Detection of Trypanosoma cruzi infection in naturally infected dogs and cats using serological, parasitological and molecular methods
Domestic dogs and cats are major domestic reservoir hosts of Trypanosoma cruzi and a risk factor for parasite transmission. In this study we assessed the relative performance of a polymerase chain reaction assay targeted to minicircle DNA (kDNA-PCR) in reference to conventional serological tests, a...
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2013
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0001706X_v126_n3_p211_Enriquez http://hdl.handle.net/20.500.12110/paper_0001706X_v126_n3_p211_Enriquez |
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paper:paper_0001706X_v126_n3_p211_Enriquez2023-06-08T14:21:08Z Detection of Trypanosoma cruzi infection in naturally infected dogs and cats using serological, parasitological and molecular methods Chagas disease Polymerase chain reaction Reservoir Serodiagnosis Trypanosoma cruzi Xenodiagnosis minicircle DNA canid detection method disease incidence DNA felid immunoassay infectivity parasite transmission polymerase chain reaction risk factor rural area trypanosomiasis animal experiment Argentina article cat disease Chagas disease controlled study cross-sectional study diagnostic test accuracy study dog disease enzyme linked immunosorbent assay hemagglutination test indirect hemagglutination assay intermethod comparison molecular diagnosis nonhuman parasite serodiagnosis polymerase chain reaction rapid dipstick test rural area sensitivity analysis Trypanosoma cruzi xenodiagnosis Animals Argentina Cat Diseases Cats Chagas Disease Diagnostic Tests, Routine Dog Diseases Dogs Molecular Diagnostic Techniques Parasitology Sensitivity and Specificity Serologic Tests Trypanosoma cruzi Veterinary Medicine Argentina Canis familiaris Trypanosoma cruzi Domestic dogs and cats are major domestic reservoir hosts of Trypanosoma cruzi and a risk factor for parasite transmission. In this study we assessed the relative performance of a polymerase chain reaction assay targeted to minicircle DNA (kDNA-PCR) in reference to conventional serological tests, a rapid dipstick test and xenodiagnosis to detect T. cruzi infection in dogs and cats from an endemic rural area in northeastern Argentina. A total of 43 dogs and 13 cats seropositive for T. cruzi by an immunosorbent assay (ELISA) and an indirect hemagglutination assay (IHA), which had been examined by xenodiagnosis, were also tested by kDNA-PCR. kDNA-PCR was nearly as sensitive as xenodiagnosis for detecting T. cruzi-infectious dogs and cats. kDNA-PCR was slightly more sensitive than xenodiagnosis in seropositive dogs (91% versus 86%, respectively) and cats (77% against 54%, respectively), but failed to detect all of the seropositive individuals. ELISA and IHA detected all xenodiagnosis-positive dogs and both outcomes largely agreed (kappa coefficient, κ= 0.92), whereas both assays failed to detect all of the xenodiagnosis-positive cats and their agreement was moderate (κ= 0.68). In dogs, the sensitivity of the dipstick test was 95% and agreed closely with the outcome of conventional serological tests (κ= 0.82). The high sensitivity of kDNA-PCR to detect T. cruzi infections in naturally infected dogs and cats supports its application as a diagnostic tool complementary to serology and may replace the use of xenodiagnosis or hemoculture. © 2013 Elsevier B.V. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0001706X_v126_n3_p211_Enriquez http://hdl.handle.net/20.500.12110/paper_0001706X_v126_n3_p211_Enriquez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Chagas disease Polymerase chain reaction Reservoir Serodiagnosis Trypanosoma cruzi Xenodiagnosis minicircle DNA canid detection method disease incidence DNA felid immunoassay infectivity parasite transmission polymerase chain reaction risk factor rural area trypanosomiasis animal experiment Argentina article cat disease Chagas disease controlled study cross-sectional study diagnostic test accuracy study dog disease enzyme linked immunosorbent assay hemagglutination test indirect hemagglutination assay intermethod comparison molecular diagnosis nonhuman parasite serodiagnosis polymerase chain reaction rapid dipstick test rural area sensitivity analysis Trypanosoma cruzi xenodiagnosis Animals Argentina Cat Diseases Cats Chagas Disease Diagnostic Tests, Routine Dog Diseases Dogs Molecular Diagnostic Techniques Parasitology Sensitivity and Specificity Serologic Tests Trypanosoma cruzi Veterinary Medicine Argentina Canis familiaris Trypanosoma cruzi |
| spellingShingle |
