Multiphoton Excitation of Upconverting Nanoparticles in Pulsed Regime

Upconverting nanoparticles (UCNPs) present emission in the visible region upon irradiation with NIR light through a multiphoton mechanism. However, the long characteristic time of their emission has prevented the use of this kind of entities as multiphoton probes. We present a study on the use of er...

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Detalles Bibliográficos
Autores principales:	Hodak, Jose Hector, Etchenique, Roberto
Publicado:	2016
Materias:	Probes Characteristic time Emission behavior Excitation pulse Multi-photon microscopy Multiphoton excitation Orders of magnitude Under pulsed excitations Upconverting nanoparticles Nanoparticles
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032700_v88_n2_p1468_Hodak http://hdl.handle.net/20.500.12110/paper_00032700_v88_n2_p1468_Hodak
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00032700_v88_n2_p1468_Hodak
record_format	dspace
spelling	paper:paper_00032700_v88_n2_p1468_Hodak2023-06-08T14:24:15Z Multiphoton Excitation of Upconverting Nanoparticles in Pulsed Regime Hodak, Jose Hector Etchenique, Roberto Probes Characteristic time Emission behavior Excitation pulse Multi-photon microscopy Multiphoton excitation Orders of magnitude Under pulsed excitations Upconverting nanoparticles Nanoparticles Upconverting nanoparticles (UCNPs) present emission in the visible region upon irradiation with NIR light through a multiphoton mechanism. However, the long characteristic time of their emission has prevented the use of this kind of entities as multiphoton probes. We present a study on the use of erbium-containing UCNPs under pulsed excitation, showing that both the power density and the duration of the excitation pulse are key factors to understand the emission behavior. By adjusting power and excitation rate, we can obtain typical multiphoton z-axis focal exclusive excitation. These findings open the possibility of using UCNPs as probes for controlled localization of uncaging and imaging with multiphoton z-axis sectioning. We show that this can be achieved even at power densities several orders of magnitude lower than traditional multiphoton microscopies. © 2015 American Chemical Society. Fil:Hodak, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Etchenique, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032700_v88_n2_p1468_Hodak http://hdl.handle.net/20.500.12110/paper_00032700_v88_n2_p1468_Hodak
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Probes Characteristic time Emission behavior Excitation pulse Multi-photon microscopy Multiphoton excitation Orders of magnitude Under pulsed excitations Upconverting nanoparticles Nanoparticles
spellingShingle	Probes Characteristic time Emission behavior Excitation pulse Multi-photon microscopy Multiphoton excitation Orders of magnitude Under pulsed excitations Upconverting nanoparticles Nanoparticles Hodak, Jose Hector Etchenique, Roberto Multiphoton Excitation of Upconverting Nanoparticles in Pulsed Regime
topic_facet	Probes Characteristic time Emission behavior Excitation pulse Multi-photon microscopy Multiphoton excitation Orders of magnitude Under pulsed excitations Upconverting nanoparticles Nanoparticles
description	Upconverting nanoparticles (UCNPs) present emission in the visible region upon irradiation with NIR light through a multiphoton mechanism. However, the long characteristic time of their emission has prevented the use of this kind of entities as multiphoton probes. We present a study on the use of erbium-containing UCNPs under pulsed excitation, showing that both the power density and the duration of the excitation pulse are key factors to understand the emission behavior. By adjusting power and excitation rate, we can obtain typical multiphoton z-axis focal exclusive excitation. These findings open the possibility of using UCNPs as probes for controlled localization of uncaging and imaging with multiphoton z-axis sectioning. We show that this can be achieved even at power densities several orders of magnitude lower than traditional multiphoton microscopies. © 2015 American Chemical Society.
author	Hodak, Jose Hector Etchenique, Roberto
author_facet	Hodak, Jose Hector Etchenique, Roberto
author_sort	Hodak, Jose Hector
title	Multiphoton Excitation of Upconverting Nanoparticles in Pulsed Regime
title_short	Multiphoton Excitation of Upconverting Nanoparticles in Pulsed Regime
title_full	Multiphoton Excitation of Upconverting Nanoparticles in Pulsed Regime
title_fullStr	Multiphoton Excitation of Upconverting Nanoparticles in Pulsed Regime
title_full_unstemmed	Multiphoton Excitation of Upconverting Nanoparticles in Pulsed Regime
title_sort	multiphoton excitation of upconverting nanoparticles in pulsed regime
publishDate	2016
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032700_v88_n2_p1468_Hodak http://hdl.handle.net/20.500.12110/paper_00032700_v88_n2_p1468_Hodak
work_keys_str_mv	AT hodakjosehector multiphotonexcitationofupconvertingnanoparticlesinpulsedregime AT etcheniqueroberto multiphotonexcitationofupconvertingnanoparticlesinpulsedregime
_version_	1768544982749151232

Multiphoton Excitation of Upconverting Nanoparticles in Pulsed Regime

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