Secondary metabolites of Bougainvillea spinosa with antioxidant activity through callus grown in vitro under physical stress

Fil: Dalzotto, Daniela Cecilia. Universidad Nacional de Río Negro. Río Negro; Argentina

Detalles Bibliográficos
Autores principales: Dalzotto, Daniela Cecilia, Espíndola, Micaela, Huenelaf, Valeria Belén, Piñuel, María Lucrecia, Sharry, Sandra Elizabeth, Boeri, Patricia Alejandra
Lenguaje:Inglés
Publicado: 2021
Materias:
Biotecnología Agraria (General)
Elicitation
Bioactive compounds
ABTS
DPPH
Native flora
Acceso en línea:http://rid.unrn.edu.ar/handle/20.500.12049/10287
Aporte de:
Repositorio Institucional Digital de la Universidad Nacional de Río Negro (UNRN) de Universidad Nacional de Río Negro
id I65-R171-20.500.12049-10287
record_format dspace
spelling I65-R171-20.500.12049-102872023-06-01T12:03:49Z application/pdf info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/4.0/ 2021-09-17 Fil: Dalzotto, Daniela Cecilia. Universidad Nacional de Río Negro. Río Negro; Argentina Fil: Espíndola, Micaela. Universidad Nacional de Río Negro. Río Negro; Argentina Fil: Huenelaf, Micaela Belén. Universidad Nacional de Río Negro. Río Negro; Argentina Fil: Piñuel, María Lucrecia. Universidad Nacional de Río Negro. Río Negro; Argentina Fil: Sharry, Sandra Elizabeth. Universidad Nacional de Río Negro. Río Negro; Argentina Fil: Boeri, Patricia Alejandra. Universidad Nacional de Río Negro. Río Negro; Argentina Secondary metabolites of Bougainvillea spinosa with antioxidant activity through callus grown in vitro under physical stress Dalzotto, Daniela Cecilia Espíndola, Micaela Huenelaf, Valeria Belén Piñuel, María Lucrecia Sharry, Sandra Elizabeth Boeri, Patricia Alejandra Biotecnología Agraria (General) Elicitation Bioactive compounds ABTS DPPH Native flora Biotecnología Agraria (General) Plant tissue culture is an attractive alternative source of bioactive compounds, like secondary metabolites (SM). Elicitation by abiotic factors can be an important strategy towards improved in vitro production of these compounds. Bougainvillea spinosa is a woody species native to Argentina, which has been little studied in this aspect. The objective of this work was to evaluate if different physical stresses can induce the production of SM with antioxidant activity (AO) in B. espinosa callus obtained in vitro. The callus were laid in solid MS medium at half concentration with 30% sucrose for one month in two stress conditions: 1) saline stress with 150 µM NaCl; 2) UV light stress on the fifth day of culture. A control group was maintained in ½ MS. After culture, an extraction of the SM was carried out using 65% methanol as solvent. The AO of the extracts was measured through the DPPH and ABTS methods. The results showed that the stress induced by NaCl failed to elicit the synthesis of SM with AO in respect to the control group. However, extracts obtained from callus exposed to UV light stress increased AO in both methods (91.8±8 and 150.6±7 µmol troloxEQ/100 g fresh callus for DPPH and ABTS, respectively), with respect to the control (72.1±9 and 108.3±4 µmol troloxEQ/100 g fresh callus for DPPH and ABTS, respectively). These results are relevant as they show that UV light stress could be a physical elicitor for obtaining secondary metabolites with antioxidant activity in B. spinosa callus. http://rid.unrn.edu.ar/handle/20.500.12049/10287 en https://rafv2020.wixsite.com/santa-fe XXXIII Argentinean Meeting of Plant Physiology
institution Universidad Nacional de Río Negro
institution_str I-65
repository_str R-171
collection Repositorio Institucional Digital de la Universidad Nacional de Río Negro (UNRN)
language Inglés
orig_language_str_mv en
topic Biotecnología Agraria (General)
Elicitation
Bioactive compounds
ABTS
DPPH
Native flora
Biotecnología Agraria (General)
spellingShingle Biotecnología Agraria (General)
Elicitation
Bioactive compounds
ABTS
DPPH
Native flora
Biotecnología Agraria (General)
Dalzotto, Daniela Cecilia
Espíndola, Micaela
Huenelaf, Valeria Belén
Piñuel, María Lucrecia
Sharry, Sandra Elizabeth
Boeri, Patricia Alejandra
Secondary metabolites of Bougainvillea spinosa with antioxidant activity through callus grown in vitro under physical stress
topic_facet Biotecnología Agraria (General)
Elicitation
Bioactive compounds
ABTS
DPPH
Native flora
Biotecnología Agraria (General)
description Fil: Dalzotto, Daniela Cecilia. Universidad Nacional de Río Negro. Río Negro; Argentina
author Dalzotto, Daniela Cecilia
Espíndola, Micaela
Huenelaf, Valeria Belén
Piñuel, María Lucrecia
Sharry, Sandra Elizabeth
Boeri, Patricia Alejandra
author_facet Dalzotto, Daniela Cecilia
Espíndola, Micaela
Huenelaf, Valeria Belén
Piñuel, María Lucrecia
Sharry, Sandra Elizabeth
Boeri, Patricia Alejandra
author_sort Dalzotto, Daniela Cecilia
title Secondary metabolites of Bougainvillea spinosa with antioxidant activity through callus grown in vitro under physical stress
title_short Secondary metabolites of Bougainvillea spinosa with antioxidant activity through callus grown in vitro under physical stress
title_full Secondary metabolites of Bougainvillea spinosa with antioxidant activity through callus grown in vitro under physical stress
title_fullStr Secondary metabolites of Bougainvillea spinosa with antioxidant activity through callus grown in vitro under physical stress
title_full_unstemmed Secondary metabolites of Bougainvillea spinosa with antioxidant activity through callus grown in vitro under physical stress
title_sort secondary metabolites of bougainvillea spinosa with antioxidant activity through callus grown in vitro under physical stress
publishDate 2021
url http://rid.unrn.edu.ar/handle/20.500.12049/10287
work_keys_str_mv AT dalzottodanielacecilia secondarymetabolitesofbougainvilleaspinosawithantioxidantactivitythroughcallusgrowninvitrounderphysicalstress
AT espindolamicaela secondarymetabolitesofbougainvilleaspinosawithantioxidantactivitythroughcallusgrowninvitrounderphysicalstress
AT huenelafvaleriabelen secondarymetabolitesofbougainvilleaspinosawithantioxidantactivitythroughcallusgrowninvitrounderphysicalstress
AT pinuelmarialucrecia secondarymetabolitesofbougainvilleaspinosawithantioxidantactivitythroughcallusgrowninvitrounderphysicalstress
AT sharrysandraelizabeth secondarymetabolitesofbougainvilleaspinosawithantioxidantactivitythroughcallusgrowninvitrounderphysicalstress
AT boeripatriciaalejandra secondarymetabolitesofbougainvilleaspinosawithantioxidantactivitythroughcallusgrowninvitrounderphysicalstress
_version_ 1767996168077312000

Ejemplares similares