Selection of Trichoderma strain to enhanced cellulase-poor xylanase production using sugarcane bagasse as sole carbon source under light

Fil: Barchuk, Mónica Lucrecia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.

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Autores principales: Barchuk, Mónica Lucrecia, Díaz, Gabriela Verónica, Coll, Pedro Agustín Francisco, Velázquez, Juan Ernesto, Fonseca, María Isabel, Villalba, Laura Lidia, Zapata, Pedro Darío
Formato: Artículo publishedVersion
Lenguaje:Inglés
Publicado: Vital Biotech (India) 2016
Materias:
Trichoderma
Xylanases
Sugarcane bagasse (SCB)
Light
Acceso en línea:https://hdl.handle.net/20.500.12219/4452
Aporte de:
Repositorio Institucional Digital de la UNaM (RIDUNAM) de Universidad Nacional de Misiones
id I77-R195-20.500.12219-4452
record_format dspace
spelling I77-R195-20.500.12219-44522024-06-04T11:37:58Z Selection of Trichoderma strain to enhanced cellulase-poor xylanase production using sugarcane bagasse as sole carbon source under light Barchuk, Mónica Lucrecia Díaz, Gabriela Verónica Coll, Pedro Agustín Francisco Velázquez, Juan Ernesto Fonseca, María Isabel Villalba, Laura Lidia Zapata, Pedro Darío Trichoderma Xylanases Sugarcane bagasse (SCB) Light Fil: Barchuk, Mónica Lucrecia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina. Fil: Díaz, Gabriela Verónica. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina. Fil: Coll, Pedro Agustín Francisco. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina. Fil: Velázquez, Juan Ernesto. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina. Fil: Fonseca, María Isabel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina. Fil: Zapata, Pedro Darío. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina. Fil: Villalba, Laura Lidia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina. Species from fungal genus Trichoderma were found to produce culture broths rich in xylanase activity, an enzyme which plays an essential role for efficient and complete hydrolysis of lignocellulosic biomass. This research aimed to enhance cellulase-poor xylanase production using a low-cost medium. Firstly, several native Trichoderma strains isolated from subtropical environments of Misiones (Argentina) were evaluated based on its xylanase producing potential. Among these, T. atroviride LBM 117 produced the highest cellulase-poor xylanase enzymes, 29 U mL-1 and 28 U mL1 in experiment with and without light, respectively, using sugarcane bagasse as a sole carbon source. Besides, when experiments were carried out with light, the highest xylanase production occurred in shorter incubation period than experiments without it. Xylanase activity was characterized in both supernatants showing the maximum xylanase activity at 50 °C and pH 4.8. Results of electrophoresis on polyacrylamide gels showed two isoenzymes of 105 and 150 KDa in supernatants from experiment with light and only one isoenzyme of 105 KDa from experiment in darkness. 2016-05-01 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://hdl.handle.net/20.500.12219/4452 eng info:eu-repo/semantics/altIdentifier/urn/https://www.researchgate.net/profile/Gabriela_Diaz40/publication/335099499_Selection_of_Trichoderma_Strain_to_Enhanced_Cellulase-Poor_Xylanase_Production_Using_Sugarcane_Bagasse_as_Sole_Carbon_Source_under_Light/links/5d4ed57da6fdcc370a8a67e4/Selection-of-Trichoderma-Strain-to-Enhanced-Cellulase-Poor-Xylanase-Production-Using-Sugarcane-Bagasse-as-Sole-Carbon-Source-under-Light.pdf info:eu-repo/semantics/openAccess application/pdf application/pdf 1.614 MB Vital Biotech (India)
institution Universidad Nacional de Misiones
institution_str I-77
repository_str R-195
collection Repositorio Institucional Digital de la UNaM (RIDUNAM)
language Inglés
topic Trichoderma
Xylanases
Sugarcane bagasse (SCB)
Light
spellingShingle Trichoderma
Xylanases
Sugarcane bagasse (SCB)
Light
Barchuk, Mónica Lucrecia
Díaz, Gabriela Verónica
Coll, Pedro Agustín Francisco
Velázquez, Juan Ernesto
Fonseca, María Isabel
Villalba, Laura Lidia
Zapata, Pedro Darío
Selection of Trichoderma strain to enhanced cellulase-poor xylanase production using sugarcane bagasse as sole carbon source under light
topic_facet Trichoderma
Xylanases
Sugarcane bagasse (SCB)
Light
description Fil: Barchuk, Mónica Lucrecia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
format Artículo
Artículo
publishedVersion
author Barchuk, Mónica Lucrecia
Díaz, Gabriela Verónica
Coll, Pedro Agustín Francisco
Velázquez, Juan Ernesto
Fonseca, María Isabel
Villalba, Laura Lidia
Zapata, Pedro Darío
author_facet Barchuk, Mónica Lucrecia
Díaz, Gabriela Verónica
Coll, Pedro Agustín Francisco
Velázquez, Juan Ernesto
Fonseca, María Isabel
Villalba, Laura Lidia
Zapata, Pedro Darío
author_sort Barchuk, Mónica Lucrecia
title Selection of Trichoderma strain to enhanced cellulase-poor xylanase production using sugarcane bagasse as sole carbon source under light
title_short Selection of Trichoderma strain to enhanced cellulase-poor xylanase production using sugarcane bagasse as sole carbon source under light
title_full Selection of Trichoderma strain to enhanced cellulase-poor xylanase production using sugarcane bagasse as sole carbon source under light
title_fullStr Selection of Trichoderma strain to enhanced cellulase-poor xylanase production using sugarcane bagasse as sole carbon source under light
title_full_unstemmed Selection of Trichoderma strain to enhanced cellulase-poor xylanase production using sugarcane bagasse as sole carbon source under light
title_sort selection of trichoderma strain to enhanced cellulase-poor xylanase production using sugarcane bagasse as sole carbon source under light
publisher Vital Biotech (India)
publishDate 2016
url https://hdl.handle.net/20.500.12219/4452
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