Identification and selection of tetraploid citrus rootstocks by flow cytometry and leaf colorimetry análisis

Argentina is a major citrus-producing country on the global scale, with cultivation primarily concentrated in the Argentina Northwest (NOA) and Northeast (NEA) regions. Modern citrus production relies on the use of rootstocks as a critical tool to improve tree uniformity, yield, and resilience under...

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Autores principales: Garavello, Miguel F., Recalde, José M., Bello, Fernando, Galdeano, Florencia, Alayón Luaces, Paula
Formato: Artículo revista
Lenguaje:Español
Publicado: Instituto de Botánica del Nordeste 2025
Materias:
Citrus
contenido de ADN
croma
poliploides
Chroma
dna content
polyploids
Acceso en línea:https://revistas.unne.edu.ar/index.php/bon/article/view/8556
Aporte de:
Revistas UNNE - Universidad Nacional del Noroeste (UNNE) de Universidad Nacional del Nordeste
id I48-R154-article-8556
record_format ojs
spelling I48-R154-article-85562025-08-14T23:20:42Z Identification and selection of tetraploid citrus rootstocks by flow cytometry and leaf colorimetry análisis Identificación y selección de portainjertos tetraploides de cítricos por medio de citometría de flujo y análisis de colorimetría de hojas Garavello, Miguel F. Recalde, José M. Bello, Fernando Galdeano, Florencia Alayón Luaces, Paula Citrus contenido de ADN croma poliploides Chroma Citrus dna content polyploids Argentina is a major citrus-producing country on the global scale, with cultivation primarily concentrated in the Argentina Northwest (NOA) and Northeast (NEA) regions. Modern citrus production relies on the use of rootstocks as a critical tool to improve tree uniformity, yield, and resilience under adverse biotic and abiotic conditions. This study focused on identifying and selecting spontaneous tetraploid individuals among five rootstock genotypes ‘Trifolio Concordia’ [Poncirus trifoliata (L.) Raf.], ‘Rubidoux’ (P. trifoliata), ‘X639’ [Citrus reshni Hort. ex Tanaka × P. trifoliata], ‘Citrange Troyer’ [C. sinensis (L.) Osbeck × P. trifoliata] y ‘Citrumelo Swingle’ (Citrus paradisi Macfad. × P. trifoliata). Through preliminary morphological screening and subsequent flow cytometry analysis, thirteen tetraploid individuals were confirmed among thirty-seven candidates. Tetraploid frequencies ranged from 66.7% in ‘Rubidoux’ to 12.5% in ‘Citrumelo Swingle’. Flow cytometry demonstrated high efficacy for ploidy determination, while leaf colorimetry effectively discriminated between diploid and tetraploid plants, with the most pronounced differences observed in ‘X639’ (L* = 5.1; a* = –3.5; b* = 7.6). These findings provide a solid basis for future research and applications in the citrus industry. La citricultura en Argentina ocupa un lugar destacado a nivel mundial, con una producción concentrada principalmente en las regiones del noroeste argentino (NOA) y noreste argentino (NEA). El cultivo moderno de cítricos requiere el uso de portainjertos como herramienta esencial para mejorar la uniformidad, la productividad y la resistencia frente a condiciones adversas. Este estudio se centró en la identificación y selección de portainjertos tetraploides espontáneos a partir de cinco genotipos: ‘Trifolio Concordia’ [Poncirus trifoliata (L.) Raf.]; ‘Rubidoux’ (P. trifoliata); ‘X639’ (Citrus reshni Hort. ex Tanaka × P. trifoliata); ‘Citrange Troyer’ [C. sinensis (L.) Osbeck × P. trifoliata]; y ‘Citrumelo Swingle’ (Citrus paradisi Macfad. × P. trifoliata). A partir de la evaluación morfológica y la citometría de flujo, se confirmaron 13 plantas tetraploides entre 37 candidatos seleccionados. Los porcentajes variaron entre el 66,7% en ‘Rubidoux’ y el 12,5% en ‘Citrumelo Swingle’. La citometría demostró alta eficiencia en la detección, mientras que la colorimetría foliar permitió diferenciar diploides y tetraploides con variaciones destacadas en ‘X639’ (L* = 5.1; a* = –3.5; b* = 7.6). Estos hallazgos brindan herramientas clave para optimizar la selección de portainjertos en la citricultura moderna. Instituto de Botánica del Nordeste 2025-08-14 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unne.edu.ar/index.php/bon/article/view/8556 10.30972/bon.3428556 Bonplandia; Vol. 34 No. 2 (2025); 205-216 Bonplandia; Vol. 34 Núm. 2 (2025); 205-216 1853-8460 0524-0476 spa https://revistas.unne.edu.ar/index.php/bon/article/view/8556/8114 Derechos de autor 2025 Bonplandia http://creativecommons.org/licenses/by/4.0
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-154
container_title_str Revistas UNNE - Universidad Nacional del Noroeste (UNNE)
language Español
format Artículo revista
topic Citrus
contenido de ADN
croma
poliploides
Chroma
Citrus
dna content
polyploids
spellingShingle Citrus
contenido de ADN
croma
poliploides
Chroma
Citrus
dna content
polyploids
Garavello, Miguel F.
