Variations in the levels of cyclic adenosine 3′:5′-monophosphate and in the activities of adenylate cyclase and cyclic adenosine 3t: ́5′-monophosphate phosphodiesterase during aerobic morphogenesis of Mucor rouxii

Particulate cell fractions of mycelium of Mucor rouxii contain adenylate cyclase activity which can be partially solubilized by 2% Lubrol PX. The enzyme requires Mn2+ and its activity is not modified by NaF or guanosine nucleotides. Mycelial extracts also contain cyclic adenosine 3′:5′-monophosphate...

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Autores principales:	Cantore de Ezcurra, María Leonor, Galvagno, Miguel Angel
Publicado:	1980
Materias:	3',5' cyclic nucleotide phosphodiesterase adenylate cyclase cyclic AMP manganese aerobic metabolism article cell fractionation fungus spore metabolism morphogenesis Mucor 3',5'-Cyclic-Nucleotide Phosphodiesterase Adenylate Cyclase Aerobiosis Cyclic AMP Manganese Morphogenesis Spores, Fungal Subcellular Fractions
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v199_n2_p312_Cantore http://hdl.handle.net/20.500.12110/paper_00039861_v199_n2_p312_Cantore
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00039861_v199_n2_p312_Cantore
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spelling	paper:paper_00039861_v199_n2_p312_Cantore2023-06-08T14:24:57Z Variations in the levels of cyclic adenosine 3′:5′-monophosphate and in the activities of adenylate cyclase and cyclic adenosine 3t: ́5′-monophosphate phosphodiesterase during aerobic morphogenesis of Mucor rouxii Cantore de Ezcurra, María Leonor Galvagno, Miguel Angel 3',5' cyclic nucleotide phosphodiesterase adenylate cyclase cyclic AMP manganese aerobic metabolism article cell fractionation fungus spore metabolism morphogenesis Mucor 3',5'-Cyclic-Nucleotide Phosphodiesterase Adenylate Cyclase Aerobiosis Cyclic AMP Manganese Morphogenesis Mucor Spores, Fungal Subcellular Fractions Particulate cell fractions of mycelium of Mucor rouxii contain adenylate cyclase activity which can be partially solubilized by 2% Lubrol PX. The enzyme requires Mn2+ and its activity is not modified by NaF or guanosine nucleotides. Mycelial extracts also contain cyclic adenosine 3′:5′-monophosphate phosphodiesterase activity, 60% of which is soluble. This activity shows characteristic low Km (1 μm) for cyclic AMP and does not hydrolyze cyclic guanosine 3′:5′-monophosphate. It requires Mn2+ ions for maximal activity and is not inhibited by methylxanthines or activated by imidazole. Both enzymatic activities vary during the aerobic life cycle of the fungus. The spores have the highest levels of adenylate cyclase and cAMP phosphodiesterase, which decrease during the aerobic development. At the round cell stage, phosphodiesterase activity reaches 40% of the activity of the spores and varies only slightly thereafter. At this stage the specific activity of adenylate cyclase is 25% of the activity of ungerminated spores, and from this stage on, the activity increases up to the end of the logarithmic phase. Intracellular levels of cyclic AMP have been measured during aerobic germination. The variations of the intracellular level are tentatively explained by unequal variations in the activities of adenylate cyclase and cyclic AMP phosphodiesterase. A continuous increase of the extracellular cyclic AMP level during aerobic development has also been found, which cannot be accounted for solely by variations in the cyclase and diesterase activities. © 1980. Fil:Cantore, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Galvagno, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1980 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v199_n2_p312_Cantore http://hdl.handle.net/20.500.12110/paper_00039861_v199_n2_p312_Cantore
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	3',5' cyclic nucleotide phosphodiesterase adenylate cyclase cyclic AMP manganese aerobic metabolism article cell fractionation fungus spore metabolism morphogenesis Mucor 3',5'-Cyclic-Nucleotide Phosphodiesterase Adenylate Cyclase Aerobiosis Cyclic AMP Manganese Morphogenesis Mucor Spores, Fungal Subcellular Fractions
spellingShingle	3',5' cyclic nucleotide phosphodiesterase adenylate cyclase cyclic AMP manganese aerobic metabolism article cell fractionation fungus spore metabolism morphogenesis Mucor 3',5'-Cyclic-Nucleotide Phosphodiesterase Adenylate Cyclase Aerobiosis Cyclic AMP Manganese Morphogenesis Mucor Spores, Fungal Subcellular Fractions Cantore de Ezcurra, María Leonor Galvagno, Miguel Angel Variations in the levels of cyclic adenosine 3′:5′-monophosphate and in the activities of adenylate cyclase and cyclic adenosine 3t: ́5′-monophosphate phosphodiesterase during aerobic morphogenesis of Mucor rouxii
