Effect of glutamine and branched-chain amino acid supplementation on hematological and biochemical parameters of canine puppies
The objective of the study was to determine the influence of glutamine (GLN) and BCCAs (leucine, isoleucine and valine) on the hematological and biochemical parameters of the puppies. 31 mixed puppies of 30 days were recruited, from four litters, randomly in each litter a control group was establish...
Guardado en:
| Autores principales: | , , , |
|---|---|
| Formato: | Artículo revista |
| Lenguaje: | Español |
| Publicado: |
Universidad Nacional del Nordeste
2022
|
| Materias: | |
| Acceso en línea: | https://revistas.unne.edu.ar/index.php/vet/article/view/6178 |
| Aporte de: |
| id |
I48-R154-article-6178 |
|---|---|
| record_format |
ojs |
| spelling |
I48-R154-article-61782023-06-21T15:55:15Z Effect of glutamine and branched-chain amino acid supplementation on hematological and biochemical parameters of canine puppies Efecto de la glutamina y aminoácidos de cadena ramificada en los parámetros hematológicos y bioquímicos de cachorros caninos Mena, R. P. Llumiquinga, T. I. Quisirumbay, J. R. Villanueva, M. E. Cachorros Células sanguíneas Aminoácidos Suplementación Hematología Puppies Blood cells Amino acids Supplementation Hematology The objective of the study was to determine the influence of glutamine (GLN) and BCCAs (leucine, isoleucine and valine) on the hematological and biochemical parameters of the puppies. 31 mixed puppies of 30 days were recruited, from four litters, randomly in each litter a control group was established (without supplement) and 3 treatments: T1 (GLN 0.5 g/kg/day/PO), T2 (BCAAs, 0.25 /kg/ day/PO) and T3 (GLN 0.5 g/kg + BCAAs 0.25 g/kg/day/PO), were maintained for 90 days in a controlled environment under equal conditions, fed twice daily individually, samples were taken at 30 and 120 days of age. A randomized block design was applied using the litter as a block factor, descriptive statistics and normality tests were performed, as well as ANOVA to verify variations between treatments and tukey to position the treatments. At 30 days, the means and ranges of the hematological and biochemical parameters were similar. At 120 days in the hematological parameters, differences were determined (p<0.05) depending on the block, not the treatment (p>0.05), however, the average of leukocytes (10.33 10 * 9 /L) and the average of Neutrophils (7.17 10 * 9/L) of T2 were higher than the average of the control group and of the other treatments, showing a significant difference (p<0.05) only at a CI of 90%. The blood biochemistry parameters did not show differences (p>0.05). In conclusion, a positive effect on the innate immunity was evidenced, with BCAAs supplementation being favorable, with no evidence of toxicity. El objetivo del estudio fue determinar la influencia de glutamina (GLN) y BCAAs (leucina, isoleucina y valina) sobre los parámetros hematológicos y bioquímicos de los cachorros. Se reclutaron 31 cachorros mestizos de 30 días, provenientes de cuatro camadas. Aleatoriamente en cada camada se estableció un grupo control (sin suplemento) y 3 tratamientos: T1 (GLN 0,5 g/kg/día/PO), T2 (BCAAs, 0.25 g/kg/día/PO) y T3 (GLN 0,5 g/kg+BCAAs 0,25 g/kg/día/PO), que se mantuvieron 90 días en ambiente controlado en igualdad de condiciones, alimentados dos veces al día individualmente, las muestras se tomaron a los 30 y 120 días de edad. Se aplicó un diseño en bloques al azar empleando a la camada como factor de bloque, se realizaron estadísticas descriptivas y pruebas de normalidad, además ANOVA para verificar variaciones entre tratamientos y Tukey para posicionar los tratamientos. A los 30 días las medias y rangos de los parámetros hematológicos y bioquímicos fueron similares. A los 120 días en los parámetros hematológicos se determinaron diferencias (p<0.05) en función del bloque, no así del tratamiento (p>0.05), sin embargo, el promedio de leucocitos (10.33 10*9/L) y el promedio de neutrófilos (7.17 10*9/L) del T2 fueron superiores al promedio del grupo control y de los otros tratamientos mostrando diferencia significativa (p<0.05) solo a un IC del 90%. Los parámetros de la bioquímica sanguínea no mostraron diferencias (p>0.05). En conclusión, se evidenció un efecto positivo en la inmunidad innata, resultando favorable la suplementación con BCAAs, sin evidencia de toxicidad. Universidad Nacional del Nordeste 2022-12-26 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unne.edu.ar/index.php/vet/article/view/6178 10.30972/vet.3326178 Revista Veterinaria; Vol. 33 Núm. 2 (2022); 169-176 1669-6840 1668-4834 spa https://revistas.unne.edu.ar/index.php/vet/article/view/6178/5849 Derechos de autor 2022 Revista Veterinaria |
| institution |
