Pseudobutyrivibrio xylanivorans adhesion to epithelial cells
ARTÍCULO PUBLICADO EN REVISTA EXTERNA. The ruminal bacteria Pseudobutyrivibrio xylanivorans strain 2 (P. xylanivorans 2), that mediates the digestion of plant fiber, is considered an attractive candidate for probiotics. Adherence to the epithelium of the digestive tract of the host is one of the maj...
Guardado en:
Autores principales: | , , , , , , , |
---|---|
Formato: | Artículo Científico |
Lenguaje: | Inglés |
Publicado: |
Comité editorial Anaerobe Journal
2020
|
Materias: | |
Acceso en línea: | http://repositorio.umaza.edu.ar//handle/00261/1822 https://www.sciencedirect.com/science/article/abs/pii/S1075996419300010#! |
Aporte de: |
id |
I56-R16200261-1822 |
---|---|
record_format |
dspace |
institution |
Universidad Juan Agustín MAZA |
institution_str |
I-56 |
repository_str |
R-162 |
collection |
UMAZA Digital (Universidad MAZA - Mendoza) |
language |
Inglés |
orig_language_str_mv |
eng |
topic |
Rumen Anaerobic bacteria Probiotic Adhesion |
spellingShingle |
Rumen Anaerobic bacteria Probiotic Adhesion Grilli, Diego Javier Mansilla, María Eugenia Gimenez, María Cecilia Sohaefer, Noelia Ruiz, María Soledad Terebiznik, Mauricio Sosa, Miguel Arenas, Graciela Nora Pseudobutyrivibrio xylanivorans adhesion to epithelial cells |
description |
ARTÍCULO PUBLICADO EN REVISTA EXTERNA. The ruminal bacteria Pseudobutyrivibrio xylanivorans strain 2 (P. xylanivorans 2), that mediates the digestion of plant fiber, is considered an attractive candidate for probiotics. Adherence to the epithelium of the digestive tract of the host is one of the major requirements for probiotics. In this study, we
assessed the adhesion of P. xylanivorans 2 to SW480 cells and characterized this process utilizing multiple
microscopy approaches. Our results indicate that a multiplicity of infection of 200 CFU/cell allows the
highest bacteria to cell binding ratio, with a lower percentage of auto-agglutination events. The comparison
of the adherence capacity subjected heat-shock treatment (100 C, 1 min), which produces the
denaturalization of proteins at the bacterial surface, as opposed untreated P. xylanivorans, suggested that
this bacteria may attach to SW480 cells utilizing a proteinaceous structure. Confocal microscopy analyses
indicate that P. xylanivorans 2 attachment induces the formation of F-actin-enriched areas on the surface
of SW480 cells. Transmission electron microscopy (TEM) revealed the formation of a structure similar to
a pedestal in the area of the epithelial cell surface, where the bacterium rests. Finally, a casual finding of
TEM analysis of transverse and longitudinal thin-sections of P. xylanivorans 2, revealed irregular intracytoplasmic
structures compatibles with the so-called bacterial microcompartments. This is the first
ultrastructural description of bacterial microcompartments-like structures in the genus
Pseudobutyrivibrio. Sitio de la revista: https://www.sciencedirect.com/science/article/abs/pii/S1075996419300010 |
format |
Artículo Científico |
author |
Grilli, Diego Javier Mansilla, María Eugenia Gimenez, María Cecilia Sohaefer, Noelia Ruiz, María Soledad Terebiznik, Mauricio Sosa, Miguel Arenas, Graciela Nora |
author_facet |
Grilli, Diego Javier Mansilla, María Eugenia Gimenez, María Cecilia Sohaefer, Noelia Ruiz, María Soledad Terebiznik, Mauricio Sosa, Miguel Arenas, Graciela Nora |
author_sort |
Grilli, Diego Javier |
title |
Pseudobutyrivibrio xylanivorans adhesion to epithelial cells |
title_short |
Pseudobutyrivibrio xylanivorans adhesion to epithelial cells |
title_full |
Pseudobutyrivibrio xylanivorans adhesion to epithelial cells |
title_fullStr |
Pseudobutyrivibrio xylanivorans adhesion to epithelial cells |
title_full_unstemmed |
Pseudobutyrivibrio xylanivorans adhesion to epithelial cells |
title_sort |
pseudobutyrivibrio xylanivorans adhesion to epithelial cells |
publisher |
Comité editorial Anaerobe Journal |
publishDate |
2020 |
url |
http://repositorio.umaza.edu.ar//handle/00261/1822 https://www.sciencedirect.com/science/article/abs/pii/S1075996419300010#! |
work_keys_str_mv |
AT grillidiegojavier pseudobutyrivibrioxylanivoransadhesiontoepithelialcells AT mansillamariaeugenia pseudobutyrivibrioxylanivoransadhesiontoepithelialcells AT gimenezmariacecilia pseudobutyrivibrioxylanivoransadhesiontoepithelialcells AT sohaefernoelia pseudobutyrivibrioxylanivoransadhesiontoepithelialcells AT ruizmariasoledad pseudobutyrivibrioxylanivoransadhesiontoepithelialcells AT terebiznikmauricio pseudobutyrivibrioxylanivoransadhesiontoepithelialcells AT sosamiguel pseudobutyrivibrioxylanivoransadhesiontoepithelialcells AT arenasgracielanora pseudobutyrivibrioxylanivoransadhesiontoepithelialcells |
bdutipo_str |
Repositorios |
_version_ |
1764820541863100417 |