Multiway analysis through direct excitation-emission matrix imaging
In this work, a direct in-flow methodology for the acquisition of excitation-emission fluorescence matrices is presented. The system is particularly suited for measurements in the order of tens of milliseconds. A light source operated in continuous mode is dispersed through a grating and focused ont...
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2018
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032670_v1032_n_p32_Alcaraz http://hdl.handle.net/20.500.12110/paper_00032670_v1032_n_p32_Alcaraz |
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paper:paper_00032670_v1032_n_p32_Alcaraz2023-06-08T14:23:54Z Multiway analysis through direct excitation-emission matrix imaging Chemometrics Excitation-emission matrix Higher-order data analysis Imaging Charge coupled devices Chromatographic analysis Data acquisition Imaging techniques Light sources Mixtures Chemometrics Excitation emission fluorescence Excitation emission matrices Fast data acquisition Higher-order Interference filters Spectral calibration Theoretical performance Matrix algebra accuracy algorithm Article calculation calibration excitation emission matrix imaging image analysis imaging light liquid chromatography luminance mathematical analysis measurement Parallel factor analysis priority journal reliability validity In this work, a direct in-flow methodology for the acquisition of excitation-emission fluorescence matrices is presented. The system is particularly suited for measurements in the order of tens of milliseconds. A light source operated in continuous mode is dispersed through a grating and focused onto a square-section capillary. Under the spatially resolved excitation, the emission is collected, dispersed through a second grating and further focused onto a CCD array sensor. To allow the wavelength accuracy, a spectral calibration was performed registering the scattering signal of a dispersive element using interference filters ranging from 340 nm to 740 nm. The theoretical performance of the method was analyzed and second-order data obtained for different analyte mixtures are presented and discussed. PARAFAC was applied to evaluate the trilinearity of the obtained data. Mathematical evaluation by means of the criterion of similarity corroborates the agreement between experimental pure spectra and spectral profiles retrieved from PARAFAC. Moreover, the feasibility of the spectrometer to obtain second-order data for analyses with quantitative aims was demonstrated. Finally, fast data acquisition was proved by monitoring a chromatographic analysis of dye mixtures for the generation of third-order LC-EEM data. Here, an improvement in the resolution of the different instrumental modes was demonstrated. © 2018 Elsevier B.V. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032670_v1032_n_p32_Alcaraz http://hdl.handle.net/20.500.12110/paper_00032670_v1032_n_p32_Alcaraz |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Chemometrics Excitation-emission matrix Higher-order data analysis Imaging Charge coupled devices Chromatographic analysis Data acquisition Imaging techniques Light sources Mixtures Chemometrics Excitation emission fluorescence Excitation emission matrices Fast data acquisition Higher-order Interference filters Spectral calibration Theoretical performance Matrix algebra accuracy algorithm Article calculation calibration excitation emission matrix imaging image analysis imaging light liquid chromatography luminance mathematical analysis measurement Parallel factor analysis priority journal reliability validity |
| spellingShingle |
Chemometrics Excitation-emission matrix Higher-order data analysis Imaging Charge coupled devices Chromatographic analysis Data acquisition Imaging techniques Light sources Mixtures Chemometrics Excitation emission fluorescence Excitation emission matrices Fast data acquisition Higher-order Interference filters Spectral calibration Theoretical performance Matrix algebra accuracy algorithm Article calculation calibration excitation emission matrix imaging image analysis imaging light liquid chromatography luminance mathematical analysis measurement Parallel factor analysis priority journal reliability validity Multiway analysis through direct excitation-emission matrix imaging |
| topic_facet |
Chemometrics Excitation-emission matrix Higher-order data analysis Imaging Charge coupled devices Chromatographic analysis Data acquisition Imaging techniques Light sources Mixtures Chemometrics Excitation emission fluorescence Excitation emission matrices Fast data acquisition Higher-order Interference filters Spectral calibration Theoretical performance Matrix algebra accuracy algorithm Article calculation calibration excitation emission matrix imaging image analysis imaging light liquid chromatography luminance mathematical analysis measurement Parallel factor analysis priority journal reliability validity |
| description |
In this work, a direct in-flow methodology for the acquisition of excitation-emission fluorescence matrices is presented. The system is particularly suited for measurements in the order of tens of milliseconds. A light source operated in continuous mode is dispersed through a grating and focused onto a square-section capillary. Under the spatially resolved excitation, the emission is collected, dispersed through a second grating and further focused onto a CCD array sensor. To allow the wavelength accuracy, a spectral calibration was performed registering the scattering signal of a dispersive element using interference filters ranging from 340 nm to 740 nm. The theoretical performance of the method was analyzed and second-order data obtained for different analyte mixtures are presented and discussed. PARAFAC was applied to evaluate the trilinearity of the obtained data. Mathematical evaluation by means of the criterion of similarity corroborates the agreement between experimental pure spectra and spectral profiles retrieved from PARAFAC. Moreover, the feasibility of the spectrometer to obtain second-order data for analyses with quantitative aims was demonstrated. Finally, fast data acquisition was proved by monitoring a chromatographic analysis of dye mixtures for the generation of third-order LC-EEM data. Here, an improvement in the resolution of the different instrumental modes was demonstrated. © 2018 Elsevier B.V. |
| title |
Multiway analysis through direct excitation-emission matrix imaging |
| title_short |
Multiway analysis through direct excitation-emission matrix imaging |
| title_full |
Multiway analysis through direct excitation-emission matrix imaging |
| title_fullStr |
Multiway analysis through direct excitation-emission matrix imaging |
| title_full_unstemmed |
Multiway analysis through direct excitation-emission matrix imaging |
| title_sort |
multiway analysis through direct excitation-emission matrix imaging |
| publishDate |
2018 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032670_v1032_n_p32_Alcaraz http://hdl.handle.net/20.500.12110/paper_00032670_v1032_n_p32_Alcaraz |
| _version_ |
1768544755628638208 |