Ruminal bacterial community changes during adaptation of goats to fresh alfalfa forage.

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Using culture-independent molecular approaches, we studied the bacterial population changes in the rumen of goats abruptly converted from alfalfa hay to fresh alfalfa diet. Administration of fresh forage with significantly increased soluble nitrogen and soluble protein nitrogen resulted in frothy bl...

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Detalles Bibliográficos
Autores principales: Grilli, Diego Javier, Mrázek, Jakub, Fliegerová, Kateřina, Kopečný, Jan, Paez Lama, Sebastian, Cerón Cucchi, María Esperanza, Sosa, Miguel Angel, Arenas, Graciela Nora
Formato: Institucional - Material de Difusión
Lenguaje:Inglés
Publicado: Editorial Livestock Science 2020
Materias:
Bacterias
Creole Goats
QPCR
PCR-DGGE
Rumens
Acceso en línea:http://repositorio.umaza.edu.ar//handle/00261/1747
https://www.sciencedirect.com/science/article/abs/pii/S187114131630172X#!
Aporte de:
UMAZA Digital (Universidad MAZA - Mendoza) de Universidad Juan Agustín MAZA
id I56-R162-00261-1747
record_format dspace
spelling I56-R162-00261-17472023-09-14T02:10:29Z Ruminal bacterial community changes during adaptation of goats to fresh alfalfa forage. Grilli, Diego Javier Mrázek, Jakub Fliegerová, Kateřina Kopečný, Jan Paez Lama, Sebastian Cerón Cucchi, María Esperanza Sosa, Miguel Angel Arenas, Graciela Nora Bacterias Creole Goats QPCR PCR-DGGE Rumens Using culture-independent molecular approaches, we studied the bacterial population changes in the rumen of goats abruptly converted from alfalfa hay to fresh alfalfa diet. Administration of fresh forage with significantly increased soluble nitrogen and soluble protein nitrogen resulted in frothy bloat. Changes of the bacterial composition of rumen were monitored using DGGE analysis of 16S rDNA gene amplicons and quantitative PCR method. As the diet changed, the bacterial population of Bacteroidetes and γ-Proteobacteria decreased, even if animals have not shown signs of frothy bloat. The most severely bloated animals showed an increase of Bacteroidetes phylum. Lactobacillus/Streptococcus group belonging to Firmicutes phylum decreased in response to transferring the animals from hay to a fresh forage-based diet, and did not achieve the values observed at the beginning of the experiment. In summary, changes in the diet and subsequent frothy bloat occurrence produce long-lasting changes in the structure of the microbial community and may be associated with a specific bacterial population belonging to the Bacteroidetes phylum. 2020-10-06T13:39:04Z 2020-10-06T13:39:04Z 2016-08-02 Institucional - Material de Difusión Grili, D. J., Mrázek, J., Fliegerová, K., Kopečný, J., Paez Lama, S., Cerón Cucchi, M. E., Sosa, M. A., y Arenas, G. N. (2016) Ruminal bacterial community changes during adaptation of goats to fresh alfalfa forage. Livestock Science, 191, 191-195. https://doi.org/10.1016/j.livsci.2016.08.001 1871-1413 http://repositorio.umaza.edu.ar//handle/00261/1747 https://www.sciencedirect.com/science/article/abs/pii/S187114131630172X#! eng image/png Editorial Livestock Science 191;
institution Universidad Juan Agustín MAZA
institution_str I-56
repository_str R-162
collection UMAZA Digital (Universidad MAZA - Mendoza)
language Inglés
orig_language_str_mv eng
topic Bacterias
Creole Goats
QPCR
PCR-DGGE
Rumens
spellingShingle Bacterias
Creole Goats
QPCR
PCR-DGGE
Rumens
Grilli, Diego Javier
Mrázek, Jakub
Fliegerová, Kateřina
Kopečný, Jan
Paez Lama, Sebastian
Cerón Cucchi, María Esperanza
Sosa, Miguel Angel
Arenas, Graciela Nora
Ruminal bacterial community changes during adaptation of goats to fresh alfalfa forage.
topic_facet Bacterias
Creole Goats
QPCR
PCR-DGGE
Rumens
description Using culture-independent molecular approaches, we studied the bacterial population changes in the rumen of goats abruptly converted from alfalfa hay to fresh alfalfa diet. Administration of fresh forage with significantly increased soluble nitrogen and soluble protein nitrogen resulted in frothy bloat. Changes of the bacterial composition of rumen were monitored using DGGE analysis of 16S rDNA gene amplicons and quantitative PCR method. As the diet changed, the bacterial population of Bacteroidetes and γ-Proteobacteria decreased, even if animals have not shown signs of frothy bloat. The most severely bloated animals showed an increase of Bacteroidetes phylum. Lactobacillus/Streptococcus group belonging to Firmicutes phylum decreased in response to transferring the animals from hay to a fresh forage-based diet, and did not achieve the values observed at the beginning of the experiment. In summary, changes in the diet and subsequent frothy bloat occurrence produce long-lasting changes in the structure of the microbial community and may be associated with a specific bacterial population belonging to the Bacteroidetes phylum.
format Institucional - Material de Difusión
author Grilli, Diego Javier
Mrázek, Jakub
Fliegerová, Kateřina
Kopečný, Jan
Paez Lama, Sebastian
Cerón Cucchi, María Esperanza
Sosa, Miguel Angel
Arenas, Graciela Nora
author_facet Grilli, Diego Javier
Mrázek, Jakub
Fliegerová, Kateřina
Kopečný, Jan
Paez Lama, Sebastian
Cerón Cucchi, María Esperanza
Sosa, Miguel Angel
Arenas, Graciela Nora
author_sort Grilli, Diego Javier
title Ruminal bacterial community changes during adaptation of goats to fresh alfalfa forage.
title_short Ruminal bacterial community changes during adaptation of goats to fresh alfalfa forage.
title_full Ruminal bacterial community changes during adaptation of goats to fresh alfalfa forage.
title_fullStr Ruminal bacterial community changes during adaptation of goats to fresh alfalfa forage.
title_full_unstemmed Ruminal bacterial community changes during adaptation of goats to fresh alfalfa forage.
title_sort ruminal bacterial community changes during adaptation of goats to fresh alfalfa forage.
publisher Editorial Livestock Science
publishDate 2020
url http://repositorio.umaza.edu.ar//handle/00261/1747
https://www.sciencedirect.com/science/article/abs/pii/S187114131630172X#!
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