Milk lactate determination with a rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped- flow system
The high sensitivity that can be attained using a bienzymatic system and mediated by the redox polymer [Os(bpy)2 ClPyCH2NHpoly(allylamine)] (Os-PAA), has been verified by on-line interfacing of a rotating bioreactor and continuous-flow/stopped-flow/continuous-flow processing. When the hydrogen perox...
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2003
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032670_v498_n1-2_p155_Torriero http://hdl.handle.net/20.500.12110/paper_00032670_v498_n1-2_p155_Torriero |
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paper:paper_00032670_v498_n1-2_p155_Torriero2023-06-08T14:23:57Z Milk lactate determination with a rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped- flow system Battaglini, Fernando Bioreactor Biosensors Flow system Horseradish peroxidase Lactate Lactate oxidase Milk Osmium Carbon Electrodes Enzymes Flow of fluids Hydrogen peroxide Organic compounds Osmium Polymers Milk lactate determination Bioreactors horseradish peroxidase hydrogen peroxide lactate 2 monooxygenase lactic acid osmium derivative polymer article bioreactor continuous flow reactor electric current electrode electron transport enzyme immobilization milk oxidation reduction reaction priority journal reproducibility rotation Armoracia rusticana The high sensitivity that can be attained using a bienzymatic system and mediated by the redox polymer [Os(bpy)2 ClPyCH2NHpoly(allylamine)] (Os-PAA), has been verified by on-line interfacing of a rotating bioreactor and continuous-flow/stopped-flow/continuous-flow processing. When the hydrogen peroxide formed by LOx layer reaches the inner layer, the electronic flow between the immobilized peroxidase and the electrode surface produces a current, proportional to lactate concentration. The determination of lactate was possible with a limit of detection of 5nmoll -1 in the processing of as many as 30 samples per hour. This arrangement allows working in undiluted milk samples with a good stability and reproducibility. Horseradish peroxidase [EC 1.11.1.7] and Os-PAA were covalently immobilized on the glassy carbon electrode surface (upper cell body), lactate oxidase [EC 1.1.3.x] was immobilized on a disk that can be rotated. © 2003 Elsevier B.V. All rights reserved. Fil:Battaglini, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2003 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032670_v498_n1-2_p155_Torriero http://hdl.handle.net/20.500.12110/paper_00032670_v498_n1-2_p155_Torriero |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Bioreactor Biosensors Flow system Horseradish peroxidase Lactate Lactate oxidase Milk Osmium Carbon Electrodes Enzymes Flow of fluids Hydrogen peroxide Organic compounds Osmium Polymers Milk lactate determination Bioreactors horseradish peroxidase hydrogen peroxide lactate 2 monooxygenase lactic acid osmium derivative polymer article bioreactor continuous flow reactor electric current electrode electron transport enzyme immobilization milk oxidation reduction reaction priority journal reproducibility rotation Armoracia rusticana |
| spellingShingle |
Bioreactor Biosensors Flow system Horseradish peroxidase Lactate Lactate oxidase Milk Osmium Carbon Electrodes Enzymes Flow of fluids Hydrogen peroxide Organic compounds Osmium Polymers Milk lactate determination Bioreactors horseradish peroxidase hydrogen peroxide lactate 2 monooxygenase lactic acid osmium derivative polymer article bioreactor continuous flow reactor electric current electrode electron transport enzyme immobilization milk oxidation reduction reaction priority journal reproducibility rotation Armoracia rusticana Battaglini, Fernando Milk lactate determination with a rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped- flow system |
| topic_facet |
Bioreactor Biosensors Flow system Horseradish peroxidase Lactate Lactate oxidase Milk Osmium Carbon Electrodes Enzymes Flow of fluids Hydrogen peroxide Organic compounds Osmium Polymers Milk lactate determination Bioreactors horseradish peroxidase hydrogen peroxide lactate 2 monooxygenase lactic acid osmium derivative polymer article bioreactor continuous flow reactor electric current electrode electron transport enzyme immobilization milk oxidation reduction reaction priority journal reproducibility rotation Armoracia rusticana |
| description |
The high sensitivity that can be attained using a bienzymatic system and mediated by the redox polymer [Os(bpy)2 ClPyCH2NHpoly(allylamine)] (Os-PAA), has been verified by on-line interfacing of a rotating bioreactor and continuous-flow/stopped-flow/continuous-flow processing. When the hydrogen peroxide formed by LOx layer reaches the inner layer, the electronic flow between the immobilized peroxidase and the electrode surface produces a current, proportional to lactate concentration. The determination of lactate was possible with a limit of detection of 5nmoll -1 in the processing of as many as 30 samples per hour. This arrangement allows working in undiluted milk samples with a good stability and reproducibility. Horseradish peroxidase [EC 1.11.1.7] and Os-PAA were covalently immobilized on the glassy carbon electrode surface (upper cell body), lactate oxidase [EC 1.1.3.x] was immobilized on a disk that can be rotated. © 2003 Elsevier B.V. All rights reserved. |
| author |
Battaglini, Fernando |
| author_facet |
Battaglini, Fernando |
| author_sort |
Battaglini, Fernando |
| title |
Milk lactate determination with a rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped- flow system |
| title_short |
Milk lactate determination with a rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped- flow system |
| title_full |
Milk lactate determination with a rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped- flow system |
| title_fullStr |
Milk lactate determination with a rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped- flow system |
| title_full_unstemmed |
Milk lactate determination with a rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped- flow system |
| title_sort |
milk lactate determination with a rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped- flow system |
| publishDate |
2003 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032670_v498_n1-2_p155_Torriero http://hdl.handle.net/20.500.12110/paper_00032670_v498_n1-2_p155_Torriero |
| work_keys_str_mv |
AT battaglinifernando milklactatedeterminationwitharotatingbioreactorbasedonanelectrontransfermediatedbyosmiumcomplexesincorporatingacontinuousflowstoppedflowsystem |
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