Frequency and associated factors of Ehrlichia canis and Anaplasma spp. in dogs from a peri-urban community of Yucatan, Mexico

Ehrlichia and Anaplasma are bacterial genera (family Anaplasmataceae, order Rickettsiales) that include tick-borne species responsible for emerging and reemerging diseases of importance to both animal and public health. Dogs living in regions with abundant tick vectors may act as hosts for Ehrlichia...

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Autores principales: Osorio-Primo, T., Lugo-Caballero, C., Noh-Pech, H., Suárez-Galaz, A.R., Sánchez-Montes, S., Arroyo-Ramírez, A., Rodríguez-Vivas, R.I., Flota-Burgos, G., Ochoa-Valencia, J.L, Bolio-González, M, Torres-Castro, M.
Formato: Artículo revista
Lenguaje:Español
Publicado: Universidad Nacional del Nordeste 2026
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Acceso en línea:https://revistas.unne.edu.ar/index.php/vet/article/view/9291
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Sumario:Ehrlichia and Anaplasma are bacterial genera (family Anaplasmataceae, order Rickettsiales) that include tick-borne species responsible for emerging and reemerging diseases of importance to both animal and public health. Dogs living in regions with abundant tick vectors may act as hosts for Ehrlichia and Anaplasma species. The objective of this study was to determine the DNA detection frequency of Ehrlichia and Anaplasma, characterize the circulating species, and identify factors associated with their occurrence in dogs from a peri-urban community in Yucatan, Mexico. A total of 67 dogs (n=67) from Ucú, Yucatan, were included in the study. Blood samples were collected, and demographic and environmental characteristics were recorded. Genomic DNA was extracted and used for the detection of Ehrlichia and Anaplasma by endpoint PCR. In addition, bioinformatic tools and phylogenetic analyses were used to characterize the detected species. Chi-square or Fisher's exact test, as appropriate, was performed to identify significant associations (p<0.05) between positive cases and the response variables. Overall, 62.6% (42/67) of dogs tested positive for Anaplasmataceae, 61.1% (41/67) for Ehrlichia, and 17.9% (12/67) for Anaplasma. Sequence and phylogenetic analyses revealed homologies with E. canis, A. platys, and A. phagocytophilum. Significant associations were found between Ehrlichia frequency and the variables “non-ideal” body condition (p=0.042), zone of the dwelling "interior" (p=0.009), and zone of the dwelling "exterior" (p=0.002), while the zone of the dwelling "central" (p=0.006) was associated with Anaplasma frequency. The circulation of these bacteria was confirmed in the studied dogs. These findings suggest that the distributions of E. canis and Anaplasma spp. is not homogeneous within the study area. Future studies should evaluate the eco-epidemiological factors influencing the local distribution of these pathogens.