Chagas disease Polymerase chain reaction Reservoir Serodiagnosis Trypanosoma cruzi Xenodiagnosis minicircle DNA canid detection method disease incidence DNA felid immunoassay infectivity parasite transmission polymerase chain reaction risk factor rural area trypanosomiasis animal experiment Argentina article cat disease Chagas disease controlled study cross-sectional study diagnostic test accuracy study dog disease enzyme linked immunosorbent assay hemagglutination test indirect hemagglutination assay intermethod comparison molecular diagnosis nonhuman parasite serodiagnosis polymerase chain reaction rapid dipstick test rural area sensitivity analysis Trypanosoma cruzi xenodiagnosis Animals Argentina Cat Diseases Cats Chagas Disease Diagnostic Tests, Routine Dog Diseases Dogs Molecular Diagnostic Techniques Parasitology Sensitivity and Specificity Serologic Tests Trypanosoma cruzi Veterinary Medicine Argentina Canis familiaris Trypanosoma cruzi Detection of Trypanosoma cruzi infection in naturally infected dogs and cats using serological, parasitological and molecular methods |
| topic_facet |
Chagas disease Polymerase chain reaction Reservoir Serodiagnosis Trypanosoma cruzi Xenodiagnosis minicircle DNA canid detection method disease incidence DNA felid immunoassay infectivity parasite transmission polymerase chain reaction risk factor rural area trypanosomiasis animal experiment Argentina article cat disease Chagas disease controlled study cross-sectional study diagnostic test accuracy study dog disease enzyme linked immunosorbent assay hemagglutination test indirect hemagglutination assay intermethod comparison molecular diagnosis nonhuman parasite serodiagnosis polymerase chain reaction rapid dipstick test rural area sensitivity analysis Trypanosoma cruzi xenodiagnosis Animals Argentina Cat Diseases Cats Chagas Disease Diagnostic Tests, Routine Dog Diseases Dogs Molecular Diagnostic Techniques Parasitology Sensitivity and Specificity Serologic Tests Trypanosoma cruzi Veterinary Medicine Argentina Canis familiaris Trypanosoma cruzi |
| description |
Domestic dogs and cats are major domestic reservoir hosts of Trypanosoma cruzi and a risk factor for parasite transmission. In this study we assessed the relative performance of a polymerase chain reaction assay targeted to minicircle DNA (kDNA-PCR) in reference to conventional serological tests, a rapid dipstick test and xenodiagnosis to detect T. cruzi infection in dogs and cats from an endemic rural area in northeastern Argentina. A total of 43 dogs and 13 cats seropositive for T. cruzi by an immunosorbent assay (ELISA) and an indirect hemagglutination assay (IHA), which had been examined by xenodiagnosis, were also tested by kDNA-PCR. kDNA-PCR was nearly as sensitive as xenodiagnosis for detecting T. cruzi-infectious dogs and cats. kDNA-PCR was slightly more sensitive than xenodiagnosis in seropositive dogs (91% versus 86%, respectively) and cats (77% against 54%, respectively), but failed to detect all of the seropositive individuals. ELISA and IHA detected all xenodiagnosis-positive dogs and both outcomes largely agreed (kappa coefficient, κ= 0.92), whereas both assays failed to detect all of the xenodiagnosis-positive cats and their agreement was moderate (κ= 0.68). In dogs, the sensitivity of the dipstick test was 95% and agreed closely with the outcome of conventional serological tests (κ= 0.82). The high sensitivity of kDNA-PCR to detect T. cruzi infections in naturally infected dogs and cats supports its application as a diagnostic tool complementary to serology and may replace the use of xenodiagnosis or hemoculture. © 2013 Elsevier B.V. |
| title |
Detection of Trypanosoma cruzi infection in naturally infected dogs and cats using serological, parasitological and molecular methods |
| title_short |
Detection of Trypanosoma cruzi infection in naturally infected dogs and cats using serological, parasitological and molecular methods |
| title_full |
Detection of Trypanosoma cruzi infection in naturally infected dogs and cats using serological, parasitological and molecular methods |
| title_fullStr |
Detection of Trypanosoma cruzi infection in naturally infected dogs and cats using serological, parasitological and molecular methods |
| title_full_unstemmed |
Detection of Trypanosoma cruzi infection in naturally infected dogs and cats using serological, parasitological and molecular methods |
| title_sort |
detection of trypanosoma cruzi infection in naturally infected dogs and cats using serological, parasitological and molecular methods |
| publishDate |
2013 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0001706X_v126_n3_p211_Enriquez http://hdl.handle.net/20.500.12110/paper_0001706X_v126_n3_p211_Enriquez |
| _version_ |
1768542912708083712 |