Control of Leishmania mexicana proliferation by modulation of polyamine intracellular levels
Repeated treatments of Leishmania mexicana promastigote cultures with α-difluoromethylornithine could not block proliferation when the parasite was grown in a rich medium. Although the irreversible inhibitor of ornithine decarboxylase was able to abolish the enzymatic activity under these conditions...
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1991
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v180_n2_p797_Gonzalez http://hdl.handle.net/20.500.12110/paper_0006291X_v180_n2_p797_Gonzalez |
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paper:paper_0006291X_v180_n2_p797_Gonzalez2023-06-08T14:30:07Z Control of Leishmania mexicana proliferation by modulation of polyamine intracellular levels González, Nélida Susana Sanchez, Cecilia Palmira Algranati, Israel David polyamine article cell level growth regulation leishmania mexicana nonhuman priority journal Animal Eflornithine Kinetics Leishmania mexicana Ornithine Decarboxylase Polyamines Reproduction Support, Non-U.S. Gov't Leishmania mexicana Repeated treatments of Leishmania mexicana promastigote cultures with α-difluoromethylornithine could not block proliferation when the parasite was grown in a rich medium. Although the irreversible inhibitor of ornithine decarboxylase was able to abolish the enzymatic activity under these conditions, polyamine depletion was only partial probably due to the uptake of these substances from the external medium. Conversely, when Leishmania was cultivated in a defined medium essentially free of polyamines, α-difluoromethylornithine was able to decrease the growth rate and proliferation was arrested after several passages in the presence of the drug. Parasite multiplication could be resumed by addition of exogenous polyamines, and a strict correlation between Leishmania promastigote growth and intracellular levels of spermidine was observed. © 1991 Academic Press, Inc. Fil:Gonzalez, N.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Sanchez, C.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Algranati, I.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1991 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v180_n2_p797_Gonzalez http://hdl.handle.net/20.500.12110/paper_0006291X_v180_n2_p797_Gonzalez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
polyamine article cell level growth regulation leishmania mexicana nonhuman priority journal Animal Eflornithine Kinetics Leishmania mexicana Ornithine Decarboxylase Polyamines Reproduction Support, Non-U.S. Gov't Leishmania mexicana |
| spellingShingle |
polyamine article cell level growth regulation leishmania mexicana nonhuman priority journal Animal Eflornithine Kinetics Leishmania mexicana Ornithine Decarboxylase Polyamines Reproduction Support, Non-U.S. Gov't Leishmania mexicana González, Nélida Susana Sanchez, Cecilia Palmira Algranati, Israel David Control of Leishmania mexicana proliferation by modulation of polyamine intracellular levels |
| topic_facet |
polyamine article cell level growth regulation leishmania mexicana nonhuman priority journal Animal Eflornithine Kinetics Leishmania mexicana Ornithine Decarboxylase Polyamines Reproduction Support, Non-U.S. Gov't Leishmania mexicana |
| description |
Repeated treatments of Leishmania mexicana promastigote cultures with α-difluoromethylornithine could not block proliferation when the parasite was grown in a rich medium. Although the irreversible inhibitor of ornithine decarboxylase was able to abolish the enzymatic activity under these conditions, polyamine depletion was only partial probably due to the uptake of these substances from the external medium. Conversely, when Leishmania was cultivated in a defined medium essentially free of polyamines, α-difluoromethylornithine was able to decrease the growth rate and proliferation was arrested after several passages in the presence of the drug. Parasite multiplication could be resumed by addition of exogenous polyamines, and a strict correlation between Leishmania promastigote growth and intracellular levels of spermidine was observed. © 1991 Academic Press, Inc. |
| author |
González, Nélida Susana Sanchez, Cecilia Palmira Algranati, Israel David |
| author_facet |
González, Nélida Susana Sanchez, Cecilia Palmira Algranati, Israel David |
| author_sort |
González, Nélida Susana |
| title |
Control of Leishmania mexicana proliferation by modulation of polyamine intracellular levels |
| title_short |
Control of Leishmania mexicana proliferation by modulation of polyamine intracellular levels |
| title_full |
Control of Leishmania mexicana proliferation by modulation of polyamine intracellular levels |
| title_fullStr |
Control of Leishmania mexicana proliferation by modulation of polyamine intracellular levels |
| title_full_unstemmed |
Control of Leishmania mexicana proliferation by modulation of polyamine intracellular levels |
| title_sort |
control of leishmania mexicana proliferation by modulation of polyamine intracellular levels |
| publishDate |
1991 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v180_n2_p797_Gonzalez http://hdl.handle.net/20.500.12110/paper_0006291X_v180_n2_p797_Gonzalez |
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AT gonzaleznelidasusana controlofleishmaniamexicanaproliferationbymodulationofpolyamineintracellularlevels AT sanchezceciliapalmira controlofleishmaniamexicanaproliferationbymodulationofpolyamineintracellularlevels AT algranatiisraeldavid controlofleishmaniamexicanaproliferationbymodulationofpolyamineintracellularlevels |
| _version_ |
1768542766843822080 |