Initiation, elongation and termination of polypeptide synthesis in cell-free systems from polyamine-deficient bacteria
Protein synthesis in a polyamine-auxotrophic mutant of E. coli cultivated in the absence and presence of putrescine has been studied. The ratio of in vivo polypeptide synthesis under both conditions was the same as that obtained with cell-free extracts derived from polyamine-depleted and supplemente...
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1981
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v103_n1_p8_Algranati http://hdl.handle.net/20.500.12110/paper_0006291X_v103_n1_p8_Algranati |
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paper:paper_0006291X_v103_n1_p8_Algranati2023-06-08T14:30:00Z Initiation, elongation and termination of polypeptide synthesis in cell-free systems from polyamine-deficient bacteria Algranati, Israel David Goldemberg, Sara Hebe putrescine spermidine spermine animal experiment auxotrophic mutant escherichia coli heredity in vitro study protein synthesis Cell-Free System Escherichia coli Kinetics Mutation Peptide Chain Elongation Peptide Chain Initiation Peptide Chain Termination Putrescine Ribosomes RNA, Transfer, Amino Acyl Support, Non-U.S. Gov't Protein synthesis in a polyamine-auxotrophic mutant of E. coli cultivated in the absence and presence of putrescine has been studied. The ratio of in vivo polypeptide synthesis under both conditions was the same as that obtained with cell-free extracts derived from polyamine-depleted and supplemented bacteria. The markedly reduced activity in systems from polyamine starved cells is most probably due to a poorly efficient initiation step. The estimation of nascent peptide chains and their release from tRNA support the idea that polypeptide elongation and termination processes do not have decreased rates in systems from polyamine-depleted bacteria. © 1981. Fil:Algranati, I.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Goldemberg, S.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1981 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v103_n1_p8_Algranati http://hdl.handle.net/20.500.12110/paper_0006291X_v103_n1_p8_Algranati |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
putrescine spermidine spermine animal experiment auxotrophic mutant escherichia coli heredity in vitro study protein synthesis Cell-Free System Escherichia coli Kinetics Mutation Peptide Chain Elongation Peptide Chain Initiation Peptide Chain Termination Putrescine Ribosomes RNA, Transfer, Amino Acyl Support, Non-U.S. Gov't |
| spellingShingle |
putrescine spermidine spermine animal experiment auxotrophic mutant escherichia coli heredity in vitro study protein synthesis Cell-Free System Escherichia coli Kinetics Mutation Peptide Chain Elongation Peptide Chain Initiation Peptide Chain Termination Putrescine Ribosomes RNA, Transfer, Amino Acyl Support, Non-U.S. Gov't Algranati, Israel David Goldemberg, Sara Hebe Initiation, elongation and termination of polypeptide synthesis in cell-free systems from polyamine-deficient bacteria |
| topic_facet |
putrescine spermidine spermine animal experiment auxotrophic mutant escherichia coli heredity in vitro study protein synthesis Cell-Free System Escherichia coli Kinetics Mutation Peptide Chain Elongation Peptide Chain Initiation Peptide Chain Termination Putrescine Ribosomes RNA, Transfer, Amino Acyl Support, Non-U.S. Gov't |
| description |
Protein synthesis in a polyamine-auxotrophic mutant of E. coli cultivated in the absence and presence of putrescine has been studied. The ratio of in vivo polypeptide synthesis under both conditions was the same as that obtained with cell-free extracts derived from polyamine-depleted and supplemented bacteria. The markedly reduced activity in systems from polyamine starved cells is most probably due to a poorly efficient initiation step. The estimation of nascent peptide chains and their release from tRNA support the idea that polypeptide elongation and termination processes do not have decreased rates in systems from polyamine-depleted bacteria. © 1981. |
| author |
Algranati, Israel David Goldemberg, Sara Hebe |
| author_facet |
Algranati, Israel David Goldemberg, Sara Hebe |
| author_sort |
Algranati, Israel David |
| title |
Initiation, elongation and termination of polypeptide synthesis in cell-free systems from polyamine-deficient bacteria |
| title_short |
Initiation, elongation and termination of polypeptide synthesis in cell-free systems from polyamine-deficient bacteria |
| title_full |
Initiation, elongation and termination of polypeptide synthesis in cell-free systems from polyamine-deficient bacteria |
| title_fullStr |
Initiation, elongation and termination of polypeptide synthesis in cell-free systems from polyamine-deficient bacteria |
| title_full_unstemmed |
Initiation, elongation and termination of polypeptide synthesis in cell-free systems from polyamine-deficient bacteria |
| title_sort |
initiation, elongation and termination of polypeptide synthesis in cell-free systems from polyamine-deficient bacteria |
| publishDate |
1981 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v103_n1_p8_Algranati http://hdl.handle.net/20.500.12110/paper_0006291X_v103_n1_p8_Algranati |
| work_keys_str_mv |
AT algranatiisraeldavid initiationelongationandterminationofpolypeptidesynthesisincellfreesystemsfrompolyaminedeficientbacteria AT goldembergsarahebe initiationelongationandterminationofpolypeptidesynthesisincellfreesystemsfrompolyaminedeficientbacteria |
| _version_ |
1768541544279703552 |