An intermediate in cyclic β1-2 glucan biosynthesis

Incubation of UDP-[14C]Glc with the inner membranes of Agrobacterium tumefaciens leads to the formation of cyclic β1-2 glucan and trichloroacetic acid-insoluble compounds. The proteolysis products of the latter show a positive charge in acid and a negative charge in alkaline buffers. The cyclic β1-2...

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Autores principales:	Zorreguieta, Angeles, Ugalde, Rodolfo Augusto
Publicado:	1985
Materias:	radioisotope agrobacterium tumefaciens beta 1,2 glucan nonhuman priority journal uridine diphosphate glucose c 14 Agrobacterium Chromatography, Paper Electrophoresis, Polyacrylamide Gel Glucans Hydrogen-Ion Concentration Models, Chemical
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v126_n1_p352_Zorreguieta http://hdl.handle.net/20.500.12110/paper_0006291X_v126_n1_p352_Zorreguieta
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_0006291X_v126_n1_p352_Zorreguieta
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spelling	paper:paper_0006291X_v126_n1_p352_Zorreguieta2023-06-08T14:30:02Z An intermediate in cyclic β1-2 glucan biosynthesis Zorreguieta, Angeles Ugalde, Rodolfo Augusto radioisotope agrobacterium tumefaciens beta 1,2 glucan nonhuman priority journal uridine diphosphate glucose c 14 Agrobacterium Chromatography, Paper Electrophoresis, Polyacrylamide Gel Glucans Hydrogen-Ion Concentration Models, Chemical Incubation of UDP-[14C]Glc with the inner membranes of Agrobacterium tumefaciens leads to the formation of cyclic β1-2 glucan and trichloroacetic acid-insoluble compounds. The proteolysis products of the latter show a positive charge in acid and a negative charge in alkaline buffers. The cyclic β1-2 glucan and the trichloroacetic acid insoluble compounds yield the same products on partial acid hydrolysis. Addition of excess non-radioactive UDP-Glc to the reaction mixture nearly stops the formation of radioactive β1-2 glucan and leads to a rapid fall of radioactivity in the trichloroacetic acid precipitate. Alkaline treatment of the insoluble compounds under conditions of β-elimination leads to the partial release of free saccharides (about 30%). It is concluded that β1-2 glucan chains are built up joined to a protein and then released as free cyclic β1-2 glucan. © 1985. Fil:Zorreguieta, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ugalde, R.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1985 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v126_n1_p352_Zorreguieta http://hdl.handle.net/20.500.12110/paper_0006291X_v126_n1_p352_Zorreguieta
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	radioisotope agrobacterium tumefaciens beta 1,2 glucan nonhuman priority journal uridine diphosphate glucose c 14 Agrobacterium Chromatography, Paper Electrophoresis, Polyacrylamide Gel Glucans Hydrogen-Ion Concentration Models, Chemical
spellingShingle	radioisotope agrobacterium tumefaciens beta 1,2 glucan nonhuman priority journal uridine diphosphate glucose c 14 Agrobacterium Chromatography, Paper Electrophoresis, Polyacrylamide Gel Glucans Hydrogen-Ion Concentration Models, Chemical Zorreguieta, Angeles Ugalde, Rodolfo Augusto An intermediate in cyclic β1-2 glucan biosynthesis
topic_facet	radioisotope agrobacterium tumefaciens beta 1,2 glucan nonhuman priority journal uridine diphosphate glucose c 14 Agrobacterium Chromatography, Paper Electrophoresis, Polyacrylamide Gel Glucans Hydrogen-Ion Concentration Models, Chemical
description	Incubation of UDP-[14C]Glc with the inner membranes of Agrobacterium tumefaciens leads to the formation of cyclic β1-2 glucan and trichloroacetic acid-insoluble compounds. The proteolysis products of the latter show a positive charge in acid and a negative charge in alkaline buffers. The cyclic β1-2 glucan and the trichloroacetic acid insoluble compounds yield the same products on partial acid hydrolysis. Addition of excess non-radioactive UDP-Glc to the reaction mixture nearly stops the formation of radioactive β1-2 glucan and leads to a rapid fall of radioactivity in the trichloroacetic acid precipitate. Alkaline treatment of the insoluble compounds under conditions of β-elimination leads to the partial release of free saccharides (about 30%). It is concluded that β1-2 glucan chains are built up joined to a protein and then released as free cyclic β1-2 glucan. © 1985.
author	Zorreguieta, Angeles Ugalde, Rodolfo Augusto
author_facet	Zorreguieta, Angeles Ugalde, Rodolfo Augusto
author_sort	Zorreguieta, Angeles
title	An intermediate in cyclic β1-2 glucan biosynthesis
title_short	An intermediate in cyclic β1-2 glucan biosynthesis
title_full	An intermediate in cyclic β1-2 glucan biosynthesis
title_fullStr	An intermediate in cyclic β1-2 glucan biosynthesis
title_full_unstemmed	An intermediate in cyclic β1-2 glucan biosynthesis
title_sort	intermediate in cyclic β1-2 glucan biosynthesis
publishDate	1985
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v126_n1_p352_Zorreguieta http://hdl.handle.net/20.500.12110/paper_0006291X_v126_n1_p352_Zorreguieta
work_keys_str_mv	AT zorreguietaangeles anintermediateincyclicb12glucanbiosynthesis AT ugalderodolfoaugusto anintermediateincyclicb12glucanbiosynthesis AT zorreguietaangeles intermediateincyclicb12glucanbiosynthesis AT ugalderodolfoaugusto intermediateincyclicb12glucanbiosynthesis
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An intermediate in cyclic β1-2 glucan biosynthesis

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