Evaluation of the genotoxic potential of cypermethrin, chlorpyrifos and their subsequent mixture, on cultured bovine lymphocytes

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Cypermethrin (CYP) and chlorpyrifos (CPF) are insecticides/parasiticides used in the production of fruits, vegetables and beef cattle. These substances or their metabolites are frequently reported as residues in food, whose consumption in a diet implies a genotoxic risk. The potential for chronic to...

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Autores principales: Ferré, Daniela Marisol, Ludueña, Héctor Ricardo, Romano, Raquel, Gorla, Nora Bibiana María
Formato: Institucional - Material de Difusión
Lenguaje:Inglés
Publicado: Comité editorial Chemosphere Journal 2020
Materias:
Cypermethrin
Chlorpyrifos
Micronucleus
Cell proliferation index
Parasiticides
CBMN Assay
Acceso en línea:http://repositorio.umaza.edu.ar//handle/00261/1823
https://www.sciencedirect.com/science/article/abs/pii/S0045653519325810#!
Aporte de:
UMAZA Digital (Universidad MAZA - Mendoza) de Universidad Juan Agustín MAZA
id I56-R162-00261-1823
record_format dspace
spelling I56-R162-00261-18232023-08-19T17:57:34Z Evaluation of the genotoxic potential of cypermethrin, chlorpyrifos and their subsequent mixture, on cultured bovine lymphocytes Ferré, Daniela Marisol Ludueña, Héctor Ricardo Romano, Raquel Gorla, Nora Bibiana María Cypermethrin Chlorpyrifos Micronucleus Cell proliferation index Parasiticides CBMN Assay Cypermethrin (CYP) and chlorpyrifos (CPF) are insecticides/parasiticides used in the production of fruits, vegetables and beef cattle. These substances or their metabolites are frequently reported as residues in food, whose consumption in a diet implies a genotoxic risk. The potential for chronic toxicity of CYP and CPF is unclear, and only a few genotoxicological evaluations based on their mixture have been performed. The aim of this study was to evaluate the genotoxic potential of CYP, CPF and CYP þ CPF in five concentrations, from 5.9 to 175 mg/mL, on bovine lymphocytes. By means of the cytokinesis-block micronucleus cytome assay, a decrease in the cell proliferation index was observed (r ¼ 0.89 p ¼ 0.04); and also an increase in the frequencies of binucleated cells (BN) with micronuclei (BNMn) (r ¼ 0.93, p ¼ 0.02) and BN with nuclear buds (BNBud) (r ¼ 0.778 p ¼ 0.04), depending on the concentrations of CPF. An increase in BNMn frequencies was observed as a function of CYP concentrations (r ¼ 0.89, p ¼ 0.04) and also of the CYP þ CPF mix (r ¼ 0.99, p ¼ 0.008). CYP caused greater genotoxic damage (BNMn) than CPF and the mixture on bovine lymphocytes. Cells with simultaneous presentation of micronuclei and nuclear buds were detected, as well as cells with irregular nuclei, something never previously reported, whose origin and significance should be investigated. The genotoxic effect of chlorpyrifos, cypermethrin and their mixture on bovine lymphocytes was observed. We recognized the value of the use of primary bovine cultures, animal species adjacent to man in the food chain, for genotoxicity studies. 2020-11-07T10:08:18Z 2020-11-07T10:08:18Z 2019-11-09 Institucional - Material de Difusión Ferré, D. M. Ludueña, H. R., Romano, R., y Gorla, N. B. M. (2019) Evaluation of the genotoxic potential of cypermethrin, chlorpyrifos and their subsequent mixture, on cultured bovine lymphocytes. Chemosphere, 243. https://doi.org/10.1016/j.chemosphere.2019.125341 0045-6535 http://repositorio.umaza.edu.ar//handle/00261/1823 https://www.sciencedirect.com/science/article/abs/pii/S0045653519325810#! eng image/png Comité editorial Chemosphere Journal 243;
institution Universidad Juan Agustín MAZA
institution_str I-56
repository_str R-162
collection UMAZA Digital (Universidad MAZA - Mendoza)
language Inglés
orig_language_str_mv eng
