Fast optical pH manipulation and imaging

We describe a complete system for optical pH manipulation and imaging. The system consists of a photoactive Ruthenium complex capable of inducing a change of more than 5 pH units at the nanosecond time scale. A compatible imaging system acquires microscopic pH images at 1200 fps using a nonexpensive...

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Publicado: 2012
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Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032700_v84_n13_p5618_Filevich
http://hdl.handle.net/20.500.12110/paper_00032700_v84_n13_p5618_Filevich
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Sumario:We describe a complete system for optical pH manipulation and imaging. The system consists of a photoactive Ruthenium complex capable of inducing a change of more than 5 pH units at the nanosecond time scale. A compatible imaging system acquires microscopic pH images at 1200 fps using a nonexpensive commercial digital camera and an LED illumination system. We use the system as a superb tool to investigate flow in Flow Injection Analysis (FIA) models. © 2012 American Chemical Society.