Layer-by-layer self-assembly of glucose oxidase and os(Bpy)2CIPyCH2NH-poly(allylamine) bioelectrode

The uptake of glucose oxidase (GOx) onto a polycationic redox polymer (PAA-Os)-modified surface, by adsorption from dilute aqueous GOx solutions, was followed by the quartz crystal microbalance (QCM) and shows double exponential kinetics. The electrochemistry of the layer-by-layer-deposited redox-ac...

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Autores principales:	Calvo, Ernesto Julio, Etchenique, Roberto
Publicado:	2001
Materias:	Redox polymers Adsorption Agglomeration Cyclic voltammetry Electrochemistry Electrodes Glucose Gold Polymers Quartz Self assembly Enzymes 2,2' diaminoethyldisulfide alkane derivative beta glucose enzyme glucose glucose derivative glucose oxidase gold osmium derivative polyamine derivative polycation polymer silicon dioxide unclassified drug water adsorption aqueous solution article atomic force microscopy cyclic potentiometry electrochemistry electrode kinetics oxidation reduction reaction protein assembly Biosensing Techniques Glucose Oxidase Organometallic Compounds Oxidation-Reduction
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032700_v73_n6_p1161_Calvo http://hdl.handle.net/20.500.12110/paper_00032700_v73_n6_p1161_Calvo
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00032700_v73_n6_p1161_Calvo
record_format	dspace
spelling	paper:paper_00032700_v73_n6_p1161_Calvo2023-06-08T14:24:10Z Layer-by-layer self-assembly of glucose oxidase and os(Bpy)2CIPyCH2NH-poly(allylamine) bioelectrode Calvo, Ernesto Julio Etchenique, Roberto Redox polymers Adsorption Agglomeration Cyclic voltammetry Electrochemistry Electrodes Glucose Gold Polymers Quartz Self assembly Enzymes 2,2' diaminoethyldisulfide alkane derivative beta glucose enzyme glucose glucose derivative glucose oxidase gold osmium derivative polyamine derivative polycation polymer silicon dioxide unclassified drug water adsorption aqueous solution article atomic force microscopy cyclic potentiometry electrochemistry electrode kinetics oxidation reduction reaction protein assembly Biosensing Techniques Electrochemistry Glucose Oxidase Organometallic Compounds Oxidation-Reduction The uptake of glucose oxidase (GOx) onto a polycationic redox polymer (PAA-Os)-modified surface, by adsorption from dilute aqueous GOx solutions, was followed by the quartz crystal microbalance (QCM) and shows double exponential kinetics. The electrochemistry of the layer-by-layer-deposited redox-active polymer was followed by cyclic voltammetry in glucose-free solutions, and the enzyme catalysis mediated by the redox polymer was studied in β-D-glucose-containing solutions. AFM studies of the different layers showed the existence of large two dimension enzyme aggregates on the osmium polymer for 1 μM GOx and less aggregation for 50 nM GOx solutions. When the short alkanethiol, 2,2′-diaminoethyldisulfide was preadsorbed onto gold, a monoexponential adsorption law was observed, and single GOx enzyme molecules could be seen on the surface where the enzyme was adsorbed from 50 nM GOx in water. Fil:Calvo, E.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Etchenique, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2001 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032700_v73_n6_p1161_Calvo http://hdl.handle.net/20.500.12110/paper_00032700_v73_n6_p1161_Calvo
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Redox polymers Adsorption Agglomeration Cyclic voltammetry Electrochemistry Electrodes Glucose Gold Polymers Quartz Self assembly Enzymes 2,2' diaminoethyldisulfide alkane derivative beta glucose enzyme glucose glucose derivative glucose oxidase gold osmium derivative polyamine derivative polycation polymer silicon dioxide unclassified drug water adsorption aqueous solution article atomic force microscopy cyclic potentiometry electrochemistry electrode kinetics oxidation reduction reaction protein assembly Biosensing Techniques Electrochemistry Glucose Oxidase Organometallic Compounds Oxidation-Reduction
