Properties of synthetic and native liver glycogen

The properties of high molecular weight glycogen extracted from rat liver and of that prepared in vitro with muscle phosphorylase and liver branching enzyme have been compared. The stability at different pH values was measured spectrophotometrically for liver, corn, and synthetic glycogen. The forme...

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Autores principales: Parodi, Armando José, Krisman de Fischman, Clara Rebeca
Publicado: 1967
Materias:
pH
rat
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v121_n3_p769_Parodi
http://hdl.handle.net/20.500.12110/paper_00039861_v121_n3_p769_Parodi
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spelling paper:paper_00039861_v121_n3_p769_Parodi2023-06-08T14:24:53Z Properties of synthetic and native liver glycogen Parodi, Armando José Krisman de Fischman, Clara Rebeca glycogen metal animal article drug stability frogs and toads glycogen liver level heat molecular weight pH pigeon rat spectrophotometry ultrasound Animal Anura Drug Stability Glycogen Heat Hydrogen-Ion Concentration Liver Glycogen Metals Molecular Weight Pigeons Rats Spectrophotometry Ultrasonics The properties of high molecular weight glycogen extracted from rat liver and of that prepared in vitro with muscle phosphorylase and liver branching enzyme have been compared. The stability at different pH values was measured spectrophotometrically for liver, corn, and synthetic glycogen. The former is more labile, but the shape of the pH-stability curve is very similar for all of them. Borate, copper, and iron accelerate the decomposition of the three types of glycogen. Sonication produces breakdown but affects in the same way synthetic and liver glycogen. After shortening the outer chains with β-amylase, native liver glycogen becomes slightly more stable to acid treatment and decomposes giving smaller molecules than the untreated glycogen. Glycogen extracted from livers of toad and pigeon was similar in molecular weight distribution and acid lability to that of rat liver. Rat muscle glycogen had a molecular weight of about 8 million. © 1967. Fil:Parodi, A.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Krisman, C.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1967 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v121_n3_p769_Parodi http://hdl.handle.net/20.500.12110/paper_00039861_v121_n3_p769_Parodi
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic glycogen
metal
animal
article
drug stability
frogs and toads
glycogen liver level
heat
molecular weight
pH
pigeon
rat
spectrophotometry
ultrasound
Animal
Anura
Drug Stability
Glycogen
Heat
Hydrogen-Ion Concentration
Liver Glycogen
Metals
Molecular Weight
Pigeons
Rats
Spectrophotometry
Ultrasonics
spellingShingle glycogen
metal
animal
article
drug stability
frogs and toads
glycogen liver level
heat
molecular weight
pH
pigeon
rat
spectrophotometry
ultrasound
Animal
Anura
Drug Stability
Glycogen
Heat
Hydrogen-Ion Concentration
Liver Glycogen
Metals
Molecular Weight
Pigeons
Rats
Spectrophotometry
Ultrasonics
Parodi, Armando José
Krisman de Fischman, Clara Rebeca
Properties of synthetic and native liver glycogen
topic_facet glycogen
metal
animal
article
drug stability
frogs and toads
glycogen liver level
heat
molecular weight
pH
pigeon
rat
spectrophotometry
ultrasound
Animal
Anura
Drug Stability
Glycogen
Heat
Hydrogen-Ion Concentration
Liver Glycogen
Metals
Molecular Weight
Pigeons
Rats
Spectrophotometry
Ultrasonics
description The properties of high molecular weight glycogen extracted from rat liver and of that prepared in vitro with muscle phosphorylase and liver branching enzyme have been compared. The stability at different pH values was measured spectrophotometrically for liver, corn, and synthetic glycogen. The former is more labile, but the shape of the pH-stability curve is very similar for all of them. Borate, copper, and iron accelerate the decomposition of the three types of glycogen. Sonication produces breakdown but affects in the same way synthetic and liver glycogen. After shortening the outer chains with β-amylase, native liver glycogen becomes slightly more stable to acid treatment and decomposes giving smaller molecules than the untreated glycogen. Glycogen extracted from livers of toad and pigeon was similar in molecular weight distribution and acid lability to that of rat liver. Rat muscle glycogen had a molecular weight of about 8 million. © 1967.
author Parodi, Armando José
Krisman de Fischman, Clara Rebeca
author_facet Parodi, Armando José
Krisman de Fischman, Clara Rebeca
author_sort Parodi, Armando José
title Properties of synthetic and native liver glycogen
title_short Properties of synthetic and native liver glycogen
title_full Properties of synthetic and native liver glycogen
title_fullStr Properties of synthetic and native liver glycogen
title_full_unstemmed Properties of synthetic and native liver glycogen
title_sort properties of synthetic and native liver glycogen
publishDate 1967
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v121_n3_p769_Parodi
http://hdl.handle.net/20.500.12110/paper_00039861_v121_n3_p769_Parodi
work_keys_str_mv AT parodiarmandojose propertiesofsyntheticandnativeliverglycogen
AT krismandefischmanclararebeca propertiesofsyntheticandnativeliverglycogen
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