In vitro synthesis of particulate glycogen from uridine diphosphate glucose. III. Some properties of the product synthesized by muscle glycogen synthetase

High molecular weight glycogen has been prepared in vitro with muscle glycogen synthetase (uridine diphosphate glucose: α-1,4-glucan α-4-glucosyltransferase, EC 2.4.1.11) and liver or muscle branching enzyme (α-1,4-glucan: α-1,4-glucan 6-glycosyltransferase, EC 2.4.1.18) with uridine diphosphate glu...

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Autores principales:	Krisman de Fischman, Clara Rebeca, Parodi, Armando José
Publicado:	1970
Materias:	ammonium derivative glucose glucosyltransferase glycogen phosphate pyrimidine nucleotide sodium chloride sulfate animal article biosynthesis enzymology glycogen liver level liver molecular weight muscle rat time Ammonium Compounds Animal Glucose Glucosyltransferases Glycogen Liver Liver Glycogen Molecular Weight Muscles Phosphates Rats Sodium Chloride Sulfates Time Factors Uracil Nucleotides
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v141_n1_p228_Krisman http://hdl.handle.net/20.500.12110/paper_00039861_v141_n1_p228_Krisman
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00039861_v141_n1_p228_Krisman
record_format	dspace
spelling	paper:paper_00039861_v141_n1_p228_Krisman2023-06-08T14:24:55Z In vitro synthesis of particulate glycogen from uridine diphosphate glucose. III. Some properties of the product synthesized by muscle glycogen synthetase Krisman de Fischman, Clara Rebeca Parodi, Armando José ammonium derivative glucose glucosyltransferase glycogen phosphate pyrimidine nucleotide sodium chloride sulfate animal article biosynthesis enzymology glycogen liver level liver molecular weight muscle rat time Ammonium Compounds Animal Glucose Glucosyltransferases Glycogen Liver Liver Glycogen Molecular Weight Muscles Phosphates Rats Sodium Chloride Sulfates Time Factors Uracil Nucleotides High molecular weight glycogen has been prepared in vitro with muscle glycogen synthetase (uridine diphosphate glucose: α-1,4-glucan α-4-glucosyltransferase, EC 2.4.1.11) and liver or muscle branching enzyme (α-1,4-glucan: α-1,4-glucan 6-glycosyltransferase, EC 2.4.1.18) with uridine diphosphate glucose as glucose donor. The product obtained with a fresh synthetase preparation had a relatively low molecular weight like native muscle glycogen. The product synthesized with an aged synthetase preparation had a very high molecular weight similar to that of native liver glycogen. Ammonium sulfate and other salts inhibited the formation of high molecular weight glycogen by the aged synthetase preparation but not the total synhesis of the polysaccharide. The effect of the salts on the molecular weight of the product synthesized by the liver synthetase was much smaller. The changes in the molecular weight distribution of glycogen during synthesis with fresh, aged, and aged plus ammonium sulfate glycogen synthetases were studied. The results obtained are discussed. © 1970. Fil:Krisman, C.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Parodi, A.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1970 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v141_n1_p228_Krisman http://hdl.handle.net/20.500.12110/paper_00039861_v141_n1_p228_Krisman
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	ammonium derivative glucose glucosyltransferase glycogen phosphate pyrimidine nucleotide sodium chloride sulfate animal article biosynthesis enzymology glycogen liver level liver molecular weight muscle rat time Ammonium Compounds Animal Glucose Glucosyltransferases Glycogen Liver Liver Glycogen Molecular Weight Muscles Phosphates Rats Sodium Chloride Sulfates Time Factors Uracil Nucleotides
spellingShingle	ammonium derivative glucose glucosyltransferase glycogen phosphate pyrimidine nucleotide sodium chloride sulfate animal article biosynthesis enzymology glycogen liver level liver molecular weight muscle rat time Ammonium Compounds Animal Glucose Glucosyltransferases Glycogen Liver Liver Glycogen Molecular Weight Muscles Phosphates Rats Sodium Chloride Sulfates Time Factors Uracil Nucleotides Krisman de Fischman, Clara Rebeca Parodi, Armando José In vitro synthesis of particulate glycogen from uridine diphosphate glucose. III. Some properties of the product synthesized by muscle glycogen synthetase
topic_facet	ammonium derivative glucose glucosyltransferase glycogen phosphate pyrimidine nucleotide sodium chloride sulfate animal article biosynthesis enzymology glycogen liver level liver molecular weight muscle rat time Ammonium Compounds Animal Glucose Glucosyltransferases Glycogen Liver Liver Glycogen Molecular Weight Muscles Phosphates Rats Sodium Chloride Sulfates Time Factors Uracil Nucleotides
description	High molecular weight glycogen has been prepared in vitro with muscle glycogen synthetase (uridine diphosphate glucose: α-1,4-glucan α-4-glucosyltransferase, EC 2.4.1.11) and liver or muscle branching enzyme (α-1,4-glucan: α-1,4-glucan 6-glycosyltransferase, EC 2.4.1.18) with uridine diphosphate glucose as glucose donor. The product obtained with a fresh synthetase preparation had a relatively low molecular weight like native muscle glycogen. The product synthesized with an aged synthetase preparation had a very high molecular weight similar to that of native liver glycogen. Ammonium sulfate and other salts inhibited the formation of high molecular weight glycogen by the aged synthetase preparation but not the total synhesis of the polysaccharide. The effect of the salts on the molecular weight of the product synthesized by the liver synthetase was much smaller. The changes in the molecular weight distribution of glycogen during synthesis with fresh, aged, and aged plus ammonium sulfate glycogen synthetases were studied. The results obtained are discussed. © 1970.
author	Krisman de Fischman, Clara Rebeca Parodi, Armando José
author_facet	Krisman de Fischman, Clara Rebeca Parodi, Armando José
author_sort	Krisman de Fischman, Clara Rebeca
title	In vitro synthesis of particulate glycogen from uridine diphosphate glucose. III. Some properties of the product synthesized by muscle glycogen synthetase
title_short	In vitro synthesis of particulate glycogen from uridine diphosphate glucose. III. Some properties of the product synthesized by muscle glycogen synthetase
title_full	In vitro synthesis of particulate glycogen from uridine diphosphate glucose. III. Some properties of the product synthesized by muscle glycogen synthetase
title_fullStr	In vitro synthesis of particulate glycogen from uridine diphosphate glucose. III. Some properties of the product synthesized by muscle glycogen synthetase
title_full_unstemmed	In vitro synthesis of particulate glycogen from uridine diphosphate glucose. III. Some properties of the product synthesized by muscle glycogen synthetase
title_sort	in vitro synthesis of particulate glycogen from uridine diphosphate glucose. iii. some properties of the product synthesized by muscle glycogen synthetase
publishDate	1970
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v141_n1_p228_Krisman http://hdl.handle.net/20.500.12110/paper_00039861_v141_n1_p228_Krisman
work_keys_str_mv	AT krismandefischmanclararebeca invitrosynthesisofparticulateglycogenfromuridinediphosphateglucoseiiisomepropertiesoftheproductsynthesizedbymuscleglycogensynthetase AT parodiarmandojose invitrosynthesisofparticulateglycogenfromuridinediphosphateglucoseiiisomepropertiesoftheproductsynthesizedbymuscleglycogensynthetase
_version_	1768546517563473920

In vitro synthesis of particulate glycogen from uridine diphosphate glucose. III. Some properties of the product synthesized by muscle glycogen synthetase

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