From preliminary data to robust design: sample estimation for Anaplasma phagocytophilum and other prevalence studies

Accurate sample size estimation is essential in prevalence studies to ensure statistically valid conclusions and efficient resource allocation. This technical note presents an approach for estimating sample size in prevalence studies of Anaplasma phagocytophilum in equines. A preliminary PCR-based s...

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Autores principales: Araúz, Anayansi, Santana Bolívar, Ángel, Morales Allard, Joel Alexis, De Sedas Muñoz, Abraham, Añino Ramos, Yostin Jesús, Agrazal, Karla Jeanneette, Romero, Emilio
Formato: Artículo revista
Lenguaje:Español
Publicado: Universidad Nacional del Nordeste 2026
Materias:
Prevalence studies
Precision
Sample size
ScalaR
estudios de prevalencia
precisión
tamaño de muestra
Acceso en línea:https://revistas.unne.edu.ar/index.php/vet/article/view/9106
Aporte de:
Revistas UNNE - Universidad Nacional del Noroeste (UNNE) de Universidad Nacional del Nordeste
id I48-R154-article-9106
record_format ojs
spelling I48-R154-article-91062026-02-04T15:47:47Z From preliminary data to robust design: sample estimation for Anaplasma phagocytophilum and other prevalence studies De los datos preliminares al diseño robusto: estimación de muestras para estudios de Anaplasma phagocytophilum y otros estudios de prevalencia Araúz, Anayansi Santana Bolívar, Ángel Morales Allard, Joel Alexis De Sedas Muñoz, Abraham Añino Ramos, Yostin Jesús Agrazal, Karla Jeanneette Romero, Emilio Prevalence studies Precision Sample size ScalaR estudios de prevalencia precisión tamaño de muestra ScalaR Accurate sample size estimation is essential in prevalence studies to ensure statistically valid conclusions and efficient resource allocation. This technical note presents an approach for estimating sample size in prevalence studies of Anaplasma phagocytophilum in equines. A preliminary PCR-based survey in Panama detected one positive case among 36 animals (2.78%). Based on these results, expected prevalences of 2%, 3%, 8%, and 9% were used to model future sample sizes using the ScalaR function in R, incorporating absolute precisions (± 0.75%, ± 1.5%, ± 2%) and a 10% adjustment for potential data loss. The estimated sample sizes ranged from 233 (2% prevalence, ± 1.9%) to 6,216 animals (9% prevalence, ± 0.75%). These findings illustrate how minor variations in assumed prevalence or desired precision can substantially influence the sample size required for reliable epidemiological surveillance. La estimación precisa del tamaño de la muestra es esencial en los estudios de prevalencia para garantizar conclusiones estadísticamente válidas y un uso eficiente de los recursos. Esta nota técnica presenta un enfoque de estimación del tamaño de la muestra para estudios de prevalencia de Anaplasma phagocytophilum en equinos. Un estudio preliminar basado en PCR en Panamá arrojó un caso positivo entre 36 animales (2,78%). Con base en esto, se utilizaron prevalencias esperadas del 2%, 3%, 8% y 9% para modelar futuros tamaños de muestra mediante la función ScalaR en R, incorporando precisiones absolutas (± 0,75%, ± 1,5%, ± 2%) y un ajuste por pérdida de datos del 10%. Los tamaños de muestra estimados oscilaron entre 233 (prevalencia del 2%, ± 1,9%) y 6.216 animales (prevalencia del 9%, ± 0,75%). Estas estimaciones destacan cómo pequeños cambios en los supuestos de precisión y prevalencia pueden afectar considerablemente el tamaño de muestra requerido para estudios de vigilancia fiables. Universidad Nacional del Nordeste 2026-02-04 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unne.edu.ar/index.php/vet/article/view/9106 10.30972/vet.3719106 Revista Veterinaria; Vol. 37 (2026); 1-4 Revista Veterinaria; Vol. 37 (2026); 1-4 1669-6840 1668-4834 spa https://revistas.unne.edu.ar/index.php/vet/article/view/9106/8822 Derechos de autor 2026 Anayansi Araúz, Ángel Santana Bolívar, Joel Alexis Morales Allard, Abraham De Sedas Muñoz, Yostin Jesús Añino Ramos, Karla Jeanneette Agrazal, Emilio Romero https://creativecommons.org/licenses/by-nc/4.0
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-154
container_title_str Revistas UNNE - Universidad Nacional del Noroeste (UNNE)
language Español
format Artículo revista
topic Prevalence studies
Precision
Sample size
ScalaR
estudios de prevalencia
precisión
tamaño de muestra
ScalaR
spellingShingle Prevalence studies
Precision
Sample size
ScalaR
estudios de prevalencia
precisión
tamaño de muestra
ScalaR
Araúz, Anayansi
Santana Bolívar, Ángel
Morales Allard, Joel Alexis
De Sedas Muñoz, Abraham
Añino Ramos, Yostin Jesús
Agrazal, Karla Jeanneette
Romero, Emilio
From preliminary data to robust design: sample estimation for Anaplasma phagocytophilum and other prevalence studies
topic_facet Prevalence studies
Precision
Sample size
ScalaR
estudios de prevalencia
precisión
tamaño de muestra
ScalaR
author Araúz, Anayansi
Santana Bolívar, Ángel
Morales Allard, Joel Alexis
De Sedas Muñoz, Abraham
Añino Ramos, Yostin Jesús
Agrazal, Karla Jeanneette
Romero, Emilio
author_facet Araúz, Anayansi
Santana Bolívar, Ángel
Morales Allard, Joel Alexis
De Sedas Muñoz, Abraham
Añino Ramos, Yostin Jesús
Agrazal, Karla Jeanneette
Romero, Emilio
author_sort Araúz, Anayansi
title From preliminary data to robust design: sample estimation for Anaplasma phagocytophilum and other prevalence studies
title_short From preliminary data to robust design: sample estimation for Anaplasma phagocytophilum and other prevalence studies
title_full From preliminary data to robust design: sample estimation for Anaplasma phagocytophilum and other prevalence studies
title_fullStr From preliminary data to robust design: sample estimation for Anaplasma phagocytophilum and other prevalence studies
title_full_unstemmed From preliminary data to robust design: sample estimation for Anaplasma phagocytophilum and other prevalence studies
title_sort from preliminary data to robust design: sample estimation for anaplasma phagocytophilum and other prevalence studies
description Accurate sample size estimation is essential in prevalence studies to ensure statistically valid conclusions and efficient resource allocation. This technical note presents an approach for estimating sample size in prevalence studies of Anaplasma phagocytophilum in equines. A preliminary PCR-based survey in Panama detected one positive case among 36 animals (2.78%). Based on these results, expected prevalences of 2%, 3%, 8%, and 9% were used to model future sample sizes using the ScalaR function in R, incorporating absolute precisions (± 0.75%, ± 1.5%, ± 2%) and a 10% adjustment for potential data loss. The estimated sample sizes ranged from 233 (2% prevalence, ± 1.9%) to 6,216 animals (9% prevalence, ± 0.75%). These findings illustrate how minor variations in assumed prevalence or desired precision can substantially influence the sample size required for reliable epidemiological surveillance.
publisher Universidad Nacional del Nordeste
publishDate 2026
url https://revistas.unne.edu.ar/index.php/vet/article/view/9106
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first_indexed 2026-02-17T05:00:48Z
last_indexed 2026-02-17T05:00:48Z
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