spellingShingle	Redox polymers Adsorption Agglomeration Cyclic voltammetry Electrochemistry Electrodes Glucose Gold Polymers Quartz Self assembly Enzymes 2,2' diaminoethyldisulfide alkane derivative beta glucose enzyme glucose glucose derivative glucose oxidase gold osmium derivative polyamine derivative polycation polymer silicon dioxide unclassified drug water adsorption aqueous solution article atomic force microscopy cyclic potentiometry electrochemistry electrode kinetics oxidation reduction reaction protein assembly Biosensing Techniques Electrochemistry Glucose Oxidase Organometallic Compounds Oxidation-Reduction Calvo, Ernesto Julio Etchenique, Roberto Layer-by-layer self-assembly of glucose oxidase and os(Bpy)2CIPyCH2NH-poly(allylamine) bioelectrode
topic_facet	Redox polymers Adsorption Agglomeration Cyclic voltammetry Electrochemistry Electrodes Glucose Gold Polymers Quartz Self assembly Enzymes 2,2' diaminoethyldisulfide alkane derivative beta glucose enzyme glucose glucose derivative glucose oxidase gold osmium derivative polyamine derivative polycation polymer silicon dioxide unclassified drug water adsorption aqueous solution article atomic force microscopy cyclic potentiometry electrochemistry electrode kinetics oxidation reduction reaction protein assembly Biosensing Techniques Electrochemistry Glucose Oxidase Organometallic Compounds Oxidation-Reduction
description	The uptake of glucose oxidase (GOx) onto a polycationic redox polymer (PAA-Os)-modified surface, by adsorption from dilute aqueous GOx solutions, was followed by the quartz crystal microbalance (QCM) and shows double exponential kinetics. The electrochemistry of the layer-by-layer-deposited redox-active polymer was followed by cyclic voltammetry in glucose-free solutions, and the enzyme catalysis mediated by the redox polymer was studied in β-D-glucose-containing solutions. AFM studies of the different layers showed the existence of large two dimension enzyme aggregates on the osmium polymer for 1 μM GOx and less aggregation for 50 nM GOx solutions. When the short alkanethiol, 2,2′-diaminoethyldisulfide was preadsorbed onto gold, a monoexponential adsorption law was observed, and single GOx enzyme molecules could be seen on the surface where the enzyme was adsorbed from 50 nM GOx in water.
author	Calvo, Ernesto Julio Etchenique, Roberto
author_facet	Calvo, Ernesto Julio Etchenique, Roberto
author_sort	Calvo, Ernesto Julio
title	Layer-by-layer self-assembly of glucose oxidase and os(Bpy)2CIPyCH2NH-poly(allylamine) bioelectrode
title_short	Layer-by-layer self-assembly of glucose oxidase and os(Bpy)2CIPyCH2NH-poly(allylamine) bioelectrode
title_full	Layer-by-layer self-assembly of glucose oxidase and os(Bpy)2CIPyCH2NH-poly(allylamine) bioelectrode
title_fullStr	Layer-by-layer self-assembly of glucose oxidase and os(Bpy)2CIPyCH2NH-poly(allylamine) bioelectrode
title_full_unstemmed	Layer-by-layer self-assembly of glucose oxidase and os(Bpy)2CIPyCH2NH-poly(allylamine) bioelectrode
title_sort	layer-by-layer self-assembly of glucose oxidase and os(bpy)2cipych2nh-poly(allylamine) bioelectrode
publishDate	2001
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032700_v73_n6_p1161_Calvo http://hdl.handle.net/20.500.12110/paper_00032700_v73_n6_p1161_Calvo
work_keys_str_mv	AT calvoernestojulio layerbylayerselfassemblyofglucoseoxidaseandosbpy2cipych2nhpolyallylaminebioelectrode AT etcheniqueroberto layerbylayerselfassemblyofglucoseoxidaseandosbpy2cipych2nhpolyallylaminebioelectrode
_version_	1768541871526641664

Layer-by-layer self-assembly of glucose oxidase and os(Bpy)2CIPyCH2NH-poly(allylamine) bioelectrode

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