Identification and selection of tetraploid citrus rootstocks by flow cytometry and leaf colorimetry análisis
Argentina is a major citrus-producing country on the global scale, with cultivation primarily concentrated in the Argentina Northwest (NOA) and Northeast (NEA) regions. Modern citrus production relies on the use of rootstocks as a critical tool to improve tree uniformity, yield, and resilience under...
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| Autores principales: | , , , , |
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| Formato: | Artículo revista |
| Lenguaje: | Español |
| Publicado: |
Instituto de Botánica del Nordeste
2025
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| Materias: | |
| Acceso en línea: | https://revistas.unne.edu.ar/index.php/bon/article/view/8556 |
| Aporte de: |
| Sumario: | Argentina is a major citrus-producing country on the global scale, with cultivation primarily concentrated in the Argentina Northwest (NOA) and Northeast (NEA) regions. Modern citrus production relies on the use of rootstocks as a critical tool to improve tree uniformity, yield, and resilience under adverse biotic and abiotic conditions. This study focused on identifying and selecting spontaneous tetraploid individuals among five rootstock genotypes ‘Trifolio Concordia’ [Poncirus trifoliata (L.) Raf.], ‘Rubidoux’ (P. trifoliata), ‘X639’ [Citrus reshni Hort. ex Tanaka × P. trifoliata], ‘Citrange Troyer’ [C. sinensis (L.) Osbeck × P. trifoliata] y ‘Citrumelo Swingle’ (Citrus paradisi Macfad. × P. trifoliata). Through preliminary morphological screening and subsequent flow cytometry analysis, thirteen tetraploid individuals were confirmed among thirty-seven candidates. Tetraploid frequencies ranged from 66.7% in ‘Rubidoux’ to 12.5% in ‘Citrumelo Swingle’. Flow cytometry demonstrated high efficacy for ploidy determination, while leaf colorimetry effectively discriminated between diploid and tetraploid plants, with the most pronounced differences observed in ‘X639’ (L* = 5.1; a* = –3.5; b* = 7.6). These findings provide a solid basis for future research and applications in the citrus industry. |
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