Search for biological activity of proteolytic enzymes isolates from native and cultivated Bromeliaceae in Corrientes on phytopathogenic microorganisms

The aim of this work was to evaluate the biological activity of protein isolates from the native Bromelia serra (BS) and cultivated bromeliad Ananas comosus (AC) on phytopathogenic microorganisms. Samples of AC were obtained from the experimental station of the Universidad Nacional del Nordeste in C...

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Autores principales: Gómez Herrera, Melanie D., Cardozo, Marina C., Prieto, María C., Avanza, María V., Alayón Luaces, Paula
Formato: Artículo revista
Lenguaje:Inglés
Publicado: Instituto de Botánica del Nordeste 2025
Materias:
Ananas comosus
Bromelia serra
natural control
control natural
Acceso en línea:https://revistas.unne.edu.ar/index.php/bon/article/view/7883
Aporte de:
Revistas UNNE - Universidad Nacional del Noroeste (UNNE) de Universidad Nacional del Nordeste
id I48-R154-article-7883
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institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-154
container_title_str Revistas UNNE - Universidad Nacional del Noroeste (UNNE)
language Inglés
format Artículo revista
topic Ananas comosus
Bromelia serra
natural control
Ananas comosus
Bromelia serra
control natural
spellingShingle Ananas comosus
Bromelia serra
natural control
Ananas comosus
Bromelia serra
control natural
Gómez Herrera, Melanie D.
Cardozo, Marina C.
Prieto, María C.
Avanza, María V.
Alayón Luaces, Paula
Search for biological activity of proteolytic enzymes isolates from native and cultivated Bromeliaceae in Corrientes on phytopathogenic microorganisms
topic_facet Ananas comosus
Bromelia serra
natural control
Ananas comosus
Bromelia serra
control natural
author Gómez Herrera, Melanie D.
Cardozo, Marina C.
Prieto, María C.
Avanza, María V.
Alayón Luaces, Paula
author_facet Gómez Herrera, Melanie D.
Cardozo, Marina C.
Prieto, María C.
Avanza, María V.
Alayón Luaces, Paula
author_sort Gómez Herrera, Melanie D.
title Search for biological activity of proteolytic enzymes isolates from native and cultivated Bromeliaceae in Corrientes on phytopathogenic microorganisms
title_short Search for biological activity of proteolytic enzymes isolates from native and cultivated Bromeliaceae in Corrientes on phytopathogenic microorganisms
title_full Search for biological activity of proteolytic enzymes isolates from native and cultivated Bromeliaceae in Corrientes on phytopathogenic microorganisms
title_fullStr Search for biological activity of proteolytic enzymes isolates from native and cultivated Bromeliaceae in Corrientes on phytopathogenic microorganisms
title_full_unstemmed Search for biological activity of proteolytic enzymes isolates from native and cultivated Bromeliaceae in Corrientes on phytopathogenic microorganisms
title_sort search for biological activity of proteolytic enzymes isolates from native and cultivated bromeliaceae in corrientes on phytopathogenic microorganisms
description The aim of this work was to evaluate the biological activity of protein isolates from the native Bromelia serra (BS) and cultivated bromeliad Ananas comosus (AC) on phytopathogenic microorganisms. Samples of AC were obtained from the experimental station of the Universidad Nacional del Nordeste in Corrientes, Argentina and of BS from natural forests also in Corrientes, Argentina. Extracts of stem and leaves of AC and leaves of BS were processed through a precipitation with acetone, the pellet was re-suspended in autoclaved buffers and called protein isolates. The antimicrobial activity was evaluated by the disc diffusion technique, using phytopathogenic bacteria. However, an inhibition zone was not observed in any of the treatments with protein isolates, unlike the positive control with Streptomycin. The minimum inhibitory concentration of bacterial growth was determined by broth microdilution technique. The results showed that the protein isolates did not inhibit bacterial growth. Furthermore, antifungal activity against Fusarium oxysporum was evaluated by MTT method, but no significant inhibition was observed either. Although the protein extracts did not show biological activity against the microorganisms evaluated, future experiments will continue the evaluation by using other microorganisms. In addition, after a purification scheme will be established, different operational conditions and tests will be used with pure enzymes instead of their extracts.
