Effect of monospecific antibodies against baltergin in myotoxicity induced by Bothrops alternatus venom from northeast of Argentina. Role of metalloproteinases in muscle damage
Myotoxicity, one of the most relevant local manifestations in envenomation by Bothrops genus, may result from a direct action of myotoxins or be due to an indirect vascular degeneration and ischemia. Baltergin, a snake venom metalloproteinase (SVMP), isolated from Bothrops alternatus venom has be...
Guardado en:
| Autores principales: | , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | Español |
| Publicado: |
Elsevier Ltd.
2025
|
| Materias: | |
| Acceso en línea: | http://repositorio.unne.edu.ar/handle/123456789/58049 |
| Aporte de: |
| Sumario: | Myotoxicity, one of the most relevant local manifestations in envenomation by Bothrops
genus, may result from a direct action of myotoxins or be due to an indirect vascular
degeneration and ischemia. Baltergin, a snake venom metalloproteinase (SVMP), isolated
from Bothrops alternatus venom has been used to obtain monospecific IgG, in order to
determine the relative role of toxin in myotoxicity induced by whole venom. Bothrops
diporus venom, another medical relevant genus of the northeastern region of Argentina,
was also studied. Anti-baltergin IgG was able to neutralize completely the hemorrhagic
activity of B. alternatus venom at an antibodies:venom ratio of 30:1 (w:w). However, mice
injected with B. diporus venom showed a small spot remaining even at the highest ratio of
IgG:venom assayed (50:1; w:w). Specific antibodies were efficient to neutralize the
myotoxicity of B. alternatus venom at ratio 30:1 (w:w) but did not neutralize the same
effects in B. diporus venom. Anti-baltergin polyclonal antibodies were useful tools for
revealing the central role of SVMPs in the development of myotoxicity of B. alternatus
venom, as well as, helping to suggest indirectly presence of potent myotoxic phospholi-
pases A2 (PLA2s) in B. diporus venom. |
|---|