In vitro synthesis of a lipid-linked acetylated and pyruvilated olicosaccharide in Rhizobium trifolii

EDTA-treated Rhizobium trifolii cells (strain NA30) incorporate radioactivity from (14C) labeled UDP-Clc, UDP-ClcA, Acetyl-Coa and/or phosphoenol pyruvate into chloroform:methanol:water (1:2:0.3) extracts. The incorporation products have properties of prenyl-phospho-sugars; mild alkaline hydrolysis...

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Autores principales:	Bossio, Juan Carlos, Dankert, Marcelo Alberto
Publicado:	1986
Materias:	acetic acid lipid oligosaccharide pyruvic acid nonhuman priority journal rhizobium trifolii Acetylation Carbohydrate Sequence Chromatography, Gel Electrophoresis, Paper Lipids Oligosaccharides Pyruvates Pyruvic Acid Rhizobium Support, Non-U.S. Gov't
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v134_n1_p205_Bossio http://hdl.handle.net/20.500.12110/paper_0006291X_v134_n1_p205_Bossio
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_0006291X_v134_n1_p205_Bossio
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spelling	paper:paper_0006291X_v134_n1_p205_Bossio2023-06-08T14:30:03Z In vitro synthesis of a lipid-linked acetylated and pyruvilated olicosaccharide in Rhizobium trifolii Bossio, Juan Carlos Dankert, Marcelo Alberto acetic acid lipid oligosaccharide pyruvic acid nonhuman priority journal rhizobium trifolii Acetylation Carbohydrate Sequence Chromatography, Gel Electrophoresis, Paper Lipids Oligosaccharides Pyruvates Pyruvic Acid Rhizobium Support, Non-U.S. Gov't EDTA-treated Rhizobium trifolii cells (strain NA30) incorporate radioactivity from (14C) labeled UDP-Clc, UDP-ClcA, Acetyl-Coa and/or phosphoenol pyruvate into chloroform:methanol:water (1:2:0.3) extracts. The incorporation products have properties of prenyl-phospho-sugars; mild alkaline hydrolysis of these extracts produce cyclic phosphate esters suggesting the presence of a diphosphate bridge, and mild acid or catalytic reduction-alkaline phosphatase treatments release four main componenets a, b, c and d, as judged by paper electrophoresis and chromatography and gel filtration studies. The four components can be obtained (14C)acetyl-labeled, but only compound c and to a lesser degree compound b can be (14C)pyruvate-labeled. For the exopolysaccharide produced by this strain the following repeating unit has been proposed (Robertsen et al. (1981), Plant Physiol. 67, 389-400): {A figure is presented}. The results obtained suggest that the octasaccharide repeating unit (compound a) with one (compound b) or two (compound c) ketal pyruvate residues are assembled on a lipid acceptor. All these compounds are assumed to be intermediates in the biosynthesis of R. trifolii exopolysaccharide. © 1986. Fil:Bossio, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Dankert, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1986 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v134_n1_p205_Bossio http://hdl.handle.net/20.500.12110/paper_0006291X_v134_n1_p205_Bossio
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	acetic acid lipid oligosaccharide pyruvic acid nonhuman priority journal rhizobium trifolii Acetylation Carbohydrate Sequence Chromatography, Gel Electrophoresis, Paper Lipids Oligosaccharides Pyruvates Pyruvic Acid Rhizobium Support, Non-U.S. Gov't
spellingShingle	acetic acid lipid oligosaccharide pyruvic acid nonhuman priority journal rhizobium trifolii Acetylation Carbohydrate Sequence Chromatography, Gel Electrophoresis, Paper Lipids Oligosaccharides Pyruvates Pyruvic Acid Rhizobium Support, Non-U.S. Gov't Bossio, Juan Carlos Dankert, Marcelo Alberto In vitro synthesis of a lipid-linked acetylated and pyruvilated olicosaccharide in Rhizobium trifolii
topic_facet	acetic acid lipid oligosaccharide pyruvic acid nonhuman priority journal rhizobium trifolii Acetylation Carbohydrate Sequence Chromatography, Gel Electrophoresis, Paper Lipids Oligosaccharides Pyruvates Pyruvic Acid Rhizobium Support, Non-U.S. Gov't
description	EDTA-treated Rhizobium trifolii cells (strain NA30) incorporate radioactivity from (14C) labeled UDP-Clc, UDP-ClcA, Acetyl-Coa and/or phosphoenol pyruvate into chloroform:methanol:water (1:2:0.3) extracts. The incorporation products have properties of prenyl-phospho-sugars; mild alkaline hydrolysis of these extracts produce cyclic phosphate esters suggesting the presence of a diphosphate bridge, and mild acid or catalytic reduction-alkaline phosphatase treatments release four main componenets a, b, c and d, as judged by paper electrophoresis and chromatography and gel filtration studies. The four components can be obtained (14C)acetyl-labeled, but only compound c and to a lesser degree compound b can be (14C)pyruvate-labeled. For the exopolysaccharide produced by this strain the following repeating unit has been proposed (Robertsen et al. (1981), Plant Physiol. 67, 389-400): {A figure is presented}. The results obtained suggest that the octasaccharide repeating unit (compound a) with one (compound b) or two (compound c) ketal pyruvate residues are assembled on a lipid acceptor. All these compounds are assumed to be intermediates in the biosynthesis of R. trifolii exopolysaccharide. © 1986.
author	Bossio, Juan Carlos Dankert, Marcelo Alberto
author_facet	Bossio, Juan Carlos Dankert, Marcelo Alberto
author_sort	Bossio, Juan Carlos
title	In vitro synthesis of a lipid-linked acetylated and pyruvilated olicosaccharide in Rhizobium trifolii
title_short	In vitro synthesis of a lipid-linked acetylated and pyruvilated olicosaccharide in Rhizobium trifolii
title_full	In vitro synthesis of a lipid-linked acetylated and pyruvilated olicosaccharide in Rhizobium trifolii
title_fullStr	In vitro synthesis of a lipid-linked acetylated and pyruvilated olicosaccharide in Rhizobium trifolii
title_full_unstemmed	In vitro synthesis of a lipid-linked acetylated and pyruvilated olicosaccharide in Rhizobium trifolii
title_sort	in vitro synthesis of a lipid-linked acetylated and pyruvilated olicosaccharide in rhizobium trifolii
publishDate	1986
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v134_n1_p205_Bossio http://hdl.handle.net/20.500.12110/paper_0006291X_v134_n1_p205_Bossio
work_keys_str_mv	AT bossiojuancarlos invitrosynthesisofalipidlinkedacetylatedandpyruvilatedolicosaccharideinrhizobiumtrifolii AT dankertmarceloalberto invitrosynthesisofalipidlinkedacetylatedandpyruvilatedolicosaccharideinrhizobiumtrifolii
_version_	1768544848103604224

In vitro synthesis of a lipid-linked acetylated and pyruvilated olicosaccharide in Rhizobium trifolii

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