Use of an antimicrobial protein for endotoxin detection in a competitive electrochemical assay

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Endotoxins, also referred to as pyrogens, are part of the cellular walls of Gram-negative bacteria and are capable of inducing fever when entering into the blood stream. Current methods of detection involve the use of either amoebocytes from horseshoe crab that clots upon exposure to endotoxin or a...

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Autores principales:	Priano, Graciela Inés, Battaglini, Fernando
Publicado:	2005
Materias:	Endotoxins Gram-negative bacteria Horseshoe crab Pyrogens Assays Bacteria Blood Cells Electrodes Electrostatics Polymers Toxic materials Proteins carboxymethyldextran endotoxin horseradish peroxidase lipopolysaccharide polymer protein pyrogen water amperometry article assay covalent bond electricity electrochemistry electrode ellipsometry Adsorption Anti-Infective Agents Catalysis Electrochemistry Horseradish Peroxidase Lipopolysaccharides Molecular Structure Oxidation-Reduction
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032700_v77_n15_p4976_Priano http://hdl.handle.net/20.500.12110/paper_00032700_v77_n15_p4976_Priano
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00032700_v77_n15_p4976_Priano
record_format	dspace
spelling	paper:paper_00032700_v77_n15_p4976_Priano2023-06-08T14:24:11Z Use of an antimicrobial protein for endotoxin detection in a competitive electrochemical assay Priano, Graciela Inés Battaglini, Fernando Endotoxins Gram-negative bacteria Horseshoe crab Pyrogens Assays Bacteria Blood Cells Electrodes Electrostatics Polymers Toxic materials Proteins carboxymethyldextran endotoxin horseradish peroxidase lipopolysaccharide polymer protein pyrogen water amperometry article assay covalent bond electricity electrochemistry electrode ellipsometry Adsorption Anti-Infective Agents Catalysis Electrochemistry Electrodes Endotoxins Horseradish Peroxidase Lipopolysaccharides Molecular Structure Oxidation-Reduction Endotoxins, also referred to as pyrogens, are part of the cellular walls of Gram-negative bacteria and are capable of inducing fever when entering into the blood stream. Current methods of detection involve the use of either amoebocytes from horseshoe crab that clots upon exposure to endotoxin or a live assay on rabbits. In this work, the detection of endotoxins by an electrochemical competitive assay is presented. Two configurations of modified electrodes were constructed using a recombinant endotoxin neutralizing protein (ENP) as recognition element. A modified lipopolysaccharide with horseradish peroxidase was used for the competitive assay. Modified electrodes constructed by electrostatic interaction of ENP and an electroactive polymer can detect the presence of endotoxins in concentrations as low as 0.2EU mL-1, below the limit imposed for water in injectable drugs in the American Pharmacopoeia. Modified electrodes constructed by covalent linking of ENP to a carboxymethyl dextran matrix bound to the electrode show a better dynamic range but a higher detection limit. © 2005 American Chemical Society. Fil:Priano, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Battaglini, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2005 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032700_v77_n15_p4976_Priano http://hdl.handle.net/20.500.12110/paper_00032700_v77_n15_p4976_Priano
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Endotoxins Gram-negative bacteria Horseshoe crab Pyrogens Assays Bacteria Blood Cells Electrodes Electrostatics Polymers Toxic materials Proteins carboxymethyldextran endotoxin horseradish peroxidase lipopolysaccharide polymer protein pyrogen water amperometry article assay covalent bond electricity electrochemistry electrode ellipsometry Adsorption Anti-Infective Agents Catalysis Electrochemistry Electrodes Endotoxins Horseradish Peroxidase Lipopolysaccharides Molecular Structure Oxidation-Reduction
spellingShingle	Endotoxins Gram-negative bacteria Horseshoe crab Pyrogens Assays Bacteria Blood Cells Electrodes Electrostatics Polymers Toxic materials Proteins carboxymethyldextran endotoxin horseradish peroxidase lipopolysaccharide polymer protein pyrogen water amperometry article assay covalent bond electricity electrochemistry electrode ellipsometry Adsorption Anti-Infective Agents Catalysis Electrochemistry Electrodes Endotoxins Horseradish Peroxidase Lipopolysaccharides Molecular Structure Oxidation-Reduction Priano, Graciela Inés Battaglini, Fernando Use of an antimicrobial protein for endotoxin detection in a competitive electrochemical assay
topic_facet	Endotoxins Gram-negative bacteria Horseshoe crab Pyrogens Assays Bacteria Blood Cells Electrodes Electrostatics Polymers Toxic materials Proteins carboxymethyldextran endotoxin horseradish peroxidase lipopolysaccharide polymer protein pyrogen water amperometry article assay covalent bond electricity electrochemistry electrode ellipsometry Adsorption Anti-Infective Agents Catalysis Electrochemistry Electrodes Endotoxins Horseradish Peroxidase Lipopolysaccharides Molecular Structure Oxidation-Reduction
description	Endotoxins, also referred to as pyrogens, are part of the cellular walls of Gram-negative bacteria and are capable of inducing fever when entering into the blood stream. Current methods of detection involve the use of either amoebocytes from horseshoe crab that clots upon exposure to endotoxin or a live assay on rabbits. In this work, the detection of endotoxins by an electrochemical competitive assay is presented. Two configurations of modified electrodes were constructed using a recombinant endotoxin neutralizing protein (ENP) as recognition element. A modified lipopolysaccharide with horseradish peroxidase was used for the competitive assay. Modified electrodes constructed by electrostatic interaction of ENP and an electroactive polymer can detect the presence of endotoxins in concentrations as low as 0.2EU mL-1, below the limit imposed for water in injectable drugs in the American Pharmacopoeia. Modified electrodes constructed by covalent linking of ENP to a carboxymethyl dextran matrix bound to the electrode show a better dynamic range but a higher detection limit. © 2005 American Chemical Society.
author	Priano, Graciela Inés Battaglini, Fernando
author_facet	Priano, Graciela Inés Battaglini, Fernando
author_sort	Priano, Graciela Inés
title	Use of an antimicrobial protein for endotoxin detection in a competitive electrochemical assay
title_short	Use of an antimicrobial protein for endotoxin detection in a competitive electrochemical assay
title_full	Use of an antimicrobial protein for endotoxin detection in a competitive electrochemical assay
title_fullStr	Use of an antimicrobial protein for endotoxin detection in a competitive electrochemical assay
title_full_unstemmed	Use of an antimicrobial protein for endotoxin detection in a competitive electrochemical assay
title_sort	use of an antimicrobial protein for endotoxin detection in a competitive electrochemical assay
publishDate	2005
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032700_v77_n15_p4976_Priano http://hdl.handle.net/20.500.12110/paper_00032700_v77_n15_p4976_Priano
work_keys_str_mv	AT prianogracielaines useofanantimicrobialproteinforendotoxindetectioninacompetitiveelectrochemicalassay AT battaglinifernando useofanantimicrobialproteinforendotoxindetectioninacompetitiveelectrochemicalassay
_version_	1768543350632218624

Use of an antimicrobial protein for endotoxin detection in a competitive electrochemical assay

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