The time-course of cyclic AMP signaling is critical for leukemia U-937 cell differentiation

The regulation of the cAMP signaling is intimately involved in several cellular processes, including cell differentiation. Here, we provide strong evidence supporting that the time-course of cAMP signal is critical for leukemia U-937 cell differentiation. Three stimulating-cAMP agents were used to a...

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Autores principales:	Shayo, Carina C., Fernández, Natalia C.
Publicado:	2004
Materias:	cAMP signaling Cell differentiation Leukemia amthamine calcium complement component C5a cyclic AMP forskolin histamine agonist histamine H2 receptor Myc protein prostaglandin E receptor prostaglandin E2 prostaglandin receptor stimulating agent protein c fos recombinant protein thiazole derivative article biosynthesis cell differentiation cell division cell maturation cell strain U937 controlled study correlation analysis desensitization dose response drug effect enzyme activity genetics human human cell kinetics leukemia cell metabolism physiology priority journal sequence homology signal transduction time
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v314_n3_p798_Shayo http://hdl.handle.net/20.500.12110/paper_0006291X_v314_n3_p798_Shayo
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_0006291X_v314_n3_p798_Shayo
record_format	dspace
spelling	paper:paper_0006291X_v314_n3_p798_Shayo2023-06-08T14:30:14Z The time-course of cyclic AMP signaling is critical for leukemia U-937 cell differentiation Shayo, Carina C. Fernández, Natalia C. cAMP signaling Cell differentiation Leukemia amthamine calcium complement component C5a cyclic AMP forskolin histamine agonist histamine H2 receptor Myc protein prostaglandin E receptor prostaglandin E2 prostaglandin receptor stimulating agent protein c fos recombinant protein thiazole derivative article biosynthesis cell differentiation cell division cell maturation cell strain U937 controlled study correlation analysis desensitization dose response drug effect enzyme activity genetics human human cell kinetics leukemia cell metabolism physiology priority journal sequence homology signal transduction time The regulation of the cAMP signaling is intimately involved in several cellular processes, including cell differentiation. Here, we provide strong evidence supporting that the time-course of cAMP signal is critical for leukemia U-937 cell differentiation. Three stimulating-cAMP agents were used to analyze the correlation between cAMP time-course and cell differentiation. All three agents denoted similar cAMP maximal responses in dose-response experiments. The kinetic of desensitization showed differential characteristics, while H2 receptor desensitized homologously without affecting PGE2 or forskolin effect, PGE2 response showed mixed desensitization characterized by a homologous initial phase followed by a heterologous phase. Regarding forskolin, long-term stimuli attenuated PGE2 and H2 agonist response without affecting adenylyl cyclase activity. In the absence of phosphodiesterase inhibitors, the three agents induced similar maximal cAMP levels after 5min, but only that induced by the H2 agonist returned to basal levels. Consistent with this observation, H2 agonist was not able to induce U-937 cell maturation in contrast to PGE2 and forskolin, supporting the importance of time-course signaling in the determination of cell behavior. © 2003 Elsevier Inc. All rights reserved. Fil:Shayo, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fernández, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2004 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v314_n3_p798_Shayo http://hdl.handle.net/20.500.12110/paper_0006291X_v314_n3_p798_Shayo
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	cAMP signaling Cell differentiation Leukemia amthamine calcium complement component C5a cyclic AMP forskolin histamine agonist histamine H2 receptor Myc protein prostaglandin E receptor prostaglandin E2 prostaglandin receptor stimulating agent protein c fos recombinant protein thiazole derivative article biosynthesis cell differentiation cell division cell maturation cell strain U937 controlled study correlation analysis desensitization dose response drug effect enzyme activity genetics human human cell kinetics leukemia cell metabolism physiology priority journal sequence homology signal transduction time
spellingShingle	cAMP signaling Cell differentiation Leukemia amthamine calcium complement component C5a cyclic AMP forskolin histamine agonist histamine H2 receptor Myc protein prostaglandin E receptor prostaglandin E2 prostaglandin receptor stimulating agent protein c fos recombinant protein thiazole derivative article biosynthesis cell differentiation cell division cell maturation cell strain U937 controlled study correlation analysis desensitization dose response drug effect enzyme activity genetics human human cell kinetics leukemia cell metabolism physiology priority journal sequence homology signal transduction time Shayo, Carina C. Fernández, Natalia C. The time-course of cyclic AMP signaling is critical for leukemia U-937 cell differentiation
topic_facet	cAMP signaling Cell differentiation Leukemia amthamine calcium complement component C5a cyclic AMP forskolin histamine agonist histamine H2 receptor Myc protein prostaglandin E receptor prostaglandin E2 prostaglandin receptor stimulating agent protein c fos recombinant protein thiazole derivative article biosynthesis cell differentiation cell division cell maturation cell strain U937 controlled study correlation analysis desensitization dose response drug effect enzyme activity genetics human human cell kinetics leukemia cell metabolism physiology priority journal sequence homology signal transduction time
description	The regulation of the cAMP signaling is intimately involved in several cellular processes, including cell differentiation. Here, we provide strong evidence supporting that the time-course of cAMP signal is critical for leukemia U-937 cell differentiation. Three stimulating-cAMP agents were used to analyze the correlation between cAMP time-course and cell differentiation. All three agents denoted similar cAMP maximal responses in dose-response experiments. The kinetic of desensitization showed differential characteristics, while H2 receptor desensitized homologously without affecting PGE2 or forskolin effect, PGE2 response showed mixed desensitization characterized by a homologous initial phase followed by a heterologous phase. Regarding forskolin, long-term stimuli attenuated PGE2 and H2 agonist response without affecting adenylyl cyclase activity. In the absence of phosphodiesterase inhibitors, the three agents induced similar maximal cAMP levels after 5min, but only that induced by the H2 agonist returned to basal levels. Consistent with this observation, H2 agonist was not able to induce U-937 cell maturation in contrast to PGE2 and forskolin, supporting the importance of time-course signaling in the determination of cell behavior. © 2003 Elsevier Inc. All rights reserved.
author	Shayo, Carina C. Fernández, Natalia C.
author_facet	Shayo, Carina C. Fernández, Natalia C.
author_sort	Shayo, Carina C.
title	The time-course of cyclic AMP signaling is critical for leukemia U-937 cell differentiation
title_short	The time-course of cyclic AMP signaling is critical for leukemia U-937 cell differentiation
title_full	The time-course of cyclic AMP signaling is critical for leukemia U-937 cell differentiation
title_fullStr	The time-course of cyclic AMP signaling is critical for leukemia U-937 cell differentiation
title_full_unstemmed	The time-course of cyclic AMP signaling is critical for leukemia U-937 cell differentiation
title_sort	time-course of cyclic amp signaling is critical for leukemia u-937 cell differentiation
publishDate	2004
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v314_n3_p798_Shayo http://hdl.handle.net/20.500.12110/paper_0006291X_v314_n3_p798_Shayo
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The time-course of cyclic AMP signaling is critical for leukemia U-937 cell differentiation

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