Assay of glucoprotein formation from uridine diphosphate glucose by a rapid filter-paper technique
A rapid, simple, sensitive and reproducible filter-paper assay was developed to follow the glucose transfer from uridine diphosphate [14C]glucose to protein or protein-bound oligosaccharides. This method could be useful for following the formation of any glycoprotein. Rectangles of trichloroacetic a...
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1975
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032697_v66_n2_p584_Krisman http://hdl.handle.net/20.500.12110/paper_00032697_v66_n2_p584_Krisman |
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paper:paper_00032697_v66_n2_p584_Krisman2023-06-08T14:24:06Z Assay of glucoprotein formation from uridine diphosphate glucose by a rapid filter-paper technique Krisman de Fischman, Clara Rebeca Barengo, Renee carbon glycoprotein uridine diphosphate glucose uridine diphosphate sugar animal article biosynthesis kinetics liver metabolism paper rat technique time Animal Carbon Radioisotopes Evaluation Studies Glycoproteins Kinetics Liver Methods Paper Rats Time Factors Uridine Diphosphate Glucose Uridine Diphosphate Sugars A rapid, simple, sensitive and reproducible filter-paper assay was developed to follow the glucose transfer from uridine diphosphate [14C]glucose to protein or protein-bound oligosaccharides. This method could be useful for following the formation of any glycoprotein. Rectangles of trichloroacetic acid-treated filter paper are introduced into a test tube containing the reaction mixture. The papers are submitted to a batch washing-procedure that ensures the removal of the compounds that are not trichloroacetic acid-precipitable. After scintillation counting, the paper can be reused to study the acid lability of the glucoprotein. © 1975. Fil:Krisman, C.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Barengo, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1975 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032697_v66_n2_p584_Krisman http://hdl.handle.net/20.500.12110/paper_00032697_v66_n2_p584_Krisman |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
carbon glycoprotein uridine diphosphate glucose uridine diphosphate sugar animal article biosynthesis kinetics liver metabolism paper rat technique time Animal Carbon Radioisotopes Evaluation Studies Glycoproteins Kinetics Liver Methods Paper Rats Time Factors Uridine Diphosphate Glucose Uridine Diphosphate Sugars |
| spellingShingle |
carbon glycoprotein uridine diphosphate glucose uridine diphosphate sugar animal article biosynthesis kinetics liver metabolism paper rat technique time Animal Carbon Radioisotopes Evaluation Studies Glycoproteins Kinetics Liver Methods Paper Rats Time Factors Uridine Diphosphate Glucose Uridine Diphosphate Sugars Krisman de Fischman, Clara Rebeca Barengo, Renee Assay of glucoprotein formation from uridine diphosphate glucose by a rapid filter-paper technique |
| topic_facet |
carbon glycoprotein uridine diphosphate glucose uridine diphosphate sugar animal article biosynthesis kinetics liver metabolism paper rat technique time Animal Carbon Radioisotopes Evaluation Studies Glycoproteins Kinetics Liver Methods Paper Rats Time Factors Uridine Diphosphate Glucose Uridine Diphosphate Sugars |
| description |
A rapid, simple, sensitive and reproducible filter-paper assay was developed to follow the glucose transfer from uridine diphosphate [14C]glucose to protein or protein-bound oligosaccharides. This method could be useful for following the formation of any glycoprotein. Rectangles of trichloroacetic acid-treated filter paper are introduced into a test tube containing the reaction mixture. The papers are submitted to a batch washing-procedure that ensures the removal of the compounds that are not trichloroacetic acid-precipitable. After scintillation counting, the paper can be reused to study the acid lability of the glucoprotein. © 1975. |
| author |
Krisman de Fischman, Clara Rebeca Barengo, Renee |
| author_facet |
Krisman de Fischman, Clara Rebeca Barengo, Renee |
| author_sort |
Krisman de Fischman, Clara Rebeca |
| title |
Assay of glucoprotein formation from uridine diphosphate glucose by a rapid filter-paper technique |
| title_short |
Assay of glucoprotein formation from uridine diphosphate glucose by a rapid filter-paper technique |
| title_full |
Assay of glucoprotein formation from uridine diphosphate glucose by a rapid filter-paper technique |
| title_fullStr |
Assay of glucoprotein formation from uridine diphosphate glucose by a rapid filter-paper technique |
| title_full_unstemmed |
Assay of glucoprotein formation from uridine diphosphate glucose by a rapid filter-paper technique |
| title_sort |
assay of glucoprotein formation from uridine diphosphate glucose by a rapid filter-paper technique |
| publishDate |
1975 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032697_v66_n2_p584_Krisman http://hdl.handle.net/20.500.12110/paper_00032697_v66_n2_p584_Krisman |
| work_keys_str_mv |
AT krismandefischmanclararebeca assayofglucoproteinformationfromuridinediphosphateglucosebyarapidfilterpapertechnique AT barengorenee assayofglucoproteinformationfromuridinediphosphateglucosebyarapidfilterpapertechnique |
| _version_ |
1768542341482676224 |