Chemical two-photon fluorescence
We describe a method based on a caged fluorescent molecule that can act as a chemical two-photon probe. It is composed of an organic fluorophore and a ruthenium-bipyridine complex that acts as a photoremovable quencher. For the fluorophore to be emissive, two independent photons must act on the mole...
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2015
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032700_v87_n8_p4363_Carrone http://hdl.handle.net/20.500.12110/paper_00032700_v87_n8_p4363_Carrone |
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paper:paper_00032700_v87_n8_p4363_Carrone |
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paper:paper_00032700_v87_n8_p4363_Carrone2023-06-08T14:24:14Z Chemical two-photon fluorescence Fluorescence Fluorophores High power lasers Molecules Probes Pulsed lasers Ruthenium compounds Ultrashort pulses Excitation intensity Fluorescent molecules Microscopy technique Organic fluorophores Ruthenium-bipyridine complexes Two photon fluorescence Two-photon excitations Ultra-short pulsed Photons We describe a method based on a caged fluorescent molecule that can act as a chemical two-photon probe. It is composed of an organic fluorophore and a ruthenium-bipyridine complex that acts as a photoremovable quencher. For the fluorophore to be emissive, two independent photons must act on the molecule: the first photon frees the fluorescent ligand from the Ru complex and the second photon excites the fluorescence. In this two-photon regime, the emission is not proportional to the excitation intensity but rather to its second power, as in traditional two-photon systems based on ultrashort pulsed high-power lasers. This quadratic relationship implies a much higher spatial precision on the z-axis when the probe is used in a microscopy technique. The chemical nature of the two-photon excitation mechanism allows the use of inexpensive low-power lasers. © 2015 American Chemical Society. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032700_v87_n8_p4363_Carrone http://hdl.handle.net/20.500.12110/paper_00032700_v87_n8_p4363_Carrone |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Fluorescence Fluorophores High power lasers Molecules Probes Pulsed lasers Ruthenium compounds Ultrashort pulses Excitation intensity Fluorescent molecules Microscopy technique Organic fluorophores Ruthenium-bipyridine complexes Two photon fluorescence Two-photon excitations Ultra-short pulsed Photons |
| spellingShingle |
Fluorescence Fluorophores High power lasers Molecules Probes Pulsed lasers Ruthenium compounds Ultrashort pulses Excitation intensity Fluorescent molecules Microscopy technique Organic fluorophores Ruthenium-bipyridine complexes Two photon fluorescence Two-photon excitations Ultra-short pulsed Photons Chemical two-photon fluorescence |
| topic_facet |
Fluorescence Fluorophores High power lasers Molecules Probes Pulsed lasers Ruthenium compounds Ultrashort pulses Excitation intensity Fluorescent molecules Microscopy technique Organic fluorophores Ruthenium-bipyridine complexes Two photon fluorescence Two-photon excitations Ultra-short pulsed Photons |
| description |
We describe a method based on a caged fluorescent molecule that can act as a chemical two-photon probe. It is composed of an organic fluorophore and a ruthenium-bipyridine complex that acts as a photoremovable quencher. For the fluorophore to be emissive, two independent photons must act on the molecule: the first photon frees the fluorescent ligand from the Ru complex and the second photon excites the fluorescence. In this two-photon regime, the emission is not proportional to the excitation intensity but rather to its second power, as in traditional two-photon systems based on ultrashort pulsed high-power lasers. This quadratic relationship implies a much higher spatial precision on the z-axis when the probe is used in a microscopy technique. The chemical nature of the two-photon excitation mechanism allows the use of inexpensive low-power lasers. © 2015 American Chemical Society. |
| title |
Chemical two-photon fluorescence |
| title_short |
Chemical two-photon fluorescence |
| title_full |
Chemical two-photon fluorescence |
| title_fullStr |
Chemical two-photon fluorescence |
| title_full_unstemmed |
Chemical two-photon fluorescence |
| title_sort |
chemical two-photon fluorescence |
| publishDate |
2015 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032700_v87_n8_p4363_Carrone http://hdl.handle.net/20.500.12110/paper_00032700_v87_n8_p4363_Carrone |
| _version_ |
1768545167870001152 |