Analysis of the rumen bacterial diversity of goats during shift from forage to concentrate diet.

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High-grain feeding used in the animal production is known to affect the host rumen bacterial community, but our understanding of consequent changes in goats is limited. This study was therefore aimed to evaluate bacterial population dynamics during 20 days adaptation of 4 ruminally cannulated goats...

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Autores principales: Grilli, Diego Javier, Fliegerová, Kateřina, Kopečný, Jan, Paez Lama, Sebastián, Egea, Vanina, Sohaefer, Noelia, Pereyra, Celia, Ruiz, María Soledad, Sosa, Miguel Angel, Arenas, Graciela Nora, Mrázek, Jakub
Formato: Insitucional - Material de Difusión
Lenguaje:Inglés
Publicado: Comité editor Journal Anaerobe 2020
Materias:
Creole Goats
Rumens
Bacterias
PCR-DGGE
QPCR
Acceso en línea:http://repositorio.umaza.edu.ar//handle/00261/1784
https://www.sciencedirect.com/science/article/abs/pii/S1075996416300865
Aporte de:
UMAZA Digital (Universidad MAZA - Mendoza) de Universidad Juan Agustín MAZA
id I56-R162-00261-1784
record_format dspace
spelling I56-R162-00261-17842023-09-14T02:08:20Z Analysis of the rumen bacterial diversity of goats during shift from forage to concentrate diet. Grilli, Diego Javier Fliegerová, Kateřina Kopečný, Jan Paez Lama, Sebastián Egea, Vanina Sohaefer, Noelia Pereyra, Celia Ruiz, María Soledad Sosa, Miguel Angel Arenas, Graciela Nora Mrázek, Jakub Creole Goats Rumens Bacterias PCR-DGGE QPCR High-grain feeding used in the animal production is known to affect the host rumen bacterial community, but our understanding of consequent changes in goats is limited. This study was therefore aimed to evaluate bacterial population dynamics during 20 days adaptation of 4 ruminally cannulated goats to the high-grain diet (grain: hay e ratio of 40:60). The dietary transition of goats from the forage to the high-grain-diet resulted in the significant decrease of rumen fluid pH, which was however still higher than value established for acute or subacute ruminal acidosis was not diagnosed in studied animals. DGGE analysis demonstrated distinct ruminal microbial populations in hay-fed and grain-fed animals, but the substantial animal-to-animal variation were detected. Quantitative PCR showed for grain-fed animals significantly higher number of bacteria belonging to Clostridium leptum group at 10 days after the incorporation of corn into the diet and significantly lower concentration of bacteria belonging to Actinobacteria phylum at the day 20 after dietary change. Taxonomic distribution analysed by NGS at day 20 revealed the similar prevalence of the phyla Firmicutes and Bacteroidetes in all goats, significantly higher presence of the unclassified genus of groups of Bacteroidales and Ruminococcaceae in grain-fed animals and significantly higher presence the genus Prevotella and Butyrivibrio in the forage-fed animals. The three different culture-independent methods used in this study show that high proportion of concentrate in goat diet does not induce any serious disturbance of their rumen ecosystem and indicate the good adaptive response of caprine ruminal bacteria to incorporation of corn into the diet. 2020-10-16T19:27:12Z 2020-10-16T19:27:12Z 2016-07-12 Insitucional - Material de Difusión Grilli, D. J., Fliegerová, K., Kopecný J., Paez Lama, S., Egea, V., Sohaefer, N., Pereyra C., Ruiz, M.S., sosa, M. A., Arenas G. N., y Mrázek, J.(2016) Analysis of the rumen bacterial diversity of goats during shift from forage to concentrate diet. Anaerobe Journal, 42, 17-26. https://doi.org/10.1016/j.anaerobe.2016.07.002 1075-9964 http://repositorio.umaza.edu.ar//handle/00261/1784 https://www.sciencedirect.com/science/article/abs/pii/S1075996416300865 eng image/png Comité editor Journal Anaerobe 42;
institution Universidad Juan Agustín MAZA
institution_str I-56
repository_str R-162
collection UMAZA Digital (Universidad MAZA - Mendoza)
language Inglés
orig_language_str_mv eng
topic Creole Goats
Rumens
Bacterias
PCR-DGGE
QPCR
spellingShingle Creole Goats
Rumens
Bacterias
PCR-DGGE
QPCR
Grilli, Diego Javier
Fliegerová, Kateřina
Kopečný, Jan
Paez Lama, Sebastián
Egea, Vanina
Sohaefer, Noelia
Pereyra, Celia
Ruiz, María Soledad
Sosa, Miguel Angel
Arenas, Graciela Nora
Mrázek, Jakub
Analysis of the rumen bacterial diversity of goats during shift from forage to concentrate diet.
