Equine laminitis in vitro induced by whole snake poison and Fosfolipasa A2 of Bothrops alternatus from Argentina

Bothrops alternatus (yarará grande, víbora de la cruz) is a snake belonging to the Viperidae family and is widely distributed in Argentina. In the northeast, this snake group for the majority of the snakebite accidents. There are reports about envenomation in horses with clinical signs including swe...

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Autores principales: Teibler, G. P., Maruñak, L. S., Bogado, E. F., Pollitt, C.
Formato: Artículo revista
Lenguaje:Español
Publicado: Universidad Nacional del Nordeste 2022
Materias:
Snake poison
Basal membrane
Dermo-epidermal union
Veneno de serpiente
Membrana basal
Unión dermo-epidérmica
Acceso en línea:https://revistas.unne.edu.ar/index.php/vet/article/view/6174
Aporte de:
Revistas UNNE - Universidad Nacional del Noroeste (UNNE) de Universidad Nacional del Nordeste
id I48-R154-article-6174
record_format ojs
spelling I48-R154-article-61742023-06-21T13:27:43Z Equine laminitis in vitro induced by whole snake poison and Fosfolipasa A2 of Bothrops alternatus from Argentina Laminitis equina in vitro inducida por veneno entero y Fosfolipasa A2 de serpiente Bothrops alternatus de Argentina Teibler, G. P. Maruñak, L. S. Bogado, E. F. Pollitt, C. Snake poison Basal membrane Dermo-epidermal union Veneno de serpiente Membrana basal Unión dermo-epidérmica Bothrops alternatus (yarará grande, víbora de la cruz) is a snake belonging to the Viperidae family and is widely distributed in Argentina. In the northeast, this snake group for the majority of the snakebite accidents. There are reports about envenomation in horses with clinical signs including swelling, hemorrhage, dermo and myonecrosis at the bite sight in addition to systemic effects such as impaired blood coagulation, hemorrhage and damage to vital organs. An additional consequence in the horse is laminitis which reduces the work capacity of the survivor on a long-term basis. This occurs independent of the site where the venom was injected. laminitis induces pain and claudication, and it can evolve to deform the hoof. This study uses explants from freshly slaughtered horses as an in-vitro assay from the limbs of normal horses in order to demonstrate the ability of Bothrops snake venom to cause laminitis. Explants were incubated with whole venom and phospholipases A2 (PLA2). The objective was to reproduce laminitis in vitro to corroborate the clinical data reported in snake venom accidents. Equine laminitis induced by whole venom was characterized by damage to the lamellar dermo-epidermal junction, loosening of the lamellar basement membrane and cytotoxic effects. Epidermal basal cells showed changes in the shape and size of their nuclei and other abnormalities. Bothrops alternatus (yarará grande, víbora de la cruz) es una serpiente perteneciente a la familia Viperidae, y se encuentra ampliamente distribuida en Argentina. En el nordeste de nuestro país, esta serpiente ocasiona el mayor número de accidentes. Existen reportes de envenenamiento en equinos, siendo sus signos y síntomas más importantes: inflamación y hemorragia, dermo y mionecrosis en el área de inoculación del veneno. Por otro lado, la acción sistémica consiste en incoagulabilidad sanguínea, hemorragia y lesiones de órganos que pueden comprometer la salud del animal. Una secuela, debida a la intoxicación por esta serpiente, es la laminitis, que afecta la capacidad de trabajo del equino, independientemente del sitio de inoculación del veneno, debido a la acción sistémica del mismo. Esta patología produce dolor, claudicación, que luego puede progresar con deformación del casco. Se efectuó un estudio in vitro, tomando como modelo  experimental muestras (explantes) de pie equino. Estas fueron incubadas con veneno entero y con Fosfolipasa A2 (PLA2) del mismo veneno con el objeto de reproducir laminitis a los efectos de corroborar datos clínicos reportados en accidentes por yarará como así también proponer una metodología de estudio de laminitis en explantes de pie equino. La laminitis inducida por el veneno entero se caracterizó