Forensic identification of a tapir (Tapirus terrestris) from a roadkill crime, using mitochondrial and nuclear markers

Fil: Sotorres, Delfina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Subtropical; Argentina.

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Autores principales: Sotorres, Delfina, Ferreyra, Analía Mercedes, DeMatteo, Karen Elizabeth, Argüelles, Carina Francisca
Formato: Documento de conferencia publishedVersion
Lenguaje:Inglés
Publicado: 2020
Materias:
Tapirus terrestris
Molecular markers
Wildlife DNA forensics
Acceso en línea:https://hdl.handle.net/20.500.12219/5375
Aporte de:
Repositorio Institucional Digital de la UNaM (RIDUNAM) de Universidad Nacional de Misiones
id I77-R195-20.500.12219-5375
record_format dspace
spelling I77-R195-20.500.12219-53752025-01-29T12:16:48Z Forensic identification of a tapir (Tapirus terrestris) from a roadkill crime, using mitochondrial and nuclear markers Sotorres, Delfina Ferreyra, Analía Mercedes DeMatteo, Karen Elizabeth Argüelles, Carina Francisca Tapirus terrestris Molecular markers Wildlife DNA forensics Fil: Sotorres, Delfina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Subtropical; Argentina. Fil: Sotorres, Delfina. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina. Fil: Sotorres, Delfina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Genética. Laboratorio GIGA; Argentina. Fil: Ferreyra, Analía Mercedes. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Genética. Laboratorio GIGA; Argentina. Fil: DeMatteo, Karen Elizabeth. Washington University in Saint Louis. Department of Biology & Environmental Studies (Missouri); Argentina. Fil: DeMatteo, Karen Elizabeth. WildCare Institute at the Saint Louis Zoo (Missouri); Argentina. Fil: Argüelles, Carina Francisca. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Subtropical; Argentina. Fil: Argüelles, Carina Francisca. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biología Subtropical; Argentina. Fil: Argüelles, Carina Francisca. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Genética. Laboratorio GIGA; Argentina. The province of Misiones in Argentina contains the largest remnant of the Atlantic Forest Ecoregion, a world biodiversity hotspot. However, this remnant is fragmented and suffers human-wildlife conflict, including road kills; both threaten wildlife. Presented, is a forensic case that involved a hit-by-vehicle (HBV) tapir (Tapirus terrestris), a Natural Monument that receives the highest legal protection in Misiones, on the 31 March 2019 in northern Misiones. While the driver involved in the crash left the scene, he was subsequently found in a nearby town. However, he claimed that he hit a horse, not a tapir, with his truck. Genetic analysis of evidence from his truck versus the reference HBV tapir was requested in order to clarify the legal case. DNA was extracted from the HBV tapir (muscular tissue) and vehicle evidence (hair and blood swabs) following a phenol-chloroform protocol. For species identification, a portion of the mitochondrial Cytb gene was amplified. For individual genotyping, 12 sets of microsatellites (STRs) were amplified. All genetic protocols had been previously optimized for tapir. Ctyb gene of the HBV tapir and vehicle evidence had a 100% overlap in their genetic sequences, indicating the driver hit a tapir, not a horse. A match was also found across the STR alleles amplified, between evidence and reference samples. The forensic report was sent to the requesting authorities for use in their investigation and legal efforts. This work demonstrates the power of wildlife forensic analyses in assisting with the enforcement of conservation laws. 2020-11-09 info:eu-repo/semantics/conferenceObject info:ar-repo/semantics/documento de conferencia info:eu-repo/semantics/publishedVersion https://hdl.handle.net/20.500.12219/5375 eng info:eu-repo/semantics/openAccess application/pdf application/pdf 147.0 KB
institution Universidad Nacional de Misiones
institution_str I-77
repository_str R-195
collection Repositorio Institucional Digital de la UNaM (RIDUNAM)
language Inglés
topic Tapirus terrestris
Molecular markers
Wildlife DNA forensics
spellingShingle Tapirus terrestris
Molecular markers
Wildlife DNA forensics
Sotorres, Delfina
Ferreyra, Analía Mercedes
DeMatteo, Karen Elizabeth
Argüelles, Carina Francisca
Forensic identification of a tapir (Tapirus terrestris) from a roadkill crime, using mitochondrial and nuclear markers
topic_facet Tapirus terrestris
Molecular markers
Wildlife DNA forensics
description Fil: Sotorres, Delfina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Subtropical; Argentina.
format Documento de conferencia
Documento de conferencia
publishedVersion
author Sotorres, Delfina
Ferreyra, Analía Mercedes
DeMatteo, Karen Elizabeth
Argüelles, Carina Francisca
author_facet Sotorres, Delfina
Ferreyra, Analía Mercedes
DeMatteo, Karen Elizabeth
Argüelles, Carina Francisca
author_sort Sotorres, Delfina
title Forensic identification of a tapir (Tapirus terrestris) from a roadkill crime, using mitochondrial and nuclear markers
title_short Forensic identification of a tapir (Tapirus terrestris) from a roadkill crime, using mitochondrial and nuclear markers
title_full Forensic identification of a tapir (Tapirus terrestris) from a roadkill crime, using mitochondrial and nuclear markers
title_fullStr Forensic identification of a tapir (Tapirus terrestris) from a roadkill crime, using mitochondrial and nuclear markers
title_full_unstemmed Forensic identification of a tapir (Tapirus terrestris) from a roadkill crime, using mitochondrial and nuclear markers
title_sort forensic identification of a tapir (tapirus terrestris) from a roadkill crime, using mitochondrial and nuclear markers
publishDate 2020
url https://hdl.handle.net/20.500.12219/5375
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