topic_facet Creole Goats
Rumens
Bacterias
PCR-DGGE
QPCR
description High-grain feeding used in the animal production is known to affect the host rumen bacterial community, but our understanding of consequent changes in goats is limited. This study was therefore aimed to evaluate bacterial population dynamics during 20 days adaptation of 4 ruminally cannulated goats to the high-grain diet (grain: hay e ratio of 40:60). The dietary transition of goats from the forage to the high-grain-diet resulted in the significant decrease of rumen fluid pH, which was however still higher than value established for acute or subacute ruminal acidosis was not diagnosed in studied animals. DGGE analysis demonstrated distinct ruminal microbial populations in hay-fed and grain-fed animals, but the substantial animal-to-animal variation were detected. Quantitative PCR showed for grain-fed animals significantly higher number of bacteria belonging to Clostridium leptum group at 10 days after the incorporation of corn into the diet and significantly lower concentration of bacteria belonging to Actinobacteria phylum at the day 20 after dietary change. Taxonomic distribution analysed by NGS at day 20 revealed the similar prevalence of the phyla Firmicutes and Bacteroidetes in all goats, significantly higher presence of the unclassified genus of groups of Bacteroidales and Ruminococcaceae in grain-fed animals and significantly higher presence the genus Prevotella and Butyrivibrio in the forage-fed animals. The three different culture-independent methods used in this study show that high proportion of concentrate in goat diet does not induce any serious disturbance of their rumen ecosystem and indicate the good adaptive response of caprine ruminal bacteria to incorporation of corn into the diet.
format Insitucional - Material de Difusión
author Grilli, Diego Javier
Fliegerová, Kateřina
Kopečný, Jan
Paez Lama, Sebastián
Egea, Vanina
Sohaefer, Noelia
Pereyra, Celia
Ruiz, María Soledad
Sosa, Miguel Angel
Arenas, Graciela Nora
Mrázek, Jakub
author_facet Grilli, Diego Javier
Fliegerová, Kateřina
Kopečný, Jan
Paez Lama, Sebastián
Egea, Vanina
Sohaefer, Noelia
Pereyra, Celia
Ruiz, María Soledad
Sosa, Miguel Angel
Arenas, Graciela Nora
Mrázek, Jakub
author_sort Grilli, Diego Javier
title Analysis of the rumen bacterial diversity of goats during shift from forage to concentrate diet.
title_short Analysis of the rumen bacterial diversity of goats during shift from forage to concentrate diet.
title_full Analysis of the rumen bacterial diversity of goats during shift from forage to concentrate diet.
title_fullStr Analysis of the rumen bacterial diversity of goats during shift from forage to concentrate diet.
title_full_unstemmed Analysis of the rumen bacterial diversity of goats during shift from forage to concentrate diet.
title_sort analysis of the rumen bacterial diversity of goats during shift from forage to concentrate diet.
publisher Comité editor Journal Anaerobe
publishDate 2020
url http://repositorio.umaza.edu.ar//handle/00261/1784
https://www.sciencedirect.com/science/article/abs/pii/S1075996416300865
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