Effect of Debaryomyces hansenii and the antifungal PgAFP protein on Alternaria spp. growth, toxin production, and RHO1 gene expression in a tomato-based medium

Tomato fruit is susceptible to Alternaria spp. spoilage, which poses a health risk due to their mycotoxin production. Biopreservation relies on the use of whole microorganisms or their metabolites to manage spoilage microorganisms including filamentous fungi. However, the use of treatments at fungis...

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Autores principales: da Cruz Cabral, Lucía Mariana, Rodríguez, Alicia, Andrade, María J., Patriarca, Andrea, Delgado, Josué
Formato: Artículo acceptedVersion
Lenguaje:Inglés
Inglés
Publicado: Elsevier 2022
Materias:
Alternaria
Mycotoxin
Tomato fruit
Yeasts
Antifungal protein
Food safety
Acceso en línea:http://hdl.handle.net/20.500.12272/6975
https://doi.org/10.1016/j.fm.2021.103741
Aporte de:
RIA - Repositorio Institucional Abierto (UTN) de Universidad Tecnológica Nacional
id I68-R174-20.500.12272-6975
record_format dspace
institution Universidad Tecnológica Nacional
institution_str I-68
repository_str R-174
collection RIA - Repositorio Institucional Abierto (UTN)
language Inglés
Inglés
topic Alternaria
Mycotoxin
Tomato fruit
Yeasts
Antifungal protein
Food safety
spellingShingle Alternaria
Mycotoxin
Tomato fruit
Yeasts
Antifungal protein
Food safety
da Cruz Cabral, Lucía Mariana
Rodríguez, Alicia
Andrade, María J.
Patriarca, Andrea
Delgado, Josué
Effect of Debaryomyces hansenii and the antifungal PgAFP protein on Alternaria spp. growth, toxin production, and RHO1 gene expression in a tomato-based medium
topic_facet Alternaria
Mycotoxin
Tomato fruit
Yeasts
Antifungal protein
Food safety
description Tomato fruit is susceptible to Alternaria spp. spoilage, which poses a health risk due to their mycotoxin production. Biopreservation relies on the use of whole microorganisms or their metabolites to manage spoilage microorganisms including filamentous fungi. However, the use of treatments at fungistatic level might activate intracellular pathways, which can cause an increment in mycotoxin accumulation. The objective of this work was to evaluate the effect of two strains of Debaryomyces hansenii and the antifungal protein PgAFP at 10 and 40 μg/mL. Both growth and production of two of the most common mycotoxins (tenuazonic acid and alternariol monomethyl ether) by Alternaria tenuissima sp.-grp. and Alternaria arborescens sp.-grp. on a tomato-based matrix, were analysed at 12 °C. Additionally, the impact of these biocontrol agents on the stress-related RHO1 gene expression was assessed. All treatments reduced mycotoxin accumulation (from 27 to 92% of inhibition). Their mode of action against Alternaria spp. in tomato seems unrelated to damages to fungal cell wall integrity at the genomic level. Therefore, the two D. hansenii strains (CECT 10352 and CECT 10353) and the antifungal protein PgAFP at 10 μg/mL are suggested as biocontrol strategies in tomato fruit at postharvest stage.
format Artículo
acceptedVersion
author da Cruz Cabral, Lucía Mariana
Rodríguez, Alicia
Andrade, María J.
Patriarca, Andrea
Delgado, Josué
author_facet da Cruz Cabral, Lucía Mariana
Rodríguez, Alicia
Andrade, María J.
Patriarca, Andrea
Delgado, Josué
author_sort da Cruz Cabral, Lucía Mariana
title Effect of Debaryomyces hansenii and the antifungal PgAFP protein on Alternaria spp. growth, toxin production, and RHO1 gene expression in a tomato-based medium
title_short Effect of Debaryomyces hansenii and the antifungal PgAFP protein on Alternaria spp. growth, toxin production, and RHO1 gene expression in a tomato-based medium
title_full Effect of Debaryomyces hansenii and the antifungal PgAFP protein on Alternaria spp. growth, toxin production, and RHO1 gene expression in a tomato-based medium
title_fullStr Effect of Debaryomyces hansenii and the antifungal PgAFP protein on Alternaria spp. growth, toxin production, and RHO1 gene expression in a tomato-based medium
title_full_unstemmed Effect of Debaryomyces hansenii and the antifungal PgAFP protein on Alternaria spp. growth, toxin production, and RHO1 gene expression in a tomato-based medium
title_sort effect of debaryomyces hansenii and the antifungal pgafp protein on alternaria spp. growth, toxin production, and rho1 gene expression in a tomato-based medium
publisher Elsevier
publishDate 2022
url http://hdl.handle.net/20.500.12272/6975
https://doi.org/10.1016/j.fm.2021.103741
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