publisher Instituto de Botánica del Nordeste
publishDate 2025
url https://revistas.unne.edu.ar/index.php/bon/article/view/7883
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first_indexed 2025-05-17T05:11:33Z
last_indexed 2025-06-17T05:00:30Z
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spelling I48-R154-article-78832025-05-29T23:06:16Z Search for biological activity of proteolytic enzymes isolates from native and cultivated Bromeliaceae in Corrientes on phytopathogenic microorganisms Search for biological activity of proteolytic enzymes isolates from native and cultivated Bromeliaceae in Corrientes on phytopathogenic microorganisms Búsqueda de actividad biológica de aislados de fitoproteasas de Bromeliaceae nativas y cultivadas en Corrientes sobre microorganismos fitopatógenos Gómez Herrera, Melanie D. Cardozo, Marina C. Prieto, María C. Avanza, María V. Alayón Luaces, Paula Ananas comosus Bromelia serra natural control Ananas comosus Bromelia serra control natural The aim of this work was to evaluate the biological activity of protein isolates from the native Bromelia serra (BS) and cultivated bromeliad Ananas comosus (AC) on phytopathogenic microorganisms. Samples of AC were obtained from the experimental station of the Universidad Nacional del Nordeste in Corrientes, Argentina and of BS from natural forests also in Corrientes, Argentina. Extracts of stem and leaves of AC and leaves of BS were processed through a precipitation with acetone, the pellet was re-suspended in autoclaved buffers and called protein isolates. The antimicrobial activity was evaluated by the disc diffusion technique, using phytopathogenic bacteria. However, an inhibition zone was not observed in any of the treatments with protein isolates, unlike the positive control with Streptomycin. The minimum inhibitory concentration of bacterial growth was determined by broth microdilution technique. The results showed that the protein isolates did not inhibit bacterial growth. Furthermore, antifungal activity against Fusarium oxysporum was evaluated by MTT method, but no significant inhibition was observed either. Although the protein extracts did not show biological activity against the microorganisms evaluated, future experiments will continue the evaluation by using other microorganisms. In addition, after a purification scheme will be established, different operational conditions and tests will be used with pure enzymes instead of their extracts. El objetivo de este estudio es evaluar la actividad biológica de aislados proteicos de la planta nativa Bromelia serra (BS) y cultivada Ananas comosus (AC) sobre microorganismos fitopatógenos. Se obtuvieron muestras de bromeliáceas cultivadas (AC) del Campo Experimental de la Facultad de Ciencias Agrarias (UNNE) y de la bromeliácea nativa BS de montes naturales de la región. Se prepararon extractos de tallo y hojas de AC y hojas de BS, los cuales después de ser precipitados con acetona y obtener pellets, fueron resuspendidos en buffer estéril, los cuales se denominaron aislados proteicos. Se evaluó la actividad antimicrobiana frente a bacterias fitopatógenas mediante la técnica de difusión en disco. Sin embargo, no se observó la formación de un halo de inhibición en ninguno de los tratamientos con aislados proteicos, a diferencia del control positivo con estreptomicina. Se realizó la concentración inhibitoria mínima de crecimiento bacteriano por la técnica de microdilución en caldo. Los resultados mostraron que  los  aislados  proteicos  no  inhibieron  el  crecimiento  bacteriano. Además,  se  evaluó  la actividad antifúngica contra Fusarium oxysporum mediante la técnica del MTT, pero tampoco se observó inhibición. Aunque los extractos de proteínas no mostraron actividad biológica contra los microorganismos evaluados, futuros experimentos continuarán la evaluación utilizando otros microorganismos. Además, una vez que se establezca un esquema de purificación, se utilizarán diferentes condiciones operativas y pruebas con enzimas puras en lugar de sus extractos. Instituto de Botánica del Nordeste 2025-05-22 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unne.edu.ar/index.php/bon/article/view/7883 10.30972/bon.3417883 Bonplandia; Vol. 34 No. 1 (2025); 13-23 Bonplandia; Vol. 34 Núm. 1 (2025); 13-23 1853-8460 0524-0476 eng https://revistas.unne.edu.ar/index.php/bon/article/view/7883/7839 Derechos de autor 2024 Bonplandia http://creativecommons.org/licenses/by/4.0

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