Mice plasma fibrinogen consumption by thrombin-like enzyme present in rattlesnake venom from the north-east region of Argentina
Due to variability of venom components from the same species of snakes that inhabit different regions, particular properties of the venom of Crotalus durissus terrificus that inhabits the North-East of Ar- gentina were studied. Gyroxin, a thrombin-like enzyme, was isolated from this venom by gel...
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| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Fundación Revista Medicina
2022
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| Materias: | |
| Acceso en línea: | http://repositorio.unne.edu.ar/handle/123456789/48604 |
| Aporte de: |
| Sumario: | Due to variability of venom components from the same species of snakes that inhabit different regions,
particular properties of the venom of Crotalus durissus terrificus that inhabits the North-East of Ar-
gentina were studied. Gyroxin, a thrombin-like enzyme, was isolated from this venom by gel filtration and affinity
chromatography, it was found to be homogeneous according to SDS-PAGE, with a molecular weight of 33 kDa.
”Gyroxin syndrome” in mice was tested and it showed changes in the animal behavior, confirming that the isolated
thrombin-like enzyme is gyroxin. Effects of this enzyme and the crude venom on mice plasmatic fibrinogen levels
were determined. The mice plasma fibrinogen decreased rapidly until incoagulability during the first hour after
thrombin-Iike enzyme injection, then reaching its normal level 10 hours after injection; whereas crude venom
resulted in a 60% decrease of the mice plasma fibrinogen, reaching its normal level after the same period of
time. After 1 hour of gyroxin inoculation, intravascular coagulation was observed in histological cuttings of lung,
cardiac muscle and liver. The isolated enzyme showed strong hydrolyzing activity on fibrinogen and fibrin in
vitro, whereas the crude venom exhibited weak hydrolyzing activity on both substrates. It is probable that this
very low activity is due to the low percentage of the enzyme in the crude venom. Decreasing of plasmatic
fibrinogen levels may be due to either the coagulant or hydrolyzing actions of the enzyme. |
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