topic Cypermethrin
Chlorpyrifos
Micronucleus
Cell proliferation index
Parasiticides
CBMN Assay
spellingShingle Cypermethrin
Chlorpyrifos
Micronucleus
Cell proliferation index
Parasiticides
CBMN Assay
Ferré, Daniela Marisol
Ludueña, Héctor Ricardo
Romano, Raquel
Gorla, Nora Bibiana María
Evaluation of the genotoxic potential of cypermethrin, chlorpyrifos and their subsequent mixture, on cultured bovine lymphocytes
topic_facet Cypermethrin
Chlorpyrifos
Micronucleus
Cell proliferation index
Parasiticides
CBMN Assay
description Cypermethrin (CYP) and chlorpyrifos (CPF) are insecticides/parasiticides used in the production of fruits, vegetables and beef cattle. These substances or their metabolites are frequently reported as residues in food, whose consumption in a diet implies a genotoxic risk. The potential for chronic toxicity of CYP and CPF is unclear, and only a few genotoxicological evaluations based on their mixture have been performed. The aim of this study was to evaluate the genotoxic potential of CYP, CPF and CYP þ CPF in five concentrations, from 5.9 to 175 mg/mL, on bovine lymphocytes. By means of the cytokinesis-block micronucleus cytome assay, a decrease in the cell proliferation index was observed (r ¼ 0.89 p ¼ 0.04); and also an increase in the frequencies of binucleated cells (BN) with micronuclei (BNMn) (r ¼ 0.93, p ¼ 0.02) and BN with nuclear buds (BNBud) (r ¼ 0.778 p ¼ 0.04), depending on the concentrations of CPF. An increase in BNMn frequencies was observed as a function of CYP concentrations (r ¼ 0.89, p ¼ 0.04) and also of the CYP þ CPF mix (r ¼ 0.99, p ¼ 0.008). CYP caused greater genotoxic damage (BNMn) than CPF and the mixture on bovine lymphocytes. Cells with simultaneous presentation of micronuclei and nuclear buds were detected, as well as cells with irregular nuclei, something never previously reported, whose origin and significance should be investigated. The genotoxic effect of chlorpyrifos, cypermethrin and their mixture on bovine lymphocytes was observed. We recognized the value of the use of primary bovine cultures, animal species adjacent to man in the food chain, for genotoxicity studies.
format Institucional - Material de Difusión
author Ferré, Daniela Marisol
Ludueña, Héctor Ricardo
Romano, Raquel
Gorla, Nora Bibiana María
author_facet Ferré, Daniela Marisol
Ludueña, Héctor Ricardo
Romano, Raquel
Gorla, Nora Bibiana María
author_sort Ferré, Daniela Marisol
title Evaluation of the genotoxic potential of cypermethrin, chlorpyrifos and their subsequent mixture, on cultured bovine lymphocytes
title_short Evaluation of the genotoxic potential of cypermethrin, chlorpyrifos and their subsequent mixture, on cultured bovine lymphocytes
title_full Evaluation of the genotoxic potential of cypermethrin, chlorpyrifos and their subsequent mixture, on cultured bovine lymphocytes
title_fullStr Evaluation of the genotoxic potential of cypermethrin, chlorpyrifos and their subsequent mixture, on cultured bovine lymphocytes
title_full_unstemmed Evaluation of the genotoxic potential of cypermethrin, chlorpyrifos and their subsequent mixture, on cultured bovine lymphocytes
title_sort evaluation of the genotoxic potential of cypermethrin, chlorpyrifos and their subsequent mixture, on cultured bovine lymphocytes
publisher Comité editorial Chemosphere Journal
publishDate 2020
url http://repositorio.umaza.edu.ar//handle/00261/1823
https://www.sciencedirect.com/science/article/abs/pii/S0045653519325810#!
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AT romanoraquel evaluationofthegenotoxicpotentialofcypermethrinchlorpyrifosandtheirsubsequentmixtureonculturedbovinelymphocytes
AT gorlanorabibianamaria evaluationofthegenotoxicpotentialofcypermethrinchlorpyrifosandtheirsubsequentmixtureonculturedbovinelymphocytes
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