topic_facet	3',5' cyclic nucleotide phosphodiesterase adenylate cyclase cyclic AMP manganese aerobic metabolism article cell fractionation fungus spore metabolism morphogenesis Mucor 3',5'-Cyclic-Nucleotide Phosphodiesterase Adenylate Cyclase Aerobiosis Cyclic AMP Manganese Morphogenesis Mucor Spores, Fungal Subcellular Fractions
description	Particulate cell fractions of mycelium of Mucor rouxii contain adenylate cyclase activity which can be partially solubilized by 2% Lubrol PX. The enzyme requires Mn2+ and its activity is not modified by NaF or guanosine nucleotides. Mycelial extracts also contain cyclic adenosine 3′:5′-monophosphate phosphodiesterase activity, 60% of which is soluble. This activity shows characteristic low Km (1 μm) for cyclic AMP and does not hydrolyze cyclic guanosine 3′:5′-monophosphate. It requires Mn2+ ions for maximal activity and is not inhibited by methylxanthines or activated by imidazole. Both enzymatic activities vary during the aerobic life cycle of the fungus. The spores have the highest levels of adenylate cyclase and cAMP phosphodiesterase, which decrease during the aerobic development. At the round cell stage, phosphodiesterase activity reaches 40% of the activity of the spores and varies only slightly thereafter. At this stage the specific activity of adenylate cyclase is 25% of the activity of ungerminated spores, and from this stage on, the activity increases up to the end of the logarithmic phase. Intracellular levels of cyclic AMP have been measured during aerobic germination. The variations of the intracellular level are tentatively explained by unequal variations in the activities of adenylate cyclase and cyclic AMP phosphodiesterase. A continuous increase of the extracellular cyclic AMP level during aerobic development has also been found, which cannot be accounted for solely by variations in the cyclase and diesterase activities. © 1980.
author	Cantore de Ezcurra, María Leonor Galvagno, Miguel Angel
author_facet	Cantore de Ezcurra, María Leonor Galvagno, Miguel Angel
author_sort	Cantore de Ezcurra, María Leonor
title	Variations in the levels of cyclic adenosine 3′:5′-monophosphate and in the activities of adenylate cyclase and cyclic adenosine 3t: ́5′-monophosphate phosphodiesterase during aerobic morphogenesis of Mucor rouxii
title_short	Variations in the levels of cyclic adenosine 3′:5′-monophosphate and in the activities of adenylate cyclase and cyclic adenosine 3t: ́5′-monophosphate phosphodiesterase during aerobic morphogenesis of Mucor rouxii
title_full	Variations in the levels of cyclic adenosine 3′:5′-monophosphate and in the activities of adenylate cyclase and cyclic adenosine 3t: ́5′-monophosphate phosphodiesterase during aerobic morphogenesis of Mucor rouxii
title_fullStr	Variations in the levels of cyclic adenosine 3′:5′-monophosphate and in the activities of adenylate cyclase and cyclic adenosine 3t: ́5′-monophosphate phosphodiesterase during aerobic morphogenesis of Mucor rouxii
title_full_unstemmed	Variations in the levels of cyclic adenosine 3′:5′-monophosphate and in the activities of adenylate cyclase and cyclic adenosine 3t: ́5′-monophosphate phosphodiesterase during aerobic morphogenesis of Mucor rouxii
title_sort	variations in the levels of cyclic adenosine 3′:5′-monophosphate and in the activities of adenylate cyclase and cyclic adenosine 3t: ́5′-monophosphate phosphodiesterase during aerobic morphogenesis of mucor rouxii
publishDate	1980
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v199_n2_p312_Cantore http://hdl.handle.net/20.500.12110/paper_00039861_v199_n2_p312_Cantore
work_keys_str_mv	AT cantoredeezcurramarialeonor variationsinthelevelsofcyclicadenosine35monophosphateandintheactivitiesofadenylatecyclaseandcyclicadenosine3t5monophosphatephosphodiesteraseduringaerobicmorphogenesisofmucorrouxii AT galvagnomiguelangel variationsinthelevelsofcyclicadenosine35monophosphateandintheactivitiesofadenylatecyclaseandcyclicadenosine3t5monophosphatephosphodiesteraseduringaerobicmorphogenesisofmucorrouxii
_version_	1768541635330703360

Variations in the levels of cyclic adenosine 3′:5′-monophosphate and in the activities of adenylate cyclase and cyclic adenosine 3t: ́5′-monophosphate phosphodiesterase during aerobic morphogenesis of Mucor rouxii

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