Evaluation of the South American gastropod Heleobia parchappii as test organism in cadmium toxicity bioassays

Mostrar todas las versiones(2)

Gastropods are abundant key components in freshwater ecosystems, many of which are affected by anthropogenic activities. The genus Heleobia is widely distributed across Argentina, and the native Heleobia parchappii is very common in the Pampean Region. The identification of native candidate species...

Descripción completa

Detalles Bibliográficos
Autores principales: Achiorno, Cecilia Lujan, Minardi, Graciela Mónica, Schneider, Marcela Inés, Fogel, Marilina Noelia
Formato: Artículo publishedVersion
Lenguaje:Inglés
Publicado: Asociación Argentina de Ecología 2023
Materias:
MOLUSCOS
ESPECIES NATIVAS
BIOENSAYOS
DULCEACUICOLA
TOXICIDAD AGUDA
CLORURO DE CADMIO
MOLLUSK
NATIVE SPECIES
BIOASSAY
FRESHWATER
ACUTE TOXICITY
CADMIUM CHLORIDE
Acceso en línea:https://hdl.handle.net/20.500.12110/ecologiaaustral_v033_n01_p188
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id ecologiaaustral:ecologiaaustral_v033_n01_p188
record_format dspace
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language Inglés
orig_language_str_mv eng
topic MOLUSCOS
ESPECIES NATIVAS
BIOENSAYOS
DULCEACUICOLA
TOXICIDAD AGUDA
CLORURO DE CADMIO
MOLLUSK
NATIVE SPECIES
BIOASSAY
FRESHWATER
ACUTE TOXICITY
CADMIUM CHLORIDE
spellingShingle MOLUSCOS
ESPECIES NATIVAS
BIOENSAYOS
DULCEACUICOLA
TOXICIDAD AGUDA
CLORURO DE CADMIO
MOLLUSK
NATIVE SPECIES
BIOASSAY
FRESHWATER
ACUTE TOXICITY
CADMIUM CHLORIDE
Achiorno, Cecilia Lujan
Minardi, Graciela Mónica
Schneider, Marcela Inés
Fogel, Marilina Noelia
Evaluation of the South American gastropod Heleobia parchappii as test organism in cadmium toxicity bioassays
topic_facet MOLUSCOS
ESPECIES NATIVAS
BIOENSAYOS
DULCEACUICOLA
TOXICIDAD AGUDA
CLORURO DE CADMIO
MOLLUSK
NATIVE SPECIES
BIOASSAY
FRESHWATER
ACUTE TOXICITY
CADMIUM CHLORIDE
description Gastropods are abundant key components in freshwater ecosystems, many of which are affected by anthropogenic activities. The genus Heleobia is widely distributed across Argentina, and the native Heleobia parchappii is very common in the Pampean Region. The identification of native candidate species for toxicity testing is gaining increasing interest. The objectives of the present study were to evaluate 1) the susceptibility of H. parchappii to a reference toxicant (Cl2 Cd), and 2) its suitability for toxicity testing by estimating LC50. Also, we discussed the applicability of the proposed protocol. Snail sensitivity was assessed by exposure to concentrations between 0.5 and 12 mg Cd/L under acute static conditions during 72 h (exposure period). Then, snails were transferred to the control medium for 24 h (post-exposure period) and checked for recovery and those not responding to stimulation were considered to be dead. The endpoints were snail immobilization (recorded every 24 h during the exposure period) and mortality at the end of the post-exposure period. Snail sensitivity was analyzed with a generalized linear mixed model (GLMM) and LC50 (lethal concentration 50%) was calculated using probit analysis. Results indicated that the number of immobilized snails increased with increasing concentration. The toxic effect of Cd on snails persisted 24 h after exposure. The LC50 was 2.145 (1.675-2.641) mg Cd/L. The sensitivity of H. parchappii was similar to that reported for other freshwater standard species, supporting its suitability as test organism. The protocol was appropriate for use in toxicity testing as it is simple, inexpensive and reproducible.
format Artículo
Artículo
publishedVersion
author Achiorno, Cecilia Lujan
Minardi, Graciela Mónica
Schneider, Marcela Inés
Fogel, Marilina Noelia
author_facet Achiorno, Cecilia Lujan
Minardi, Graciela Mónica
Schneider, Marcela Inés
Fogel, Marilina Noelia
author_sort Achiorno, Cecilia Lujan
title Evaluation of the South American gastropod Heleobia parchappii as test organism in cadmium toxicity bioassays
title_short Evaluation of the South American gastropod Heleobia parchappii as test organism in cadmium toxicity bioassays
title_full Evaluation of the South American gastropod Heleobia parchappii as test organism in cadmium toxicity bioassays
title_fullStr Evaluation of the South American gastropod Heleobia parchappii as test organism in cadmium toxicity bioassays
title_full_unstemmed Evaluation of the South American gastropod Heleobia parchappii as test organism in cadmium toxicity bioassays
title_sort evaluation of the south american gastropod heleobia parchappii as test organism in cadmium toxicity bioassays
publisher Asociación Argentina de Ecología
publishDate 2023
url https://hdl.handle.net/20.500.12110/ecologiaaustral_v033_n01_p188
work_keys_str_mv AT achiornocecilialujan evaluationofthesouthamericangastropodheleobiaparchappiiastestorganismincadmiumtoxicitybioassays
AT minardigracielamonica evaluationofthesouthamericangastropodheleobiaparchappiiastestorganismincadmiumtoxicitybioassays
AT schneidermarcelaines evaluationofthesouthamericangastropodheleobiaparchappiiastestorganismincadmiumtoxicitybioassays
AT fogelmarilinanoelia evaluationofthesouthamericangastropodheleobiaparchappiiastestorganismincadmiumtoxicitybioassays
AT achiornocecilialujan evaluaciondelgasteropodosudamericanoheleobiaparchappiicomoorganismodiagnosticoenbioensayosdetoxicidadconcadmio
