Stable ornithine decarboxylase in promastigotes of Leishmania mexicana

Studies on the decarboxylation of ornithine in Leishmania mexicana have shown that this activity corresponds to a true ornithine decarboxylase rather than to an oxidative decarboxylation or aminotransferase reaction, both of which also give rise to the release of CO2. The stoichiometric relationship...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sanchez, Cecilia Palmira, González, Nélida Susana, Algranati, Israel David
Publicado:	1989
Materias:	ornithine decarboxylase leishmania mexicana nonhuman priority journal protein stability protozoon Animal Carboxy-Lyases Cell-Free System Kinetics Leishmania mexicana Ornithine Decarboxylase Protein Denaturation Pyridoxal Phosphate Substrate Specificity Support, Non-U.S. Gov't
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v161_n2_p754_Sanchez http://hdl.handle.net/20.500.12110/paper_0006291X_v161_n2_p754_Sanchez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_0006291X_v161_n2_p754_Sanchez
record_format	dspace
spelling	paper:paper_0006291X_v161_n2_p754_Sanchez2023-06-08T14:30:06Z Stable ornithine decarboxylase in promastigotes of Leishmania mexicana Sanchez, Cecilia Palmira González, Nélida Susana Algranati, Israel David ornithine decarboxylase leishmania mexicana nonhuman priority journal protein stability protozoon Animal Carboxy-Lyases Cell-Free System Kinetics Leishmania mexicana Ornithine Decarboxylase Protein Denaturation Pyridoxal Phosphate Substrate Specificity Support, Non-U.S. Gov't Studies on the decarboxylation of ornithine in Leishmania mexicana have shown that this activity corresponds to a true ornithine decarboxylase rather than to an oxidative decarboxylation or aminotransferase reaction, both of which also give rise to the release of CO2. The stoichiometric relationship between substrate and products has indicated that extracts of L. mexicana were able to catalyse the formation of an unknown compound besides putrescine and CO2. The addition of cycloheximide to cultures of L. mexicana allowed us to demonstrate that ornithine decarboxylase degradation in vivo was extremely slow in this parasite. This remarkable stability of the enzyme is only comparable to that found in Trypanosoma brucei and contrasts with the high turnover rate of ornithine decarboxylases of different mammalian cells. © 1989. Fil:Sanchez, C.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gonzalez, N.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Algranati, I.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1989 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v161_n2_p754_Sanchez http://hdl.handle.net/20.500.12110/paper_0006291X_v161_n2_p754_Sanchez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	ornithine decarboxylase leishmania mexicana nonhuman priority journal protein stability protozoon Animal Carboxy-Lyases Cell-Free System Kinetics Leishmania mexicana Ornithine Decarboxylase Protein Denaturation Pyridoxal Phosphate Substrate Specificity Support, Non-U.S. Gov't
spellingShingle	ornithine decarboxylase leishmania mexicana nonhuman priority journal protein stability protozoon Animal Carboxy-Lyases Cell-Free System Kinetics Leishmania mexicana Ornithine Decarboxylase Protein Denaturation Pyridoxal Phosphate Substrate Specificity Support, Non-U.S. Gov't Sanchez, Cecilia Palmira González, Nélida Susana Algranati, Israel David Stable ornithine decarboxylase in promastigotes of Leishmania mexicana
topic_facet	ornithine decarboxylase leishmania mexicana nonhuman priority journal protein stability protozoon Animal Carboxy-Lyases Cell-Free System Kinetics Leishmania mexicana Ornithine Decarboxylase Protein Denaturation Pyridoxal Phosphate Substrate Specificity Support, Non-U.S. Gov't
description	Studies on the decarboxylation of ornithine in Leishmania mexicana have shown that this activity corresponds to a true ornithine decarboxylase rather than to an oxidative decarboxylation or aminotransferase reaction, both of which also give rise to the release of CO2. The stoichiometric relationship between substrate and products has indicated that extracts of L. mexicana were able to catalyse the formation of an unknown compound besides putrescine and CO2. The addition of cycloheximide to cultures of L. mexicana allowed us to demonstrate that ornithine decarboxylase degradation in vivo was extremely slow in this parasite. This remarkable stability of the enzyme is only comparable to that found in Trypanosoma brucei and contrasts with the high turnover rate of ornithine decarboxylases of different mammalian cells. © 1989.
author	Sanchez, Cecilia Palmira González, Nélida Susana Algranati, Israel David
author_facet	Sanchez, Cecilia Palmira González, Nélida Susana Algranati, Israel David
author_sort	Sanchez, Cecilia Palmira
title	Stable ornithine decarboxylase in promastigotes of Leishmania mexicana
title_short	Stable ornithine decarboxylase in promastigotes of Leishmania mexicana
title_full	Stable ornithine decarboxylase in promastigotes of Leishmania mexicana
title_fullStr	Stable ornithine decarboxylase in promastigotes of Leishmania mexicana
title_full_unstemmed	Stable ornithine decarboxylase in promastigotes of Leishmania mexicana
title_sort	stable ornithine decarboxylase in promastigotes of leishmania mexicana
publishDate	1989
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v161_n2_p754_Sanchez http://hdl.handle.net/20.500.12110/paper_0006291X_v161_n2_p754_Sanchez
work_keys_str_mv	AT sanchezceciliapalmira stableornithinedecarboxylaseinpromastigotesofleishmaniamexicana AT gonzaleznelidasusana stableornithinedecarboxylaseinpromastigotesofleishmaniamexicana AT algranatiisraeldavid stableornithinedecarboxylaseinpromastigotesofleishmaniamexicana
_version_	1768544436931788800

Stable ornithine decarboxylase in promastigotes of Leishmania mexicana

Ejemplares similares