Action of KI and several iodocompounds on [3H]uridine incorporation into thyroid RNA

The present studies were performed in order to further clarify the action of iodine and iodocompounds on the incorporation of labelled uridine into thyroid RNA. KI decreased RNA labelling but did not alter total (3H) uridine uptake or (3H) inulin distribution space. KI also inhibited the increase in...

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Publicado:	1978
Materias:	acetic acid derivative iodine derivative liothyronine potassium iodide radioisotope rna thyroxine 3 isopropyl 5,3,5 triiodothyronine 3,5 diiodo 3' isopropylthyronine animal experiment cattle endocrine system in vitro study inulin h 3 rna synthesis thyroid gland triiodoacetic acid uridine h 3 Animal Cattle Cyclic AMP Cyclic GMP Glucose Inulin Iodides Iodoacetates Potassium Iodide RNA Thyroid Gland Thyroxine Triiodothyronine, Reverse Tritium Uridine
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00015598_v89_n2_p316_KleimanDePisarev http://hdl.handle.net/20.500.12110/paper_00015598_v89_n2_p316_KleimanDePisarev
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00015598_v89_n2_p316_KleimanDePisarev
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spelling	paper:paper_00015598_v89_n2_p316_KleimanDePisarev2023-06-08T14:20:54Z Action of KI and several iodocompounds on [3H]uridine incorporation into thyroid RNA acetic acid derivative iodine derivative liothyronine potassium iodide radioisotope rna thyroxine 3 isopropyl 5,3,5 triiodothyronine 3,5 diiodo 3' isopropylthyronine animal experiment cattle endocrine system in vitro study inulin h 3 rna synthesis thyroid gland triiodoacetic acid uridine h 3 Animal Cattle Cyclic AMP Cyclic GMP Glucose Inulin Iodides Iodoacetates Potassium Iodide RNA Thyroid Gland Thyroxine Triiodothyronine, Reverse Tritium Uridine The present studies were performed in order to further clarify the action of iodine and iodocompounds on the incorporation of labelled uridine into thyroid RNA. KI decreased RNA labelling but did not alter total (3H) uridine uptake or (3H) inulin distribution space. KI also inhibited the increase in RNA labelling produced by 8mM glucose. T4 was more potent on a molar basis than KI in impairing uridine incorporation. TETRAC,TRIAC and isopropyl-T3 also decreased RNA labelling, while T2 and isopropyl-T2 were ineffective. KI did not alter the distribution of the uridine derivatives, UMP,UDP and UTP, as determined by the distribution of (3H) uridine in these compounds by paper chromatography, suggesting that the action of KI does not take place at the step of uridine phosphorylation. Like its effect on TSH, KI also impairs the stimulatory effect of exogenous cAMP and cGMP on RNA labelling, suggesting that its action is exerted beyond the step of cyclic nucleotide formation. Iodine and iodocompounds may exert their inhibitory action on RNA labelling at the step of nucleotide polymerization. 1978 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00015598_v89_n2_p316_KleimanDePisarev http://hdl.handle.net/20.500.12110/paper_00015598_v89_n2_p316_KleimanDePisarev
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	acetic acid derivative iodine derivative liothyronine potassium iodide radioisotope rna thyroxine 3 isopropyl 5,3,5 triiodothyronine 3,5 diiodo 3' isopropylthyronine animal experiment cattle endocrine system in vitro study inulin h 3 rna synthesis thyroid gland triiodoacetic acid uridine h 3 Animal Cattle Cyclic AMP Cyclic GMP Glucose Inulin Iodides Iodoacetates Potassium Iodide RNA Thyroid Gland Thyroxine Triiodothyronine, Reverse Tritium Uridine
spellingShingle	acetic acid derivative iodine derivative liothyronine potassium iodide radioisotope rna thyroxine 3 isopropyl 5,3,5 triiodothyronine 3,5 diiodo 3' isopropylthyronine animal experiment cattle endocrine system in vitro study inulin h 3 rna synthesis thyroid gland triiodoacetic acid uridine h 3 Animal Cattle Cyclic AMP Cyclic GMP Glucose Inulin Iodides Iodoacetates Potassium Iodide RNA Thyroid Gland Thyroxine Triiodothyronine, Reverse Tritium Uridine Action of KI and several iodocompounds on [3H]uridine incorporation into thyroid RNA
topic_facet	acetic acid derivative iodine derivative liothyronine potassium iodide radioisotope rna thyroxine 3 isopropyl 5,3,5 triiodothyronine 3,5 diiodo 3' isopropylthyronine animal experiment cattle endocrine system in vitro study inulin h 3 rna synthesis thyroid gland triiodoacetic acid uridine h 3 Animal Cattle Cyclic AMP Cyclic GMP Glucose Inulin Iodides Iodoacetates Potassium Iodide RNA Thyroid Gland Thyroxine Triiodothyronine, Reverse Tritium Uridine
description	The present studies were performed in order to further clarify the action of iodine and iodocompounds on the incorporation of labelled uridine into thyroid RNA. KI decreased RNA labelling but did not alter total (3H) uridine uptake or (3H) inulin distribution space. KI also inhibited the increase in RNA labelling produced by 8mM glucose. T4 was more potent on a molar basis than KI in impairing uridine incorporation. TETRAC,TRIAC and isopropyl-T3 also decreased RNA labelling, while T2 and isopropyl-T2 were ineffective. KI did not alter the distribution of the uridine derivatives, UMP,UDP and UTP, as determined by the distribution of (3H) uridine in these compounds by paper chromatography, suggesting that the action of KI does not take place at the step of uridine phosphorylation. Like its effect on TSH, KI also impairs the stimulatory effect of exogenous cAMP and cGMP on RNA labelling, suggesting that its action is exerted beyond the step of cyclic nucleotide formation. Iodine and iodocompounds may exert their inhibitory action on RNA labelling at the step of nucleotide polymerization.
title	Action of KI and several iodocompounds on [3H]uridine incorporation into thyroid RNA
title_short	Action of KI and several iodocompounds on [3H]uridine incorporation into thyroid RNA
title_full	Action of KI and several iodocompounds on [3H]uridine incorporation into thyroid RNA
title_fullStr	Action of KI and several iodocompounds on [3H]uridine incorporation into thyroid RNA
title_full_unstemmed	Action of KI and several iodocompounds on [3H]uridine incorporation into thyroid RNA
title_sort	action of ki and several iodocompounds on [3h]uridine incorporation into thyroid rna
publishDate	1978
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00015598_v89_n2_p316_KleimanDePisarev http://hdl.handle.net/20.500.12110/paper_00015598_v89_n2_p316_KleimanDePisarev
_version_	1768544156395765760

Action of KI and several iodocompounds on [3H]uridine incorporation into thyroid RNA

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