AT minardigracielamonica evaluaciondelgasteropodosudamericanoheleobiaparchappiicomoorganismodiagnosticoenbioensayosdetoxicidadconcadmio
AT schneidermarcelaines evaluaciondelgasteropodosudamericanoheleobiaparchappiicomoorganismodiagnosticoenbioensayosdetoxicidadconcadmio
AT fogelmarilinanoelia evaluaciondelgasteropodosudamericanoheleobiaparchappiicomoorganismodiagnosticoenbioensayosdetoxicidadconcadmio
_version_ 1831983443763789824
spelling ecologiaaustral:ecologiaaustral_v033_n01_p1882025-03-28T09:39:01Z Evaluation of the South American gastropod Heleobia parchappii as test organism in cadmium toxicity bioassays Evaluación del gasterópodo sudamericano Heleobia parchappii como organismo diagnóstico en bioensayos de toxicidad con cadmio Ecol. austral (En línea) 2023;01(033):188-197 Achiorno, Cecilia Lujan Minardi, Graciela Mónica Schneider, Marcela Inés Fogel, Marilina Noelia MOLUSCOS ESPECIES NATIVAS BIOENSAYOS DULCEACUICOLA TOXICIDAD AGUDA CLORURO DE CADMIO MOLLUSK NATIVE SPECIES BIOASSAY FRESHWATER ACUTE TOXICITY CADMIUM CHLORIDE Gastropods are abundant key components in freshwater ecosystems, many of which are affected by anthropogenic activities. The genus Heleobia is widely distributed across Argentina, and the native Heleobia parchappii is very common in the Pampean Region. The identification of native candidate species for toxicity testing is gaining increasing interest. The objectives of the present study were to evaluate 1) the susceptibility of H. parchappii to a reference toxicant (Cl2 Cd), and 2) its suitability for toxicity testing by estimating LC50. Also, we discussed the applicability of the proposed protocol. Snail sensitivity was assessed by exposure to concentrations between 0.5 and 12 mg Cd/L under acute static conditions during 72 h (exposure period). Then, snails were transferred to the control medium for 24 h (post-exposure period) and checked for recovery and those not responding to stimulation were considered to be dead. The endpoints were snail immobilization (recorded every 24 h during the exposure period) and mortality at the end of the post-exposure period. Snail sensitivity was analyzed with a generalized linear mixed model (GLMM) and LC50 (lethal concentration 50%) was calculated using probit analysis. Results indicated that the number of immobilized snails increased with increasing concentration. The toxic effect of Cd on snails persisted 24 h after exposure. The LC50 was 2.145 (1.675-2.641) mg Cd/L. The sensitivity of H. parchappii was similar to that reported for other freshwater standard species, supporting its suitability as test organism. The protocol was appropriate for use in toxicity testing as it is simple, inexpensive and reproducible. Los gasterópodos son componentes clave de los ecosistemas acuáticos, y muchos de ellos están afectados por la actividad antrópica. El género Heleobia está ampliamente distribuido en la Argentina, y la especie nativa Heleobia parchappii es muy común en la Región Pampeana. Actualmente, existe un interés creciente en identificar especies nativas para usarlas en evaluaciones de toxicidad. Los objetivos de este estudio fueron evaluar 1) la susceptibilidad de H. parchappii a un tóxico de referencia (Cl2 Cd), y 2) su idoneidad para ser utilizada en evaluaciones de toxicidad estimando la concentración letal 50% (CL50). Además, discutimos la aplicabilidad del protocolo propuesto. La sensibilidad de los caracoles fue evaluada al exponerlos a concentraciones entre 0.5 y 12 mg Cd/L en un ensayo agudo bajo condiciones estáticas durante 72 h (tiempo de exposición). Los caracoles se transfirieron a un medio control durante 24 h (período de post-exposición) y se controló su estado para determinar si se recuperaban; aquellos que no respondían a los estímulos se consideraron muertos. Los puntos finales evaluados fueron la inmovilización de los caracoles (registrada cada 24 h durante el período de exposición) y la mortalidad al finalizar el período de post-exposición. La sensibilidad de los caracoles se analizó utilizando un modelo lineal generalizado y se calculó la CL50 usando el análisis probit. Los resultados indican que el número de caracoles inmovilizados aumentó con el incremento de la concentración. El efecto tóxico del Cd sobre los caracoles persistió 24 h tras la exposición. La CL50 tuvo un valor de 2.145 (1.675-2.641) mg Cd/L. La sensibilidad de H. parchappii fue similar a la informada para otros especies estándar dulceacuícolas, lo que respalda su idoneidad como organismo de evaluación. El protocolo fue apropiado para su uso en evaluaciones de toxicidad ya que es simple, económico y reproducible. Fil: Achiorno, Cecilia Lujan. Universidad Nacional de La Plata - CONICET. Centro de Estudios Parasitológicos y de Vectores (CEPAVE) Fil: Minardi, Graciela Mónica. Universidad Nacional de La Plata - CONICET. Centro de Estudios Parasitológicos y de Vectores (CEPAVE) Fil: Schneider, Marcela Inés. Universidad Nacional de La Plata - CONICET. Centro de Estudios Parasitológicos y de Vectores (CEPAVE) Fil: Fogel, Marilina Noelia. Universidad Nacional de La Plata - CONICET. Centro de Estudios Parasitológicos y de Vectores (CEPAVE) Asociación Argentina de Ecología 2023-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar https://hdl.handle.net/20.500.12110/ecologiaaustral_v033_n01_p188

Ejemplares similares