Characterization and potential industrial application of enzymatic extracts from pacu (Piaractus mesopotamicus)
Fish production can generate considerable amounts of waste, whose inadequate final disposition could cause environmental damage. The use of these wastes to obtain high value-added products of industrial relevance is an alternative for sustainable aquaculture production. In this study, acidic extract...
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| Formato: | Artículo revista |
| Lenguaje: | Español |
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Universidad Nacional del Nordeste
2023
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| Acceso en línea: | https://revistas.unne.edu.ar/index.php/vet/article/view/6611 |
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I48-R154-article-6611 |
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| record_format |
ojs |
| institution |
Universidad Nacional del Nordeste |
| institution_str |
I-48 |
| repository_str |
R-154 |
| container_title_str |
Revistas UNNE - Universidad Nacional del Noroeste (UNNE) |
| language |
Español |
| format |
Artículo revista |
| topic |
Byproducts Piaractus mesopotamicus enzymatic extracts pepsin trypsin industrial applications Subproductos Piaractus mesopotamicus extractos enzimáticos pepsina tripsina aplicaciones industriales |
| spellingShingle |
Byproducts Piaractus mesopotamicus enzymatic extracts pepsin trypsin industrial applications Subproductos Piaractus mesopotamicus extractos enzimáticos pepsina tripsina aplicaciones industriales Acevedo Gomez, A. V. Gomez, G. N. Medina, D. M. Bogado, C. Bustillo, S. Leiva, L. C. Characterization and potential industrial application of enzymatic extracts from pacu (Piaractus mesopotamicus) |
| topic_facet |
Byproducts Piaractus mesopotamicus enzymatic extracts pepsin trypsin industrial applications Subproductos Piaractus mesopotamicus extractos enzimáticos pepsina tripsina aplicaciones industriales |
| author |
Acevedo Gomez, A. V. Gomez, G. N. Medina, D. M. Bogado, C. Bustillo, S. Leiva, L. C. |
| author_facet |
Acevedo Gomez, A. V. Gomez, G. N. Medina, D. M. Bogado, C. Bustillo, S. Leiva, L. C. |
| author_sort |
Acevedo Gomez, A. V. |
| title |
Characterization and potential industrial application of enzymatic extracts from pacu (Piaractus mesopotamicus) |
| title_short |
Characterization and potential industrial application of enzymatic extracts from pacu (Piaractus mesopotamicus) |
| title_full |
Characterization and potential industrial application of enzymatic extracts from pacu (Piaractus mesopotamicus) |
| title_fullStr |
Characterization and potential industrial application of enzymatic extracts from pacu (Piaractus mesopotamicus) |
| title_full_unstemmed |
Characterization and potential industrial application of enzymatic extracts from pacu (Piaractus mesopotamicus) |
| title_sort |
characterization and potential industrial application of enzymatic extracts from pacu (piaractus mesopotamicus) |
| description |
Fish production can generate considerable amounts of waste, whose inadequate final disposition could cause environmental damage. The use of these wastes to obtain high value-added products of industrial relevance is an alternative for sustainable aquaculture production. In this study, acidic extracts rich in pepsin-like enzymes (EP) and alkaline extracts rich in trypsin-like enzymes (ET) were obtained and characterized from the viscera of pacu (Piaractus mesopotamicus), evaluating their potential biotechnological applications. EP and ET showed maximum catalytic activity at pH 2 - 45°C and pH 7 - 45°C, respectively. They were stable in pH ranges of 1.0 to 3.5 and 7.5 to 10.5 and temperature ranges of 0 to 37°C and 0 to 45°C, respectively. The activity of EP was inhibited by pepstatin A, but not by PMSF and SBTI. EDTA and SDS caused partial inhibition, confirming the presence of pepsin-like enzymes. ET was inactivated in the presence of SBTI but not by SDS. PMSF and EDTA decreased the activity by 50%, confirming the presence of trypsin-like enzymes. Divalent ions (Ca2+ and Mg2+) did not alter the proteolytic capacity of the extracts, but it decreased with increasing NaCl concentration. Moreover, ET and EP were effective in hydrolyzing the silver-impregnated gelatin coating of discarded x-ray films and generating F(ab’)2 fragments from equine IgG degradation, respectively. In conclusion, these extracts could be applicable in processes requiring maximum activity at moderate temperatures and low NaCl concentrations, in the recovery of metallic silver and PET sheet from used x-ray films and in the production of biological products. These results open up new perspectives for the circular economy of pacú aquaculture farms. |
