Optimization of an in vitro regeneration system for indica rice varieties
In order to optimize an in vitro regeneration system for colombian rice varieties, the amount of plant regulators used for each step of the process was modified, together with other factors such as seed position and time of exposition to plant regulators. Modification of 2,4-D concentration during...
Guardado en:
| Autores principales: | , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Universidad Nacional de Colombia. Instituto de Biotecnología
2023
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| Materias: | |
| Acceso en línea: | http://repositorio.unne.edu.ar/handle/123456789/51239 |
| Aporte de: |
| Sumario: | In order to optimize an in vitro regeneration system for colombian rice varieties, the amount of plant regulators used for
each step of the process was modified, together with other factors such as seed position and time of exposition to plant
regulators. Modification of 2,4-D concentration during callus induction and coculture has a negative effect on regeneration
potential. However, it is possible to increase the amount of produced calli by modifying the position in which the seed is
placed on the culture medium. Moreover, a longer callus induction time decreased the regeneration percentage. During
the regeneration step, for FEDEARROZ 2000 variety, a combination of 1,0 mg/l g/l mg/l of naftalenacetic acid + 4,0 mg/l
g/l mg/l of kinetin increases the regeneration percentage, without increasing the amount of abnormal shoots and without
decreasing their size. For FEDEARROZ 50 variety, the best combination of plant regulators is 0,5 mg/l g/l mg/l of naftalena-
cetic acid + 2,0 mg/l g/l mg/l of kinetin. |
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