Structural analysis of the effect of melatonin on the submandibular gland of rats treated with cyclophosphamide
Cyclophosphamide is a cytostatic agent used in conditioning regimens for bone marrow transplantation and in some solid tumors. It has the capacity to induce alterations in several organ systems, particularly the stomatognathic system. Previous studies by our team demonstrated an oxidative and functi...
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| Autores principales: | , , , , , |
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| Formato: | Artículo revista |
| Lenguaje: | Español |
| Publicado: |
Facultad de Odontología de la Universidad Nacional del Nordeste (FOUNNE)
2025
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| Materias: | |
| Acceso en línea: | https://revistas.unne.edu.ar/index.php/rfo/article/view/9030 |
| Aporte de: |
| Sumario: | Cyclophosphamide is a cytostatic agent used in conditioning regimens for bone marrow transplantation and in some solid tumors. It has the capacity to induce alterations in several organ systems, particularly the stomatognathic system. Previous studies by our team demonstrated an oxidative and functional effect of this drug, assessed through increased superoxide dismutase, uric acid, and glycogen levels in rat submandibular gland. On the other hand, melatonin is known for its multipurpose action due to its capacity to attenuate structural and functional alterations in several organs with high functional activity and mitotic rates. In the present study, we evaluated histological changes in ducts and acini of the submandibular gland of rats treated with cyclophosphamide, as well as the protective effect of exogenously administered melatonin. Three-month-old adult male Wistar rats with body weight ranging from 300 to 350 grams, were used and divided into five experimental groups: 1) C: Control, 2) Control and Ethanol, 3) Cyclophosphamide, 4) Cyclophosphamide and Melatonin, and 5) Melatonin. Histological sections of submandibular glands were prepared and stained with Hematoxylin/Eosin, where possible structural changes in ducts and acini were analyzed. Glands from animals treated with cyclophosphamide showed acinar cells with macrokaryosis and hyperchromasia, binucleation with irregular contours, prominent nucleoli, and perinuclear chromatin clumps compared to control groups. It is inferred that melatonin administration at the tested dose could attenuate the structural alterations of the submandibular gland treated with this chemotherapeutic agent. The reversal of such changes would explain the reduction of antioxidant and functional effects at the glandular level described in our laboratory. |
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