| publisher |
Universidad Nacional del Nordeste |
| publishDate |
2023 |
| url |
https://revistas.unne.edu.ar/index.php/vet/article/view/6611 |
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2024-08-21T22:35:49Z |
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I48-R154-article-66112023-06-12T19:14:02Z Characterization and potential industrial application of enzymatic extracts from pacu (Piaractus mesopotamicus) Caracterización y potencial aplicación industrial de extractos enzimáticos de pacú (Piaractus mesopotamicus) Acevedo Gomez, A. V. Gomez, G. N. Medina, D. M. Bogado, C. Bustillo, S. Leiva, L. C. Byproducts Piaractus mesopotamicus enzymatic extracts pepsin trypsin industrial applications Subproductos Piaractus mesopotamicus extractos enzimáticos pepsina tripsina aplicaciones industriales Fish production can generate considerable amounts of waste, whose inadequate final disposition could cause environmental damage. The use of these wastes to obtain high value-added products of industrial relevance is an alternative for sustainable aquaculture production. In this study, acidic extracts rich in pepsin-like enzymes (EP) and alkaline extracts rich in trypsin-like enzymes (ET) were obtained and characterized from the viscera of pacu (Piaractus mesopotamicus), evaluating their potential biotechnological applications. EP and ET showed maximum catalytic activity at pH 2 - 45°C and pH 7 - 45°C, respectively. They were stable in pH ranges of 1.0 to 3.5 and 7.5 to 10.5 and temperature ranges of 0 to 37°C and 0 to 45°C, respectively. The activity of EP was inhibited by pepstatin A, but not by PMSF and SBTI. EDTA and SDS caused partial inhibition, confirming the presence of pepsin-like enzymes. ET was inactivated in the presence of SBTI but not by SDS. PMSF and EDTA decreased the activity by 50%, confirming the presence of trypsin-like enzymes. Divalent ions (Ca2+ and Mg2+) did not alter the proteolytic capacity of the extracts, but it decreased with increasing NaCl concentration. Moreover, ET and EP were effective in hydrolyzing the silver-impregnated gelatin coating of discarded x-ray films and generating F(ab’)2 fragments from equine IgG degradation, respectively. In conclusion, these extracts could be applicable in processes requiring maximum activity at moderate temperatures and low NaCl concentrations, in the recovery of metallic silver and PET sheet from used x-ray films and in the production of biological products. These results open up new perspectives for the circular economy of pacú aquaculture farms. La producción pesquera puede generar cantidades considerables de residuos cuya disposición final inadecuada puede causar daños ambientales. La utilización de estos desechos para la obtención de productos con alto valor agregado y de interés industrial, es una alternativa para la producción acuícola sostenible. En este trabajo se obtuvieron y caracterizaron extractos ácidos ricos en enzimas tipo pepsina (EP) y alcalinos ricos en enzimas tipo tripsina (ET) a partir de vísceras de pacú (Piaractus mesopotamicus) evaluando sus potenciales aplicaciones biotecnológicas. EP y ET exhibieron máxima actividad catalítica a pH 2 - 45°C y pH 7 - 45°C; mostraron ser estables en los rangos de pH 1,0 a 3,5 y 7,5 a 10,5 y de temperatura 0 a 37°C y 0 a 45°C, respectivamente. La actividad de EP fue inhibida por pepstatina A pero no por PMSF y SBTI; EDTA y SDS provocaron inhibición parcial, confirmando la presencia de enzimas de tipo pepsina. ET se inactivó en presencia de SBTI pero no por SDS; PMSF y EDTA disminuyeron en un 50% su actividad, confirmando la existencia de enzimas tipo tripsina. Los iones divalentes (Ca2+ y Mg2+) no modificaron la capacidad proteolítica de los extractos, en cambio se redujo a concentraciones crecientes de NaCl. Por otra parte, ET y EP fueron eficaces para hidrolizar el recubrimiento de gelatina impregnado con plata de placas radiográficas usadas y generar fragmentos F(ab’)2 a partir de la degradación de IgG equinas, respectivamente. En conclusión, estos extractos podrían ser aplicables en procesos donde se requiera máxima actividad a temperatura moderada y baja concentración de NaCl; en la recuperación de plata metálica y celuloide de placas radiográficas reveladas como así también la obtención de productos biológicos. Estos resultados abren nuevas perspectivas para la economía circular de las explotaciones acuícolas del pacú. Universidad Nacional del Nordeste 2023-06-05 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unne.edu.ar/index.php/vet/article/view/6611 10.30972/vet.3416611 Revista Veterinaria; Vol. 34 Núm. 1 (2023); 52-61 1669-6840 1668-4834 spa https://revistas.unne.edu.ar/index.php/vet/article/view/6611/6151 Derechos de autor 2023 Revista Veterinaria |