por la separación de la unión dermo–epidérmica, desprendimiento de membrana basal y citotoxicidad y consecuentemente desprendimiento de unión dermo–epidérmica, permaneciendo las células basales epidérmicas con alteraciones en forma y tamaño de los núcleos, entre otras anormalidades. En contraste, las muestras incubadas con PLA2, las células basales epidérmicas mostraron anomalías citoplasmáticas y nucleares, pero con separación leve de la unión dermoepidérmica y presente solo en algunas áreas de las LES. Universidad Nacional del Nordeste 2022-12-26 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unne.edu.ar/index.php/vet/article/view/6174 10.30972/vet.3326174 Revista Veterinaria; Vol. 33 Núm. 2 (2022); 146-151 1669-6840 1668-4834 spa https://revistas.unne.edu.ar/index.php/vet/article/view/6174/5845 Derechos de autor 2022 Revista Veterinaria
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-154
container_title_str Revistas UNNE - Universidad Nacional del Noroeste (UNNE)
language Español
format Artículo revista
topic Snake poison
Basal membrane
Dermo-epidermal union
Veneno de serpiente
Membrana basal
Unión dermo-epidérmica
spellingShingle Snake poison
Basal membrane
Dermo-epidermal union
Veneno de serpiente
Membrana basal
Unión dermo-epidérmica
Teibler, G. P.
Maruñak, L. S.
Bogado, E. F.
Pollitt, C.
Equine laminitis in vitro induced by whole snake poison and Fosfolipasa A2 of Bothrops alternatus from Argentina
topic_facet Snake poison
Basal membrane
Dermo-epidermal union
Veneno de serpiente
Membrana basal
Unión dermo-epidérmica
author Teibler, G. P.
Maruñak, L. S.
Bogado, E. F.
Pollitt, C.
author_facet Teibler, G. P.
Maruñak, L. S.
Bogado, E. F.
Pollitt, C.
author_sort Teibler, G. P.
title Equine laminitis in vitro induced by whole snake poison and Fosfolipasa A2 of Bothrops alternatus from Argentina
title_short Equine laminitis in vitro induced by whole snake poison and Fosfolipasa A2 of Bothrops alternatus from Argentina
title_full Equine laminitis in vitro induced by whole snake poison and Fosfolipasa A2 of Bothrops alternatus from Argentina
title_fullStr Equine laminitis in vitro induced by whole snake poison and Fosfolipasa A2 of Bothrops alternatus from Argentina
title_full_unstemmed Equine laminitis in vitro induced by whole snake poison and Fosfolipasa A2 of Bothrops alternatus from Argentina
title_sort equine laminitis in vitro induced by whole snake poison and fosfolipasa a2 of bothrops alternatus from argentina
description Bothrops alternatus (yarará grande, víbora de la cruz) is a snake belonging to the Viperidae family and is widely distributed in Argentina. In the northeast, this snake group for the majority of the snakebite accidents. There are reports about envenomation in horses with clinical signs including swelling, hemorrhage, dermo and myonecrosis at the bite sight in addition to systemic effects such as impaired blood coagulation, hemorrhage and damage to vital organs. An additional consequence in the horse is laminitis which reduces the work capacity of the survivor on a long-term basis. This occurs independent of the site where the venom was injected. laminitis induces pain and claudication, and it can evolve to deform the hoof. This study uses explants from freshly slaughtered horses as an in-vitro assay from the limbs of normal horses in order to demonstrate the ability of Bothrops snake venom to cause laminitis. Explants were incubated with whole venom and phospholipases A2 (PLA2). The objective was to reproduce laminitis in vitro to corroborate the clinical data reported in snake venom accidents. Equine laminitis induced by whole venom was characterized by damage to the lamellar dermo-epidermal junction, loosening of the lamellar basement membrane and cytotoxic effects. Epidermal basal cells showed changes in the shape and size of their nuclei and other abnormalities.
publisher Universidad Nacional del Nordeste
publishDate 2022
url https://revistas.unne.edu.ar/index.php/vet/article/view/6174
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first_indexed 2023-03-18T23:06:57Z
last_indexed 2024-08-21T22:35:42Z
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