Effect of different diets on digestive enzyme activities, in vitro digestibility, and midgut gland structure in juvenile crayfish, Cherax quadricarinatus

This study investigated the effects of food quality on digestive enzyme activities, in vitro protein digestibility and histological traits of the midgut gland in juvenile crayfish Cherax quadricarinatus. Animals of a wide weight range were fed different diets: two commercial diets with high or low l...

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Autores principales:	Sacristán, Hernán Javier, Fernández Gimenez, Analia Verónica
Publicado:	2016
Materias:	Cherax quadricarinatus digestive enzymes feed formulations in vitro protein digestibility midgut gland artificial diet crayfish digestibility enzyme activity food quality histology juvenile lipid malnutrition protein Animalia Astacoidea
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00017272_v97_n4_p407_Sacristan http://hdl.handle.net/20.500.12110/paper_00017272_v97_n4_p407_Sacristan
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00017272_v97_n4_p407_Sacristan
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spelling	paper:paper_00017272_v97_n4_p407_Sacristan2023-06-08T14:21:26Z Effect of different diets on digestive enzyme activities, in vitro digestibility, and midgut gland structure in juvenile crayfish, Cherax quadricarinatus Sacristán, Hernán Javier Fernández Gimenez, Analia Verónica Cherax quadricarinatus digestive enzymes feed formulations in vitro protein digestibility midgut gland artificial diet crayfish digestibility enzyme activity food quality histology juvenile lipid malnutrition protein Animalia Astacoidea Cherax quadricarinatus This study investigated the effects of food quality on digestive enzyme activities, in vitro protein digestibility and histological traits of the midgut gland in juvenile crayfish Cherax quadricarinatus. Animals of a wide weight range were fed different diets: two commercial diets with high or low lipid content (high lipid and low lipid, respectively) and were compared with a reference diet (RF) previously formulated for this species. Proteinase, lipase and amylase activities were significantly influenced by diet and weight. Specific trypsin activity was significantly higher for crayfish fed with the HL diet. Trypsin activity depended on diet and weight. Protein digestibility showed that HL was the most digestible diet and RF the least. The weight of the animals did not affect protein digestibility. Structural disorganization, hypertrophy of B-cells and presence of large vacuoles in R-cells were mainly observed in juveniles fed with HL, indicative of malnutrition. Thus, our data suggest that the HL diet would not be the most appropriate for C. quadricarinatus, while RF diet would be more convenient for culture of this species. © 2015 The Royal Swedish Academy of Sciences Fil:Sacristán, H.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fernández-Gimenez, A.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00017272_v97_n4_p407_Sacristan http://hdl.handle.net/20.500.12110/paper_00017272_v97_n4_p407_Sacristan
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Cherax quadricarinatus digestive enzymes feed formulations in vitro protein digestibility midgut gland artificial diet crayfish digestibility enzyme activity food quality histology juvenile lipid malnutrition protein Animalia Astacoidea Cherax quadricarinatus
spellingShingle	Cherax quadricarinatus digestive enzymes feed formulations in vitro protein digestibility midgut gland artificial diet crayfish digestibility enzyme activity food quality histology juvenile lipid malnutrition protein Animalia Astacoidea Cherax quadricarinatus Sacristán, Hernán Javier Fernández Gimenez, Analia Verónica Effect of different diets on digestive enzyme activities, in vitro digestibility, and midgut gland structure in juvenile crayfish, Cherax quadricarinatus
topic_facet	Cherax quadricarinatus digestive enzymes feed formulations in vitro protein digestibility midgut gland artificial diet crayfish digestibility enzyme activity food quality histology juvenile lipid malnutrition protein Animalia Astacoidea Cherax quadricarinatus
description	This study investigated the effects of food quality on digestive enzyme activities, in vitro protein digestibility and histological traits of the midgut gland in juvenile crayfish Cherax quadricarinatus. Animals of a wide weight range were fed different diets: two commercial diets with high or low lipid content (high lipid and low lipid, respectively) and were compared with a reference diet (RF) previously formulated for this species. Proteinase, lipase and amylase activities were significantly influenced by diet and weight. Specific trypsin activity was significantly higher for crayfish fed with the HL diet. Trypsin activity depended on diet and weight. Protein digestibility showed that HL was the most digestible diet and RF the least. The weight of the animals did not affect protein digestibility. Structural disorganization, hypertrophy of B-cells and presence of large vacuoles in R-cells were mainly observed in juveniles fed with HL, indicative of malnutrition. Thus, our data suggest that the HL diet would not be the most appropriate for C. quadricarinatus, while RF diet would be more convenient for culture of this species. © 2015 The Royal Swedish Academy of Sciences
author	Sacristán, Hernán Javier Fernández Gimenez, Analia Verónica
author_facet	Sacristán, Hernán Javier Fernández Gimenez, Analia Verónica
author_sort	Sacristán, Hernán Javier
title	Effect of different diets on digestive enzyme activities, in vitro digestibility, and midgut gland structure in juvenile crayfish, Cherax quadricarinatus
title_short	Effect of different diets on digestive enzyme activities, in vitro digestibility, and midgut gland structure in juvenile crayfish, Cherax quadricarinatus
title_full	Effect of different diets on digestive enzyme activities, in vitro digestibility, and midgut gland structure in juvenile crayfish, Cherax quadricarinatus
title_fullStr	Effect of different diets on digestive enzyme activities, in vitro digestibility, and midgut gland structure in juvenile crayfish, Cherax quadricarinatus
title_full_unstemmed	Effect of different diets on digestive enzyme activities, in vitro digestibility, and midgut gland structure in juvenile crayfish, Cherax quadricarinatus
title_sort	effect of different diets on digestive enzyme activities, in vitro digestibility, and midgut gland structure in juvenile crayfish, cherax quadricarinatus
publishDate	2016
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00017272_v97_n4_p407_Sacristan http://hdl.handle.net/20.500.12110/paper_00017272_v97_n4_p407_Sacristan
work_keys_str_mv	AT sacristanhernanjavier effectofdifferentdietsondigestiveenzymeactivitiesinvitrodigestibilityandmidgutglandstructureinjuvenilecrayfishcheraxquadricarinatus AT fernandezgimenezanaliaveronica effectofdifferentdietsondigestiveenzymeactivitiesinvitrodigestibilityandmidgutglandstructureinjuvenilecrayfishcheraxquadricarinatus
_version_	1768543012036542464

Effect of different diets on digestive enzyme activities, in vitro digestibility, and midgut gland structure in juvenile crayfish, Cherax quadricarinatus

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