.500.12110/paper_0006291X_v423_n2_p325_Fernandez |
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paper:paper_0006291X_v423_n2_p325_Fernandez2023-06-08T14:30:20Z Size-controlled nanopores in lipid membranes with stabilizing electric fields Fernández, María Laura Electric field Electroporation Lipid membrane Molecular dynamics Stable size-controlled Pores article channel gating controlled study electric field limit of quantitation molecular model nanopore phospholipid bilayer priority journal simulation Electricity Electromagnetic Fields Lipid Bilayers Molecular Dynamics Simulation Nanopores Molecular dynamics (MD) has been shown to be a useful tool for unveiling many aspects of pore formation in lipid membranes under the influence of an applied electric field. However, the study of the structure and transport properties of electropores by means of MD has been hampered by difficulties in the maintenance of a stable electropore in the typically small simulated membrane patches. We describe a new simulation scheme in which an initially larger porating field is systematically reduced after pore formation to lower stabilizing values to produce stable, size-controlled electropores, which can then be characterized at the molecular level. A new method allows the three-dimensional modeling of the irregular shape of the pores obtained as well as the quantification of its volume. The size of the pore is a function of the value of the stabilizing field. At lower fields the pore disappears and the membrane recovers its normal shape, although in some cases long-lived, fragmented pores containing unusual lipid orientations in the bilayer are observed. © 2012 Elsevier Inc. Fil:Fernández, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v423_n2_p325_Fernandez http://hdl.handle.net/20.500.12110/paper_0006291X_v423_n2_p325_Fernandez |
| institution |
Universidad de Buenos Aires |
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I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Electric field Electroporation Lipid membrane Molecular dynamics Stable size-controlled Pores article channel gating controlled study electric field limit of quantitation molecular model nanopore phospholipid bilayer priority journal simulation Electricity Electromagnetic Fields Lipid Bilayers Molecular Dynamics Simulation Nanopores |
| spellingShingle |
Electric field Electroporation Lipid membrane Molecular dynamics Stable size-controlled Pores article channel gating controlled study electric field limit of quantitation molecular model nanopore phospholipid bilayer priority journal simulation Electricity Electromagnetic Fields Lipid Bilayers Molecular Dynamics Simulation Nanopores Fernández, María Laura Size-controlled nanopores in lipid membranes with stabilizing electric fields |
| topic_facet |
Electric field Electroporation Lipid membrane Molecular dynamics Stable size-controlled Pores article channel gating controlled study electric field limit of quantitation molecular model nanopore phospholipid bilayer priority journal simulation Electricity Electromagnetic Fields Lipid Bilayers Molecular Dynamics Simulation Nanopores |
| description |
Molecular dynamics (MD) has been shown to be a useful tool for unveiling many aspects of pore formation in lipid membranes under the influence of an applied electric field. However, the study of the structure and transport properties of electropores by means of MD has been hampered by difficulties in the maintenance of a stable electropore in the typically small simulated membrane patches. We describe a new simulation scheme in which an initially larger porating field is systematically reduced after pore formation to lower stabilizing values to produce stable, size-controlled electropores, which can then be characterized at the molecular level. A new method allows the three-dimensional modeling of the irregular shape of the pores obtained as well as the quantification of its volume. The size of the pore is a function of the value of the stabilizing field. At lower fields the pore disappears and the membrane recovers its normal shape, although in some cases long-lived, fragmented pores containing unusual lipid orientations in the bilayer are observed. © 2012 Elsevier Inc. |
| author |
Fernández, María Laura |
| author_facet |
Fernández, María Laura |
| author_sort |
Fernández, María Laura |
| title |
Size-controlled nanopores in lipid membranes with stabilizing electric fields |
| title_short |
Size-controlled nanopores in lipid membranes with stabilizing electric fields |
| title_full |
Size-controlled nanopores in lipid membranes with stabilizing electric fields |
| title_fullStr |
Size-controlled nanopores in lipid membranes with stabilizing electric fields |
| title_full_unstemmed |
Size-controlled nanopores in lipid membranes with stabilizing electric fields |
| title_sort |
size-controlled nanopores in lipid membranes with stabilizing electric fields |
| publishDate |
2012 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v423_n2_p325_Fernandez http://hdl.handle.net/20.500.12110/paper_0006291X_v423_n2_p325_Fernandez |
| work_keys_str_mv |
AT fernandezmarialaura sizecontrollednanoporesinlipidmembraneswithstabilizingelectricfields |
| _version_ |
1768546519080763392 |