Recalde, José M.
Bello, Fernando
Galdeano, Florencia
Alayón Luaces, Paula
Identification and selection of tetraploid citrus rootstocks by flow cytometry and leaf colorimetry análisis
topic_facet Citrus
contenido de ADN
croma
poliploides
Chroma
Citrus
dna content
polyploids
author Garavello, Miguel F.
Recalde, José M.
Bello, Fernando
Galdeano, Florencia
Alayón Luaces, Paula
author_facet Garavello, Miguel F.
Recalde, José M.
Bello, Fernando
Galdeano, Florencia
Alayón Luaces, Paula
author_sort Garavello, Miguel F.
title Identification and selection of tetraploid citrus rootstocks by flow cytometry and leaf colorimetry análisis
title_short Identification and selection of tetraploid citrus rootstocks by flow cytometry and leaf colorimetry análisis
title_full Identification and selection of tetraploid citrus rootstocks by flow cytometry and leaf colorimetry análisis
title_fullStr Identification and selection of tetraploid citrus rootstocks by flow cytometry and leaf colorimetry análisis
title_full_unstemmed Identification and selection of tetraploid citrus rootstocks by flow cytometry and leaf colorimetry análisis
title_sort identification and selection of tetraploid citrus rootstocks by flow cytometry and leaf colorimetry análisis
description Argentina is a major citrus-producing country on the global scale, with cultivation primarily concentrated in the Argentina Northwest (NOA) and Northeast (NEA) regions. Modern citrus production relies on the use of rootstocks as a critical tool to improve tree uniformity, yield, and resilience under adverse biotic and abiotic conditions. This study focused on identifying and selecting spontaneous tetraploid individuals among five rootstock genotypes ‘Trifolio Concordia’ [Poncirus trifoliata (L.) Raf.], ‘Rubidoux’ (P. trifoliata), ‘X639’ [Citrus reshni Hort. ex Tanaka × P. trifoliata], ‘Citrange Troyer’ [C. sinensis (L.) Osbeck × P. trifoliata] y ‘Citrumelo Swingle’ (Citrus paradisi Macfad. × P. trifoliata). Through preliminary morphological screening and subsequent flow cytometry analysis, thirteen tetraploid individuals were confirmed among thirty-seven candidates. Tetraploid frequencies ranged from 66.7% in ‘Rubidoux’ to 12.5% in ‘Citrumelo Swingle’. Flow cytometry demonstrated high efficacy for ploidy determination, while leaf colorimetry effectively discriminated between diploid and tetraploid plants, with the most pronounced differences observed in ‘X639’ (L* = 5.1; a* = –3.5; b* = 7.6). These findings provide a solid basis for future research and applications in the citrus industry.
publisher Instituto de Botánica del Nordeste
publishDate 2025
url https://revistas.unne.edu.ar/index.php/bon/article/view/8556
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