Universidad Nacional del Nordeste |
| institution_str |
I-48 |
| repository_str |
R-154 |
| container_title_str |
Revistas UNNE - Universidad Nacional del Noroeste (UNNE) |
| language |
Español |
| format |
Artículo revista |
| topic |
Cachorros Células sanguíneas Aminoácidos Suplementación Hematología Puppies Blood cells Amino acids Supplementation Hematology |
| spellingShingle |
Cachorros Células sanguíneas Aminoácidos Suplementación Hematología Puppies Blood cells Amino acids Supplementation Hematology Mena, R. P. Llumiquinga, T. I. Quisirumbay, J. R. Villanueva, M. E. Effect of glutamine and branched-chain amino acid supplementation on hematological and biochemical parameters of canine puppies |
| topic_facet |
Cachorros Células sanguíneas Aminoácidos Suplementación Hematología Puppies Blood cells Amino acids Supplementation Hematology |
| author |
Mena, R. P. Llumiquinga, T. I. Quisirumbay, J. R. Villanueva, M. E. |
| author_facet |
Mena, R. P. Llumiquinga, T. I. Quisirumbay, J. R. Villanueva, M. E. |
| author_sort |
Mena, R. P. |
| title |
Effect of glutamine and branched-chain amino acid supplementation on hematological and biochemical parameters of canine puppies |
| title_short |
Effect of glutamine and branched-chain amino acid supplementation on hematological and biochemical parameters of canine puppies |
| title_full |
Effect of glutamine and branched-chain amino acid supplementation on hematological and biochemical parameters of canine puppies |
| title_fullStr |
Effect of glutamine and branched-chain amino acid supplementation on hematological and biochemical parameters of canine puppies |
| title_full_unstemmed |
Effect of glutamine and branched-chain amino acid supplementation on hematological and biochemical parameters of canine puppies |
| title_sort |
effect of glutamine and branched-chain amino acid supplementation on hematological and biochemical parameters of canine puppies |
| description |
The objective of the study was to determine the influence of glutamine (GLN) and BCCAs (leucine, isoleucine and valine) on the hematological and biochemical parameters of the puppies. 31 mixed puppies of 30 days were recruited, from four litters, randomly in each litter a control group was established (without supplement) and 3 treatments: T1 (GLN 0.5 g/kg/day/PO), T2 (BCAAs, 0.25 /kg/ day/PO) and T3 (GLN 0.5 g/kg + BCAAs 0.25 g/kg/day/PO), were maintained for 90 days in a controlled environment under equal conditions, fed twice daily individually, samples were taken at 30 and 120 days of age. A randomized block design was applied using the litter as a block factor, descriptive statistics and normality tests were performed, as well as ANOVA to verify variations between treatments and tukey to position the treatments. At 30 days, the means and ranges of the hematological and biochemical parameters were similar. At 120 days in the hematological parameters, differences were determined (p<0.05) depending on the block, not the treatment (p>0.05), however, the average of leukocytes (10.33 10 * 9 /L) and the average of Neutrophils (7.17 10 * 9/L) of T2 were higher than the average of the control group and of the other treatments, showing a significant difference (p<0.05) only at a CI of 90%. The blood biochemistry parameters did not show differences (p>0.05). In conclusion, a positive effect on the innate immunity was evidenced, with BCAAs supplementation being favorable, with no evidence of toxicity. |
| publisher |
Universidad Nacional del Nordeste |
| publishDate |
2022 |
| url |
https://revistas.unne.edu.ar/index.php/vet/article/view/6178 |
| work_keys_str_mv |
AT menarp effectofglutamineandbranchedchainaminoacidsupplementationonhematologicalandbiochemicalparametersofcaninepuppies AT llumiquingati effectofglutamineandbranchedchainaminoacidsupplementationonhematologicalandbiochemicalparametersofcaninepuppies AT quisirumbayjr effectofglutamineandbranchedchainaminoacidsupplementationonhematologicalandbiochemicalparametersofcaninepuppies AT villanuevame effectofglutamineandbranchedchainaminoacidsupplementationonhematologicalandbiochemicalparametersofcaninepuppies AT menarp efectodelaglutaminayaminoacidosdecadenaramificadaenlosparametroshematologicosybioquimicosdecachorroscaninos AT llumiquingati efectodelaglutaminayaminoacidosdecadenaramificadaenlosparametroshematologicosybioquimicosdecachorroscaninos AT quisirumbayjr efectodelaglutaminayaminoacidosdecadenaramificadaenlosparametroshematologicosybioquimicosdecachorroscaninos AT villanuevame efectodelaglutaminayaminoacidosdecadenaramificadaenlosparametroshematologicosybioquimicosdecachorroscaninos |
| first_indexed |
2023-03-18T23:06:57Z |
| last_indexed |
2024-08-21T22:35:43Z |
| _version_ |
1808038356778483712 |